An β-1,4-Xylanase with Exo-Enzyme Activity Produced by Paenibacillus xylanilyticus KJ-03 and Its Cloning and Characterization

Abstract

Paenibacillus xylanilyticus KJ-03 was isolated from soil samples obtained from a field with Amorphophallus konjac plants. A gene encoding xylanase was isolated from KJ-03 and cloned using a fosmid library. The xynA gene encodes xylanase; it consists of 1,035 bp and encodes 345 amino acids. The amino acid sequence deduced from the P. xylanilyticus KJ-03 xylanase showed 81% and 69% identities with those deduced from the P. polymyxa E681 and Paenibacillus sp. HPL-001 xylanases, respectively. The xynA gene comprises a single domain, consisting of a catalytic domain of the glycosyl hydrolase (GH) 10 family. The xynA gene was expressed in Escherichia coli BL21 (trxB), and the recombinant xylanase was purified by Niaffinity chromatography. The purified xylanase showed optimum activity with birchwood xylan as a substrate at 40°C and pH 7.4. Treatment with Mg(2+) and Li(+) showed a slight decrease in XynA activity; however, treatment with 5 mM Cu(2+) completely inhibited its activity. The results of the thin layer chromatography analysis indicated that the major hydrolysis product was xylobiose and small amounts of xylose and xylotriose. XynA showed increased activity with oat spelt xylan and birchwood xylan, but showed only slight activity with locust bean gum.

Characterization of a new bifunctional endo-1,4-β-xylanase/esterase found in the rumen metagenome

“…Hydrolysis activity at room temperature is an important characteristic for the solubilization of hemicelluloses, which would not need thermal control in the range of 30 to 45 °C. This result is similar to that for xylanases obtained from other microorganisms, such as Paenibacillus xylanilyticus KJ-03 (40 °C) 42 , Sorangium cellulosum So9733-1 (30 °C) 43 , and Bacillus sp. SN5 (40 °C) 29 .…”

Bacterial xylanases: biology to biotechnology

“…SN5 BeX 40 7.0 0.6 114 Ni-affinity chromatography 104.7 – – Bai et al ( 2012 ) 3. Paenibacillus xylanilyticus KJ - 03 BiX 40 7.4 – – Ni-affinity chromatography 33 7.5 58 Park et al ( 2013 ) 4. Bacillus sp.…”

Antimicrobial Resistance, Biocide Tolerance, and Bacterial Diversity of a Dressing Made from Coriander and Parsley after Application of Treatments Using High Hydrostatic Pressure Alone or in Combination with Moderate Heat

“…Notably, identification of bacterial isolates from the pressurized samples under non-selective conditions revealed mostly Bacillus endophyticus and Paenibacillus xylanilyticus , two endospore formers associated with vegetables and soil [ 45 , 46 ]. The presence of these bacteria in the residual population after HP treatments could be explained by the higher resistance of bacterial endospores under HP conditions [ 47 ].…”