Anaerobic bacteria from hypersaline environments.

Ollivier, Bernard; Cartigny, Pierre; Garcia, Jean‐Louis; Mah, Robert A.

doi:10.1128/mmbr.58.1.27-38.1994

Cited by 195 publications

(95 citation statements)

References 47 publications

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“…Sequences related to Firmicutes were abundant in layer N and related to the genera Halanaerobium and Bacillus. Halanaerobiales have been commonly found in other hypersaline environments (Ollivier et al, 1994;Rainey et al, 1995;Mouné et al, 1999), but an important part of the Firmicutes-related clones retrieved here formed a well-differentiated cluster outside Halanaerobiales and Clostridiales, and could be representatives of a new group of extremely halophilic grampositives (OPU HSB4, Fig. 3).…”

Section: Bacterial Clone Libraries Diversitymentioning

confidence: 75%

“…Hypersaline ecosystems are generally inhabited by a limited variety of life forms: invertebrates such as brine shrimp (Artemia salina) or brine flies, algae (Dunaliella salina), Bacteria and Archaea have been reported, and are the major sources of oxidizable compounds in these environments (Ollivier et al, 1994). In addition to organic matter originating from the dead cells and metabolites of the halophilic microbiota, algae and plants growing in the surroundings may introduce organic matter into the hypersaline system.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to organic matter originating from the dead cells and metabolites of the halophilic microbiota, algae and plants growing in the surroundings may introduce organic matter into the hypersaline system. Oxygen present in the overlying water column usually does not penetrate deeper than 2 mm into the sediment (Ollivier et al, 1994) and below such depths, oxygen is depleted as a consequence of both chemical combination with sulfide and consumption by different heterotrophic and chemotrophic organisms.…”

Section: Introductionmentioning

confidence: 99%

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Extremely halophilic microbial communities in anaerobic sediments from a solar saltern

López‐López

Yarza

Richter

et al. 2010

Environ Microbiol Rep

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The prokaryotic communities inhabiting hypersaline sediments underlying a crystallizer pond of a Mediterranean solar saltern have been studied in a polyphasic approach including 16S rRNA and dsrAB gene libraries analysis [the last encoding for dissimilatory (bi)sulfite reductase], most probable number of cultivable counts, and metabolic measurements of sulfate reduction. The samples studied here represent one of the most hypersaline anoxic environments sampled worldwide that harbour a highly diverse microbial community different from those previously reported in other hypersaline sediments. Both bacterial and archaeal types are present but, contrarily to the overlying brine system, the former dominates. Molecular analyses indicated that the bacterial fraction is highly diverse and mostly composed by groups related to sulfate-reducing bacteria (SRB). In good agreement with this, sulfate-reducing activity was detected in the sediment, as well as the metabolic diversity within SRB (as indicated by the use of different electron donors in enrichments). On the other hand, the archaeal fraction was phylogenetically homogeneous and, surprisingly, strongly affiliated with the MBSl-1 candidate division, an euryarchaeotal group only reported in deep-sea hypersaline anoxic basins of the Western Mediterranean, for which a methanogenic metabolism was hypothesized. The hypersaline studied samples constitute a valuable source of new prokaryotic types with metabolisms adapted to the prevalent in situ extreme conditions.

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Section: Bacterial Clone Libraries Diversitymentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Extremely halophilic microbial communities in anaerobic sediments from a solar saltern

López‐López

Yarza

Richter

et al. 2010

Environ Microbiol Rep

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“…The microorganism was found to survive salt concentrations of up to 10% and hence could be classified as a moderately halophilic isolate (Ollivier et al, 1994), which was an indication of its marine existence. Growth was observed only in Mannitol agar, and MPO failed to grow in MacConkey agar, thus suggesting its Gram-positive nature.…”

Section: Isolation and Identificationmentioning

confidence: 99%

Production of acylated homoserine lactone by Gram-positive bacteria isolated from marine water

