1995
DOI: 10.1099/13500872-141-12-3067
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An X-prolyl dipeptidyl aminopeptidase (pepX) gene from Lactobacillus helveticus

Abstract: The X-prolyl dipeptidyl aminopeptidase gene (pepx) of an industrially used Lactobacillus helveticus strain has been detected by nucleic acid hybridization, cloned, characterized and sequenced. One ORF of 2379 bp with coding capacity for a 906 kDa protein (PepX) was found. The ORF was preceded by a typical prokaryotic promoter region. An inverted repeat structure with AG of -84.1 kJ mol-1 was found downstream of the coding region. The deduced amino acid sequence of the 906 kDa protein showed 49*3,494 and 777% h… Show more

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Cited by 64 publications
(49 citation statements)
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“…The substrate showing the highest affinity for the recombinant DPP IV was Gly-Pro-pNa, with a K m of 0.26 mM and a V max of 7.8 mol mg Ϫ1 min Ϫ1 . This value is comparable to those reported for P. albensis (27) and L. helveticus (26). The recombinant DPP IV also had significant affinity for Lys-AlapNa (K m of 0.87 mM) and Ala-Pro-pNa (K m of 12 mM) with V max values of 5.9 and 30 mol mg Ϫ1 min Ϫ1 , respectively.…”
Section: S Suissupporting
confidence: 70%
“…The substrate showing the highest affinity for the recombinant DPP IV was Gly-Pro-pNa, with a K m of 0.26 mM and a V max of 7.8 mol mg Ϫ1 min Ϫ1 . This value is comparable to those reported for P. albensis (27) and L. helveticus (26). The recombinant DPP IV also had significant affinity for Lys-AlapNa (K m of 0.87 mM) and Ala-Pro-pNa (K m of 12 mM) with V max values of 5.9 and 30 mol mg Ϫ1 min Ϫ1 , respectively.…”
Section: S Suissupporting
confidence: 70%
“…The degree of purification of the enzyme preparation used was of 716.6-fold. with a subunit molecular mass of 80 to 95 kDa (3,11,15,16,32,33). However, the enzymes of Lactobacillus casei and of some strains of Lactobacillus helveticus and Lactobacillus delbrueckii have been found to be monomers or trimers (2,9,17,19,20).…”
Section: Discussionmentioning
confidence: 99%
“…helveticus CNRZ 32 [38] and Lb. helveticus 53/7 [33] and 35% homology with PepX from Lb. delbrueckii ssp.…”
Section: Compoundsmentioning
confidence: 99%
“…L -1 . The inhibitory effect of Hg 2+ , Cd 2+ , Cu 2+ and Zn 2+ on PepXs characterized from other LAB has been reported [1,8,10,22,33].…”
Section: Compoundsmentioning
confidence: 99%