Anabolic effects of clenbuterol after long-term treatment and withdrawal in the rat

Cartañà, J.; Segués, Teresa; Yebras, Martí; Rothwell, Nancy J.; Stock, Michael J.

doi:10.1016/0026-0495(94)90049-3

Cited by 23 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The decreases of the testicular weight and volume recorded in the clenbuterol groups with respect to the control are in accordance with the morphological observations of Groot et al [8] on testes of calves treated with clenbuterol. A decrease in the testicular weight has also been reported in rats treated with clenbuterol [3] but this would be in relation with the clenbuterol-related reduction of the epididymal fat pad observed in these animals, but the testes of the pig were not characterized for epididymal or testicular adipose deposits. On the contrary adrenergic agents have proved their ability to contract the testicular capsule [7] by providing a pumping action, which could be overstimulated in the clenbuterol-treated animals.…”

Section: Discussionmentioning

confidence: 87%

Quantitative modification of the testicular structure in pigs fed with anabolic doses of clenbuterol

et al. 2002

View full text Add to dashboard Cite

-Morphological and structural data of the testes of thirty male pigs were recorded in order to evaluate the effects of an anabolic clenbuterol treatment. Pigs aged 6 months were randomly allocated to one of three experimental groups. In two treated groups, the animals were fed with anabolic doses of clenbuterol (1 ppm); in the CLB group (n = 10) clenbuterol was given until they were slaughtered (treatment period = 3 months) whereas in the CLBW group (n = 10) the clenbuterol was withdrawn two weeks before slaughter (treatment period = 2.5 months); clenbuterol was not given to the pigs of the control group (n = 10). Stereological estimations of the tissular volume fraction and tubular volume density were applied to quantify the structural constituents of the testes. The results showed an increased volume fraction of the testicular interstitium especially in the Leydig cell population, as a result of the clenbuterol treatment. The increase in the nuclear volume fraction of the Leydig cells was the more persistent change in the variations recorded in both treated groups with respect to the control. A regression of the seminal epithelium was also recorded in the treated animals. The rest of the structural parameters were closer to the normal figures in the CLBW group, suggesting a recovery of the testicular structure when clenbuterol was withdrawn.clenbuterol / pig / testes / testicular structure / Leydig cell Résumé -Modification quantitative de la structure testiculaire chez des porcs nourris avec des doses anabolisantes de clenbutérol. Les données morphologiques et structurales des testicules de trente porcs ont été recueillies afin d'évaluer les effets d'un traitement anabolisant à base de clenbuté-rol. Les porcs ont été distribués de façon aléatoire dans chacun des trois groupes expérimentaux : dans deux des groupes traités les animaux ont été nourris avec des doses anabolisantes de clenbutérol (1 ppm); dans le groupe CLB (n = 10) le clenbutérol a été administré aux animaux jusqu'à l'abattage (période de traitement = 3 mois) alors que dans le groupe CLBW (n = 10) le clenbutérol a été retiré de 47 Vet. Res. 33 (2002) (34) 957218847; e-mail: jg.monterde@uco.es l'alimentation deux semaines avant l'abattage (période de traitement = 2,5 mois); le clenbutérol n'a pas été administré aux porcs du groupe témoin (n = 10). Les estimations stéréologiques de la fraction du volume tissulaire et de la densité du volume tubulaire ont été utilisées pour quantifier les constituants structuraux des testicules. Un accroissement de la fraction du volume de l'interstitium testiculaire et surtout de la population des cellules de Leydig (fraction de volume occupée par les noyaux) a été la modification la plus persistante des variations recueillies dans les deux groupes traités par rapport au groupe témoin. Une régression de l'épithélium séminal a également été notée chez les animaux traités. Le reste des paramètres structuraux étaient plus proches des schémas normaux dans le groupe CLBW suggérant un retour à la normale des ...

show abstract

Section: Discussionmentioning

confidence: 87%

Quantitative modification of the testicular structure in pigs fed with anabolic doses of clenbuterol

et al. 2002

View full text Add to dashboard Cite

show abstract

“…Clenbuterol produced most of the phenotypic changes previously reported in rats ( Figure 4; Reeds et al, 1988;Carter et al, 1991;Cartana et al, 1994). With clenbuterol treatment, muscle weight increased 25%, regardless of PCDD/F exposure (P ≤ 0.0001; Figure 4 Rats were either treated with corn oil (control), clenbuterol alone (clenbuterol), a polychlorinated dioxin and furan [PCDD/F] mixture (dioxin), or a PCDD/F mixture, followed by clenbuterol (dioxin + clenbuterol).…”

