Anabolic Effects of Bee Pollen Cistus ladaniferus Extract in Osteoblastic MC3T3-E1 Cells In Vitro

Abstract

The effect of bee pollen extract on osteoblastic MC3T3-E1 cells was investigated. The watersolubilized extracts, which were obtained from the bee pollen of Cistus ladaniferus, was purified using the membrane fractionation method with molecular weight (MW) less than 1000. Osteoblastic cells were cultured for 72 hr in a medium containing either vehicle or cistus extract of less than MW 1000 (10, 25, or 50 µg/ml of medium) in the presence of 10% fetal bovine serum (FBS). The proliferation of osteoblastic cells was significantly enhanced in the presence of cistus extract (25 or 50 µg/ml). The stimulatory effect of cistus extract on cell proliferation was also observed when the cells with subconfluency were cultured for 24 or 72 hr without FBS. Cells with subconfluency were cultured for 24 or 72 hr in a medium containing either vehicle or cistus extract without FBS. DNA content or alkaline phosphatase activity in osteoblastic cells was significantly increased after culture with cistus extract (50 µg/ml) for 24 hr. The effect of 25 µg/ml of cistus extract on these components was seen after culture for 72 hr. This study demonstrates that the cistus extract fraction of less than MW 1000 has anabolic effects in osteoblastic MC3T3-E1 cells which involve in bone formation.

Polyphenols from Bee Pollen: Structure, Absorption, Metabolism and Biological Activity

“…Tests in vitro demonstrated the anabolic effects of bee pollen extracts on the osteoblastic cells of MC3T3-E1 line, confirming that bee pollen extracts stimulate osteoblastic bone formation [110].…”

Dose-Dependent Impact of Bee Pollen Supplementation on Macroscopic and Microscopic Structure of Femoral Bone in Rats

“…Our findings differ from those reported by Yamaguchi et al [ 6 ], who mentioned a higher Ca concentration and increased ALP activity in the femoral bone of rats administered with bee pollen extract obtained from Cistus ladaniferus (5 and 10 mg/100 g bw). Yamaguchi et al [ 22 ] also detected a stimulatory effect of bee pollen Cistus ladaniferus extract on bone formation and its inhibitory impact on bone resorption in vitro using osteoblast cells. However, Tomaszewska et al [ 1 ] also stated a reduced mineralization in the tibia of Japanese quails supplemented with 1.0% of multifloral bee pollen.…”

Consumption of bee pollen affects rat ovarian functions

“…Results of this study extend our previous observation of the effect of bee pollen treatment on secretion activity, as well as on markers of proliferation and apoptosis in cultured porcine ovarian granulosa cells (Kolesarova et al., 2011). The effect of bee pollen on osteoblastic cells (Yamaguchi et al., 2007) and human prostate cancer cells (Samarghandian et al., 2010) was previously studied. Present results, together with our previous observations (Kolesarova et al., 2011), demonstrate a possible effect of bee pollen on ovarian functions.…”

“…To our knowledge, before start of our studies, no studies of the effect of bee pollen on ovarian cell apoptosis and proliferation have been performed. Previously, the antiapoptotic and pro‐proliferating effect of propolis on periodontal ligament cells (Gjertsen et al., 2011), as well as the bee pollen–induced proliferation in cultured mouse osteoblasts (Yamaguchi et al., 2007), has been reported. On the other hand, addition of caffeic acid phenethyl ester, an active component isolated from propolis, inhibited growth and induced G 1 phase arrest of human colon cancer cells (Xiang et al., 2006).…”