An upstream region of the mouse ZP3 gene directs expression of firefly luciferase specifically to growing oocytes in transgenic mice.

Lira, Sérgio A.; Kinloch, Ross A.; Mortillo, Steven; Wassarman, Paul M.

doi:10.1073/pnas.87.18.7215

Cited by 78 publications

(58 citation statements)

References 41 publications

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“…At 10 days after birth, a piece of tail from the resulting animals was clipped for DNA analysis. Identification of transgenic founders was conducted by PCR analysis, as previously described (29). Identification of the transgenic mice was accomplished by PCR amplification of mouse tail DNA using specific primer sets.…”

Section: Generation Of Transgenic Micementioning

confidence: 99%

Ectopic Expression of the Murine Chemokines CCL21a and CCL21b Induces the Formation of Lymph Node-Like Structures in Pancreas, But Not Skin, of Transgenic Mice

Chen¹,

Vassileva²,

Kinsley³

et al. 2002

The Journal of Immunology

182

155

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The CC chemokine CCL21 is a potent chemoattractant for lymphocytes and dendritic cells in vitro. In the murine genome there are multiple copies of CCL21 encoding two CCL21 proteins that differ from each other by one amino acid at position 65 (either a serine or leucine residue). In this report, we examine the expression pattern and biological activities of both forms of CCL21. We found that although both serine and leucine forms are expressed in most tissues examined, the former was the predominant form in lymphoid organs while the latter was predominantly expressed in nonlymphoid organs. When expressed in transgenic pancreas, both forms of CCL21 were capable of inducing the formation of lymph node-like structures composed primarily of T and B cells and a few dendritic cells. Induction of lymph node-like structures by these CCL21 proteins, however, could not be reproduced in every tissue. For instance, no lymphocyte recruitment or accumulation was observed when CCL21 was overexpressed in the skin. We conclude that both forms of CCL21 protein are biologically equivalent in promoting lymphocyte recruitment to the pancreas, and that their ability to induce the formation of lymph node-like structures is dependent on the tissues in which they are expressed.

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Section: Generation Of Transgenic Micementioning

confidence: 99%

Ectopic Expression of the Murine Chemokines CCL21a and CCL21b Induces the Formation of Lymph Node-Like Structures in Pancreas, But Not Skin, of Transgenic Mice

Chen¹,

Vassileva²,

Kinsley³

et al. 2002

The Journal of Immunology

182

155

View full text Add to dashboard Cite

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“…Plasmid pZP3/6.5-LUC (26) was digested with BamHI. This yielded a 3,052-bp fragment containing a portion of the mZP3 promoter (bp -470 to +10) linked to a 2,572-bp fragment containing the * Corresponding author.…”

Section: Methodsmentioning

confidence: 99%

“…Identification of transgenic founders was carried out by polymerase chain reaction (PCR) analysis essentially as previously described (26). A small piece of mouse (6 to 15 days of age) tail was excised and digested in 100 [L of 50 mM Tris-HCI (pH 8.3)-100 mM NaCl-5 mM EDTA-1% sodium dodecyl sulfate (SDS)-300 pLg of proteinase K for 5 h at 55°C.…”

Section: Methodsmentioning

confidence: 99%

“…Oocytes were collected individually with mouth-operated, drawn-out micropipettes and washed in fresh medium (M2), and their diameter was measured by using a digital filar eyepiece on a Labovert FS microscope (Leitz) connected to a M/XM processor (Lasico). Measured oocytes were placed individually in 50 gil of homogenization buffer (described above), stored at -80°C, and assayed for luciferase activity essentially as previously described (26).…”

Section: Methodsmentioning

confidence: 99%

“…To date, zona pellucida glycoproteins are the only well-documented examples of oocyte-specific gene expression in mammals. In this context, we found that 6.5 kb of mZP3 gene 5'-flanking region contains cis-acting elements that are responsible for regulation of oocyte-specific mZP3 gene expression (26).…”

mentioning

confidence: 94%

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A mouse oocyte-specific protein that binds to a region of mZP3 promoter responsible for oocyte-specific mZP3 gene expression.

Schickler¹,

Lira²,

Kinloch³

et al. 1992

Mol. Cell. Biol.

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The gene encoding mZP3, the mouse sperm receptor, is expressed exclusively in growing oocytes during oogenesis. To investigate the molecular basis of oocyte-specific mZP3 gene expression, we generated several lines of mice harboring a transgene that contains 470 bp of mZP3 gene 5'-flanking sequence (nucleotides -470 to +10) fused to the firefly luciferase gene coding region. Three of four expressing transgenic lines exhibited luciferase activity only in growing oocytes, suggesting that the 470-bp fragment is sufficient to direct Iocyte-specific expression of the luciferase gene. Results of DNase I footprinting and gel mobility shift assays suggested the presence of an ovary-specific protein that binds to a small region (nucleotides-99 to -86) within the 470-bp fragment of the mZP3 promoter, with 5'-G(G/A)T(G/A)A-3' representing the minimal sequence required for binding. Southwestern (DNA-protein) gel blots revealed the presence of an oocyte-specific, approximately 60,000-Mr protein, called OSP-1, that binds to the minimal sequence. Changes in levels of OSP-1 during oogenesis and early cleavage are consistent with the pattern of mZP3 gene expression during these developmental stages in mice. Therefore, OSP-1 may be a mammalian oocyte-specific transcription factor involved in regulating oocyte-specific mZP3 gene expression.

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Structure and function of the mammalian egg zona pellucida

et al. 1999

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The zona pellucida is a thick extracellular coat that surrounds all mammalian eggs and preimplantation embryos. The zona pellucida supports communication between oocytes and follicle cells during oogenesis; protects oocytes, eggs, and embryos during development, and regulates interactions between ovulated eggs and free‐swimming sperm during and following fertilization. Mutant females that produce eggs that lack a zona pellucida are infertile. The functions of the zona pellucida during fertilization now can be ascribed to certain of its glycoproteins. Here we describe some aspects of zona pellucida structure and function as they relate to mammalian fertilization. J. Exp. Zool. (Mol. Dev. Evol.) 285:251–258, 1999. © 1999 Wiley‐Liss, Inc.

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An upstream region of the mouse ZP3 gene directs expression of firefly luciferase specifically to growing oocytes in transgenic mice.

Abstract: The gene encoding the mouse egg primary receptor for sperm, a zona pellucida glycoprotein called

Cited by 78 publications

References 41 publications

Ectopic Expression of the Murine Chemokines CCL21a and CCL21b Induces the Formation of Lymph Node-Like Structures in Pancreas, But Not Skin, of Transgenic Mice

Ectopic Expression of the Murine Chemokines CCL21a and CCL21b Induces the Formation of Lymph Node-Like Structures in Pancreas, But Not Skin, of Transgenic Mice

A mouse oocyte-specific protein that binds to a region of mZP3 promoter responsible for oocyte-specific mZP3 gene expression.

Structure and function of the mammalian egg zona pellucida

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