Section: Physiological Responses To Clenbuterol Treatmentmentioning

confidence: 85%

Effects of clenbuterol on body stores of polychlorinated dibenzofurans (PCDF) and dibenzo-p-dioxins (PCDD) in rats

Shappell

Billey

Feil

2002

Journal of Animal Science

View full text Add to dashboard Cite

Polychlorinated dibenzo-p-dioxins (PCDD) and polychlorinated dibenzofurans (PCDF), persistent pollutants that accumulate in the food chain, pose a risk to humans through consumption of tainted livestock. Clenbuterol, a leanness-enhancing agent, was tested for usefulness in PCDD/F body store reduction through body fat reduction (the predominant site of accumulation). To mimic the situation of contaminated animals, rats were given feed with or without a mixture of PCDD/F (0.6 to 2.7 ng/congener per day) for 10 d, followed by 16 d of feed with or without dietary clenbuterol (2 mg/kg feed). Clenbuterol reduced body fat by 28% (P < 0.05), increased muscle mass by 25% (P < 0.02), and decreased liver mass by 7% (P < 0.02). Although the concentrations of most PCDD/F per gram of fat were slightly increased after clenbuterol treatment, the total amount of PCDD/F that remained in fat was reduced by approximately 30%. Muscle PCDD/F concentrations and total burden were decreased by clenbuterol. In contrast, clenbuterol tended to increase concentration, but not total burden of PCDD/F in livers. One congener known to be rapidly metabolized and excreted, 2,3,7,8-TCDF, was the exception to this increase, decreasing 40% with clenbuterol treatment. This was also the congener that showed the greatest reduction in both fat and muscle. Examination of the ratio of PCDD/F in liver and fat revealed that clenbuterol increased the liver's share of the body burden of PCDD/F, from 38 to 75%. In a remediation/disposal context, these findings would be beneficial if clenbuterol lowered the meat and carcass burden of PCDD/F to safe levels, requiring only livers to be disposed of as hazardous waste.

show abstract

“…The opinion is more divided concerning the use of β 2 ‐agonists (Berges et al, 2000). Some studies have shown that systemic use in an animal model increases weight and muscle mass (MacLennan & Edwards, 1989; Cartana & Stock, 1994, 1995; Cartana et al, 1994; Ryall et al, 2002, 2006), and increases the regeneration of skeletal muscle (Beitzel et al, 2004), whereas others have not been able to demonstrate this effect (Kindermann & Meyer, 2006). On the other hand, no effect has been reported in humans on oxygen uptake, lung function and performance with the use of therapeutic doses of inhaled bronchodilator either in asthmatics or non‐asthmatics (Meeuwisse et al, 1992; Robertson et al, 1994; Heir & Stemshaug, 1995; Morton et al, 1996; Norris et al, 1996; Goubault et al, 2001).…”

Section: Anti‐asthmatic Treatment As Dopingmentioning

confidence: 99%

Pharmaceutical treatment of asthma symptoms in elite athletes – doping or therapy?

Backer

Lund

Pedersen

2007

Scandinavian Med Sci Sports

View full text Add to dashboard Cite

Asthma, exercise‐induced bronchoconstriction, and airway hyper‐responsiveness are often found in elite athletes, perhaps as a consequence of their sport or maybe because asthma is a common disorder in young adults. Inhaled β2‐agonists (IBA) are frequently used in elite athletes, but due to regulations introduced by the International Olympic Committee, the use of anti‐asthmatic therapy might change. Drugs that make ergogenic effect persist are prohibited in all athletes, whether or not they take part in competitions and systemic steroids and β2‐agonists are among such drugs. On the other hand, opinion is more divided about the use of inhaled corticosteroids (ICS) and IBA. In humans, no effect has been found on the oxygen uptake, performance or distance run with therapeutic doses of IBA, either in asthmatics or non‐asthmatics, whereas others report an ergogenic effect and better lung function of high doses of a β2‐agonist in non‐asthmatics. Anti‐asthmatic treatment is necessary for asthmatics, but should not be used by non‐asthmatic elite athletes due to both possible systemic effects and furthermore, side effects of both ICS and IBA.

show abstract

Anabolic effects of clenbuterol after long-term treatment and withdrawal in the rat

Cited by 23 publications

References 26 publications

Quantitative modification of the testicular structure in pigs fed with anabolic doses of clenbuterol

Quantitative modification of the testicular structure in pigs fed with anabolic doses of clenbuterol

Effects of clenbuterol on body stores of polychlorinated dibenzofurans (PCDF) and dibenzo-p-dioxins (PCDD) in rats

Pharmaceutical treatment of asthma symptoms in elite athletes – doping or therapy?

Contact Info

Product

Resources

About