Anaerobic benzene oxidation coupled to nitrate reduction in pure culture by two strains of Dechloromonas

Coates, John D.; Chakraborty, Romy; Lack, Joseph G.; O’Connor, Susan; Cole, Kimberly; Bender, Kelly S.; Achenbach, Laurie A.

doi:10.1038/35082545

Cited by 479 publications

(287 citation statements)

References 29 publications

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“…Furthermore, physiological and molecular characterization of anaerobic benzene-degrading, methanogenic and denitrifying enrichments showed the presence of diverse microbes, but, similar to the above reports, failed to identify the active microbes actually responsible for benzene degradation (Ulrich and Edwards, 2003). Nevertheless, to date several facultative anaerobic strains of the genera Dechloromonas and Azoarcus were isolated, which degraded benzene under denitrifying conditions (Coates et al, 2001b;Kasai et al, 2006). However, no strictly anaerobic microbe or microbial lineage was specifically identified to degrade benzene, despite the importance of these processes in contaminated groundwater environments.…”

Section: Introductionmentioning

confidence: 91%

The use of stable isotope probing to identify key iron-reducing microorganisms involved in anaerobic benzene degradation

2007

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Here, we present a detailed functional and phylogenetic characterization of an iron-reducing enrichment culture maintained in our lab with benzene as sole carbon and energy source. We used DNA-stable isotope probing to identify microbes within the enrichment most active in the assimilation of 13 C-label. When 12 C 6 -and 13 C 6 -benzene were added as comparative substrates, marked differences in the quantitative buoyant density distribution became apparent especially for uncultured microbes within the Gram-positive Peptococcaceae, closely related to environmental clones retrieved from contaminated aquifers world wide and only distantly related to cultured representatives of the genus Thermincola. Prominent among the other constituents of the enrichment were uncultured Deltaproteobacteria, as well as members of the Actinobacteria. Although their presence within the enrichment seems to be stable they did not assimilate 13 C-label as significantly as the Clostridia within the time course of our experiment. We hypothesize that benzene degradation in our enrichment involves an unusual syntrophy, where members of the Clostridia primarily oxidize benzene. Electrons from the contaminant are both directly transferred to ferric iron by the primary oxidizers, but also partially shared with the Desulfobulbaceae as syntrophic partners. Alternatively, electrons may also be quantitatively transferred to the partners, which then reduce the ferric iron. Thus our results provide evidence for the importance of a novel clade of Gram-positive iron-reducers in anaerobic benzene degradation, and a role of syntrophic interactions in this process. These findings shed a totally new light on the factors controlling benzene degradation in anaerobic contaminated environments.

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Section: Introductionmentioning

confidence: 91%

The use of stable isotope probing to identify key iron-reducing microorganisms involved in anaerobic benzene degradation

2007

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“…Methanogens are strict anaerobes. According to Coates et al, contaminating petroleum can prevent ventilation to soil and stimulate the oxygen consumption, resulting in the formation of anaerobic zones (27). It is possible that anaerobic zones suitable for growth of methanogens are present in the contaminated soil due to the serious oil pollution (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Comparison of archaeal and bacterial community structures in heavily oil-contaminated and pristine soils

Liu

Zhang

Ding

et al. 2009

Journal of Bioscience and Bioengineering

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Archaeal and bacterial community structures in heavily oil-contaminated and pristine soils were compared using denaturing gradient gel electrophoresis and 16S rRNA gene libraries. The results showed that archaeal diversity was more complex in the contaminated soil than in the uncontaminated control soil. Archaeal populations in the contaminated soil consisted mainly of Euryarchaeota, with abundant methanogen-like operational taxonomic units (OTUs) and OTUs related to the phylogenetically diverse group, candidate division I, corresponding to rice cluster V. In contrast, only halophilic archaea were found in the pristine soil. Bacterial community structures also differed significantly between the contaminated and pristine soils. More clones from the contaminated soil were related to known hydrocarbon-degrading bacteria, implying that microorganisms with the potential to degrade petroleum were well-established. These results provide further insights into the composition of microbial communities in oil-contaminated soils.

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“…(ACD23) (Coates et al, 2001;Byrne-Bailey et al, 2010). Organisms closely related to those studied here have been demonstrated to have a role in nitrate, as well as selenium reduction at this and other subsurface sites (Byrne-Bailey et al, 2010).…”

Section: Metabolic Interdependencies In An Aquifer Microbial Communitmentioning

confidence: 99%

Metabolic interdependencies between phylogenetically novel fermenters and respiratory organisms in an unconfined aquifer

et al. 2014

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Fermentation-based metabolism is an important ecosystem function often associated with environments rich in organic carbon, such as wetlands, sewage sludge and the mammalian gut. The diversity of microorganisms and pathways involved in carbon and hydrogen cycling in sediments and aquifers and the impacts of these processes on other biogeochemical cycles remain poorly understood. Here we used metagenomics and proteomics to characterize microbial communities sampled from an aquifer adjacent to the Colorado River at Rifle, CO, USA, and document interlinked microbial roles in geochemical cycling. The organic carbon content in the aquifer was elevated via acetate amendment of the groundwater occurring over 2 successive years. Samples were collected at three time points, with the objective of extensive genome recovery to enable metabolic reconstruction of the community. Fermentative community members include organisms from a new phylum, Melainabacteria, most closely related to Cyanobacteria, phylogenetically novel members of the Chloroflexi and Bacteroidales, as well as candidate phyla genomes (OD1, BD1-5, SR1, WWE3, ACD58, TM6, PER and OP11). These organisms have the capacity to produce hydrogen, acetate, formate, ethanol, butyrate and lactate, activities supported by proteomic data. The diversity and expression of hydrogenases suggests the importance of hydrogen metabolism in the subsurface. Our proteogenomic data further indicate the consumption of fermentation intermediates by Proteobacteria can be coupled to nitrate, sulfate and iron reduction. Thus, fermentation carried out by previously unknown members of sediment microbial communities may be an important driver of nitrogen, hydrogen, sulfur, carbon and iron cycling.

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Anaerobic benzene oxidation coupled to nitrate reduction in pure culture by two strains of Dechloromonas

Cited by 479 publications

References 29 publications

The use of stable isotope probing to identify key iron-reducing microorganisms involved in anaerobic benzene degradation

The use of stable isotope probing to identify key iron-reducing microorganisms involved in anaerobic benzene degradation

Comparison of archaeal and bacterial community structures in heavily oil-contaminated and pristine soils

Metabolic interdependencies between phylogenetically novel fermenters and respiratory organisms in an unconfined aquifer

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