An α-D-galactan and a β-D-glucan from the mushroom Amanita muscaria: Structural characterization and antitumor activity against melanoma

Zavadinack, Matheus; Bellan, Daniel de Lima; Bertage, Jessica Loren da Rocha; Milhorini, Shayane da Silva; Trindade, Edvaldo da Silva; Simas, Fernanda Fogagnoli; Sassaki, Guilherme Lanzi; Cordeiro, Lucimara M.C.; Iacomini, Marcello

doi:10.1016/j.carbpol.2021.118647

Cited by 28 publications

(10 citation statements)

References 48 publications

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“…NMR spectroscopy can provide detailed structural information of complex polysaccharides according the chemical shift, including the configurations, linkage patterns and sequences of the sugar units 32 . Generally, the anomeric‐carbon proton chemical shifts of α‐configurations in the 1 H spectrum were δ 4.9–5.6 ppm, and that in the 13 C NMR spectrum was δ 95–101 ppm 33 .…”

Section: Resultsmentioning

confidence: 99%

Isolation, structure characterization and in vitro immune‐enhancing activity of a glucan from the peels of stem lettuce (Lactuca sativa)

Zou,

Shen,

et al. 2023

J Sci Food Agric

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BACKGROUNDStem lettuce is a medicinal and edible plant. The peels, accounting for 300–400 g kg−1 raw stem lettuce and containing polysaccharides 200 g kg−1, are discarded as industrial waste, causing environment pollution and resource waste.RESULTSA polysaccharide named PPSL10‐2 was obtained from the peels of stem lettuce after hot water extraction, and gradation with cascade ultrafiltration and purification using DEAE‐Sepharose cellulose. The purity and molecular weight of PPSL10‐2 is 96.10% and 2.2 × 104 Da respectively, as detected by high‐performance gel permeation chromatography. PPSL10‐2 was found to be an α‐(1→4)‐d‐glucan that branched at O‐6 with a terminal 1‐linked α‐d‐Glcp as side chain, and devoid of helix conformation, which was characterized by monosaccharide composition analysis, Fourier‐transform infrared spectroscopy, Congo red test, scanning electron microscopy, methylation analysis and NMR spectroscopy. Furthermore, PPSL10‐2 exhibited potent immune‐enhancing effect by improving proliferation and phagocytosis, promoting the secretion of nitric oxide and cytokines, as well as the expression of related genes in RAW264.7 macrophages.CONCLUSIONThe findings of the present study suggest that peels as an agricultural by‐product of stem lettuce are good sources of polysaccharides, which could be developed as immunopotentiator for improving human health. © 2023 Society of Chemical Industry.

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Section: Resultsmentioning

confidence: 99%

Isolation, structure characterization and in vitro immune‐enhancing activity of a glucan from the peels of stem lettuce (Lactuca sativa)

Zou,

Shen,

et al. 2023

J Sci Food Agric

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“…The activity of the polysaccharide was affected by the type of the backbone and the degree of branching [ 20 ]. Polysaccharides from the Amanita muscaria have a (1→6)-linked Galp main chain, which selectively inhibits the proliferation of B16-F10 melanoma cells [ 21 ]. The glycyrrhiza polysaccharides with 1,4-linked-Galp main chains promoted dendritic cells maturation, antigen presentation and phagocytosis [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory Activity and Its Mechanisms of Two Polysaccharides from Poria cocos

Zhang,

He,

Zheng

et al. 2023

Molecules

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Polyporaceae is an important fungal family that has been a source of natural products with a range of pharmaceutical activities in China. In our previous study, two polysaccharides, PCWPW and PCWPS, with significant antioxidant and antidepressant activity were obtained from Poria cocos. In this study, we evaluated their potential molecular mechanisms in the immunomodulation of macrophages. PCWPW and PCWPS were characterized by GC–MS analysis to contain 1,3-linked Glcp. ELISA assays results demonstrated that the secretion of TNF-α was significantly enhanced by PCWPW/PCWPS. RNA-seq data demonstrated that PCWPS treatment modulated the expression of immune-related genes in macrophages, which was further confirmed by RT-qPCR assays. The activation of TNF-α secretion was found to be mannose receptor (MR) dependent and suppressed by MR inhibitor pretreatment. Moreover, the amount of TNF-α cytokine secretion in PCWPW/PCWPS-induced RAW264.7 cells was decreased when pretreated with NF-κB or MAPK signaling pathway inhibitors. Collectively, our results suggested that PCWPW and PCWPS possessed immunomodulatory activity that regulates TNF-α expression through the NF-κB/MAPK signaling pathway by binding to mannose receptors. Therefore, PCWPW and PCWPS isolated from Poria cocos have potential as drug candidates for immune-related disease treatment.

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“…2B). According to the literature data as well as the results of methylation and monosaccharide composition, four main anomeric protons could be labelled as A-D, which were identified as β-D-Manp-(1→ (4.72/ 100.73 ppm), 37 →6)-α-D-Galp-(1→ (4.92/97.24 ppm), 42 →2,6)α-D-Galp-(1→ (5.06/97.76 ppm) 42 and →6)-β-D-Glcp-(1→ (4.43/ 102.06 ppm), 43 respectively. Two-dimensional NMR atlas were further analyzed to elucidate the PAPS2 structural features.…”

Section: Papermentioning

confidence: 99%

The amelioration of a purified Pleurotus abieticola polysaccharide on atherosclerosis in ApoE^−/− mice

Xing,

Kong,

Wang

et al. 2024

Food Funct.

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An α-D-galactan and a β-D-glucan from the mushroom Amanita muscaria: Structural characterization and antitumor activity against melanoma

Cited by 28 publications

References 48 publications

Isolation, structure characterization and in vitro immune‐enhancing activity of a glucan from the peels of stem lettuce (Lactuca sativa)

Isolation, structure characterization and in vitro immune‐enhancing activity of a glucan from the peels of stem lettuce (Lactuca sativa)

Immunomodulatory Activity and Its Mechanisms of Two Polysaccharides from Poria cocos

The amelioration of a purified Pleurotus abieticola polysaccharide on atherosclerosis in ApoE^−/− mice

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An α-D-galactan and a β-D-glucan from the mushroom Amanita muscaria: Structural characterization and antitumor activity against melanoma

Cited by 28 publications

References 48 publications

Isolation, structure characterization and in vitro immune‐enhancing activity of a glucan from the peels of stem lettuce (Lactuca sativa)

Isolation, structure characterization and in vitro immune‐enhancing activity of a glucan from the peels of stem lettuce (Lactuca sativa)

Immunomodulatory Activity and Its Mechanisms of Two Polysaccharides from Poria cocos

The amelioration of a purified Pleurotus abieticola polysaccharide on atherosclerosis in ApoE−/− mice

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The amelioration of a purified Pleurotus abieticola polysaccharide on atherosclerosis in ApoE^−/− mice