Anabolic implants alter abundance of mRNA involved in muscle growth, metabolism, and inflammation in the longissimus of Angus steers in the feedlot

Reichhardt, Caleb C; Cuthbert, Jocelyn; Motsinger, Laura A; Brady, Tevan J; Briggs, Reganne K; Thomas, Aaron J.; Thornton, Kara J

doi:10.1016/j.domaniend.2022.106773

Cited by 2 publications

(3 citation statements)

References 73 publications

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“…Similar to our prior observation that implant administration decreases plasma Zn concentrations ( Messersmith, 2018 ; Reichhardt et al, 2022 ), implant tended to decrease plasma Zn concentrations. Plasma Zn of implanted steers decreased 9.0% from days 0 to 20.…”

Section: Discussionsupporting

confidence: 89%

“…Supplementation up to 150 mg Zn/kg DM from ZnSO 4 results in improved growth performance in the first 18 d after steers are administered a high-potency implant ( Messersmith and Hansen, 2021a ). We have also noted consistent decreases in plasma Zn concentration following implantation with a high-potency combination steroidal implant ( Messersmith, 2018 ; Messersmith et al, 2022 ; Reichhardt et al, 2022 ).…”

Section: Introductionsupporting

confidence: 66%

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Influence of steroidal implants and zinc sulfate supplementation on growth performance, trace mineral status, circulating metabolites, and transcriptional changes in skeletal muscle of feedlot steers

Smerchek,

Rients,

McLaughlin

et al. 2024

Journal of Animal Science

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Angus-cross steers (n = 144; 362 kg ± 20.4) were used to determine the effect of Zn and steroidal implants on performance, trace mineral status, circulating metabolites, and transcriptional changes occurring in skeletal muscle. Steers (n = 6/pen) were stratified by BW in a 3 × 2 factorial. GrowSafe bunks recorded individual feed intake (steer as experimental unit; n = 24/treatment). Dietary treatments (ZINC; 8 pens/treatment) included supplemental Zn as ZnSO4 at: 1) 0 (analyzed 54 mg Zn/kg DM; Zn0); 2) 30 mg/kg DM (Zn30); 3) 100 mg Zn/kg DM (Zn100). After 60 d of Zn treatment, steers received a steroidal implant treatment (IMP) on d 0: 1) no implant; NO; or 2) high potency combination implant (TE-200, Elanco, Greenfield, IN; 200 mg TBA, 20 mg E2; TE200). Body weights were taken at d -60, 0, and in 28 d increments thereafter. Liver biopsies for TM analysis and blood for TM, serum glucose, insulin, non-esterified fatty acids (NEFA), urea-N (SUN), and IGF-1 analysis were collected on d 0, 20, 40, and 84. Glucose, NEFA, and insulin were used to calculate the revised quantitative insulin sensitivity check index (RQUICKI). Linear and quadratic effects of ZINC were evaluated in SAS 9.4. Means for IMP were separated using the LSMEANS statement with the PDIFF option. Day -60 BW was a covariate for performance and carcass data. Growth performance, plasma, liver, and metabolite data were analyzed as repeated measures. TE200 tended to decrease plasma Zn 8.4% from d 0 to 20 while NO decreased 3.6% (IMP × day; P = 0.08). A tendency for a ZINC × day effect on G:F was noted (P = 0.06) driven by Zn30 and Zn100 decreasing significantly from period 0-28 to period 28-56 while Zn0 was similar in both periods. An IMP × day effect was noted for RQUICKI where (P = 0.02) TE200 was greater on d 40 compared to NO cattle, but by d 84 RQUICKI was not different between TE200 and NO. On d 20, increasing Zn supplementation linearly increased mRNA abundance (P ≤ 0.09) of protein kinase B (AKT1), mammalian target of rapamycin (mTOR), matrix metalloproteinase 2 (MMP2) and myogenic factor 5 (MYF5). In this study, Zn and implants differentially affected genes related to energy metabolism, satellite cell function, and TM homeostasis on d 20 and 84 post-implant. These results suggest steroidal implants increase demand for Zn immediately following implant administration to support growth and may influence insulin sensitivity in finishing cattle.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionsupporting

confidence: 66%

Influence of steroidal implants and zinc sulfate supplementation on growth performance, trace mineral status, circulating metabolites, and transcriptional changes in skeletal muscle of feedlot steers

Smerchek,

Rients,

McLaughlin

et al. 2024

Journal of Animal Science

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“…RNA was isolated from longissimus thoracis and cDNA synthesized as described in Rients et al (2023). Quantitative real-time PCR was performed using Fluidigm Biomark HD system as described in Reichhardt et al (2023). Primers utilized for qPCR are found in Supplementary Table 1.…”

Section: Methodsmentioning

confidence: 99%

Zinc supplementation and ractopamine hydrochloride impact gene expression of zinc transporters in finishing beef steers

et al. 2023

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Zinc is a trace mineral of interest for optimizing growth in feedlot cattle due to its roles in many physiological functions, including growth. Twenty-four Angus-cross steers (467 ± 13 kg) were used to assess the effects of supplemental Zn and ractopamine hydrochloride (RAC) on trace mineral concentrations and muscle gene expression. Four GrowSafe-equipped pens were randomly assigned to treatments (1 pen of six steers/treatment): 0 (CON), 60 (LOW), 120 (MED) or 180 (HI) mg supplemental Zn/kg DM (Availa-Zn, Zinpro). Dietary Zn treatments were initiated on d 0 and RAC supplementation (300 mg·steer·-1·d-1; Actogain45, Zoetis) began on d 53. Blood, liver and muscle (longissimus thoracis) samples were collected from all steers on d -4, 48, and 67. The LOW treatment was removed from gene expression analyses due to < 3 steers being represented for 14 of 22 genes. Data were analyzed using ProcMixed of SAS with the fixed effect of treatment and steer as the experimental unit; orthogonal linear and quadratic contrast statements were used to compare treatments. On d 48 and 67, there were linear and quadratic trends for plasma Zn to be greater in Zn-supplemented steers than CON (P ≤ 0.10). On d 48, there was a tendency for a quadratic decrease on the expression of SLC30A4 (P ≤ 0.07) but no other differences due to treatment. On d 67, several genes involved in Zn transport and storage (MTA1, SLC39A7, SLC39A8, SLC39A9, SLC39A10, SLC39A13) were decreased (P ≤ 0.08), suggesting increased growth influences intracellular Zn trafficking and demands.

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Anabolic implants alter abundance of mRNA involved in muscle growth, metabolism, and inflammation in the longissimus of Angus steers in the feedlot

Cited by 2 publications

References 73 publications

Influence of steroidal implants and zinc sulfate supplementation on growth performance, trace mineral status, circulating metabolites, and transcriptional changes in skeletal muscle of feedlot steers

Influence of steroidal implants and zinc sulfate supplementation on growth performance, trace mineral status, circulating metabolites, and transcriptional changes in skeletal muscle of feedlot steers

Zinc supplementation and ractopamine hydrochloride impact gene expression of zinc transporters in finishing beef steers

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