Biswa

Doble

2013

FEMS Microbiol Lett

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Acylated homoserine lactone (AHL)-based quorum sensing (QS) has been reported to be present only in Gram-negative microorganisms. Isolation of a novel Gram-positive microorganism from sea water, capable of producing AHL, is reported here. The isolate (GenBank: JF915892, designated as MPO) belonging to the Exiguobacterium genera is capable of inducing the AHL bioreporters, namely Chromobacterium violaceum CV026, Agrobacterium tumefaceins A136, and E. coli JM 109(psb1075). This inducer is characterized as C3-oxo-octanoyl homoserine lactone (OOHL), and its production reaches a maximum of 15.6 μg L(-1), during the stationary growth phase of the organism. MPO extract when exogenously added inhibits the formation of biofilm for the same organism and lowers the extracellular polymeric substances, indicating an AHL-associated phenotypic trait. The isolated sequence of a probable LuxR homolog from MPO (designated as ExgR) shows similar functional domains and contains conserved residues in LuxR from other known bacterial QS LuxR regulators. Also present immediately downstream to ExgR was found a sequence showing homology to known LuxI synthase of Pseudomonas putida. qPCR analysis suggests an increment in exgR mRNA on addition of AHL, further proving the role of ExgR as a QS regulator.

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“…The dominance of methylotrophic methanogens may be particularly characteristic of hypersaline cyanobacterial mats, where rapid mineralization rates below the photic zone lead to high turnover of dead cells, and the subsequent release of their osmoregulatory solutes (King, 1988), such as glycine betaine, which are readily converted to methanogenic precursors, such as trimethylamine (King, 1984;Oren, 1990;1999;Ollivier et al, 1994). The clear increases in methane production following additions of methylated amines strongly suggest this to be the case in these mats, under normal sulfate concentrations.…”

Section: Methanogenesis From Non-competitive Substrates In Mats Undermentioning

confidence: 99%

Shifts in methanogen community structure and function associated with long‐term manipulation of sulfate and salinity in a hypersaline microbial mat

Smith

Green

Kelley

et al. 2007

Environmental Microbiology

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Methanogenesis was characterized in hypersaline microbial mats from Guerrero Negro, Baja California Sur, Mexico both in situ and after long-term manipulation in a greenhouse environment. Substrate addition experiments indicate methanogenesis to occur primarily through the catabolic demethylation of non-competitive substrates, under field conditions. However, evidence for the coexistence of other metabolic guilds of methanogens was obtained during a previous manipulation of sulfate concentrations. To fully characterize methanogenesis in these mats, in the absence of competition for reducing equivalents with sulfate-reducing microorganisms, we maintained microbial mats for longer than 1 year under conditions of lowered sulfate and salinity levels. The goal of this study was to assess whether observed differences in methane production during sulfate and salinity manipulation were accompanied by shifts in the composition of methanogen communities. Culture-independent techniques targeting methyl coenzyme M reductase genes (mcrA) were used to assess the dynamics of methanogen assemblages. Clone libraries from mats sampled in situ or maintained at field-like conditions in the greenhouse were exclusively composed of sequences related to methylotrophic members of the Methanosarcinales. Increases in pore water methane concentrations under conditions of low sulfate correlated with an observed increase in the abundance of putatively hydrogenotrophic mcrA, related to Methanomicrobiales. Geochemical and molecular data provide evidence of a significant shift in the metabolic pathway of methanogenesis from a methylotroph-dominated system in high-sulfate environments to a mixed community of methylotrophic and hydrogenotrophic methanogens under low sulfate conditions.

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Anaerobic bacteria from hypersaline environments.

Cited by 195 publications

References 47 publications

Extremely halophilic microbial communities in anaerobic sediments from a solar saltern

Extremely halophilic microbial communities in anaerobic sediments from a solar saltern

Production of acylated homoserine lactone by Gram-positive bacteria isolated from marine water

Shifts in methanogen community structure and function associated with long‐term manipulation of sulfate and salinity in a hypersaline microbial mat

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