Anaerobic Biodegradation of Hydrocarbons Including Methane

Widdel, Friedrich; Boetius, Antje; Rabus, Ralf

doi:10.1007/0-387-30742-7_33

Cited by 95 publications

(70 citation statements)

References 138 publications

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“…Cultures were incubated at 20 uC in HCO 2 3 /CO2-buffered full marine mineral medium, supplemented with vitamins and trace elements as described by Widdel et al (2004) with minor modifications to accommodate the needs of denitrifiers: 100 mg MnCl 2 . 4H 2 O l -1 and 29 mg CuCl 2 .…”

Section: Methodsmentioning

confidence: 99%

“…Anoxic conditions in enrichments were achieved solely by degassing and flushing with N 2 / CO 2 (90 : 10, v/v). In pure cultures, 0.5 mM sodium sulphide or 4 mM freshly prepared sodium ascorbate was used in addition to establish reducing conditions (Widdel et al, 2004). Ascorbate did not serve as a growth substrate for the isolated strains.…”

Section: Methodsmentioning

confidence: 99%

“…Ascorbate did not serve as a growth substrate for the isolated strains. Toluene and nhexane were prepared as described elsewhere (Ehrenreich et al, 2000;Widdel et al, 2004) and resupplied when consumed. Enrichment cultures were performed in butyl-rubber-stopper-sealed 250 ml flat glass bottles containing 8 ml homogenized sediments, 150 ml mineral medium and 16 ml of the substrate-containing carrier phase, under a headspace of N 2 /CO 2 (90 : 10, v/v).…”

Section: Methodsmentioning

confidence: 99%

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Anaerobic utilization of toluene by marine alpha- and gammaproteobacteria reducing nitrate

et al. 2012

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Aromatic hydrocarbons are among the main constituents of crude oil and represent a major fraction of biogenic hydrocarbons. Anthropogenic influences as well as biological production lead to exposure and accumulation of these toxic chemicals in the water column and sediment of marine environments. The ability to degrade these compounds in situ has been demonstrated for oxygen-and sulphate-respiring marine micro-organisms. However, if and to what extent nitratereducing bacteria contribute to the degradation of hydrocarbons in the marine environment and if these organisms are similar to their well-studied freshwater counterparts has not been investigated thoroughly. Here we determine the potential of marine prokaryotes from different sediments of the Atlantic Ocean and Mediterranean Sea to couple nitrate reduction to the oxidation of aromatic hydrocarbons. Nitrate-dependent oxidation of toluene as an electron donor in anoxic enrichment cultures was elucidated by analyses of nitrate, nitrite and dinitrogen gas, accompanied by cell proliferation. The metabolically active members of the enriched communities were identified by RT-PCR of their 16S rRNA genes and subsequently quantified by fluorescence in situ hybridization. In all cases, toluene-grown communities were dominated by members of the Gammaproteobacteria, followed in some enrichments by metabolically active alphaproteobacteria as well as members of the Bacteroidetes. From these enrichments, two novel denitrifying toluenedegrading strains belonging to the Gammaproteobacteria were isolated. Two additional toluenedegrading denitrifying strains were isolated from sediments from the Black Sea and the North Sea. These isolates belonged to the Alphaproteobacteria and Gammaproteobacteria. Serial dilutions series with marine sediments indicated that up to 2.2¾10 4 cells cm -3 were able to degrade hydrocarbons with nitrate as the electron acceptor. These results demonstrated the hitherto unrecognized capacity of alpha-and gammaproteobacteria in marine sediments to oxidize toluene using nitrate.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Anaerobic utilization of toluene by marine alpha- and gammaproteobacteria reducing nitrate

et al. 2012

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“…The distinctive and flexible mechanism of respiration, using nitrate as the terminal electron acceptor in lieu of oxygen can be exploited efficiently in the degradation of hydrocarbons under mixed aerobic / microaerophilic conditions [10]. Biodegradation of hydrocarbons, especially the aromatics by indigenous strains of hydrocarbon-degrading denitrifying bacteria, therefore represents an efficient approach towards pollution remediation [5,[11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Implications of Benzoate Induced Alterations in Cell Morphology and Physiology in Pseudomonas aeruginosa TMR2.13 for Potential Application in Bioremediation and Monitoring Approaches

Sousa¹

2011

J Bioremed Biodegrad

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“…To confirm these results and to test for the capacity of the strain to catabolize different substrates as sole carbon and energy sources with oxygen as a terminal electron acceptor, the strain was grown aerobically, in the dark, on a mineral medium supplemented with one substrate. The defined medium (modified from Widdel et al, 2006) had the following composition (l 21 ): phosphate buffer, 30 mM; NaCl, 20 g; MgCl 2 . 6H 2 O, 3 g; CaCl 2 .…”

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confidence: 99%

Hellea balneolensis gen. nov., sp. nov., a prosthecate alphaproteobacterium from the Mediterranean Sea

Alain

Tindall

Intertaglia

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A novel aerobic, heterotrophic, prosthecate bacterium, designated 26III/A02/215 T , was isolated from surface water of the north-western Mediterranean Sea. Cells stained Gram-negative and were straight to slightly curved rods, forming red colonies on agar plates. The strain grew at 15-37 6C inclusive (optimum 30 6C) and grew optimally at seawater salinity. Growth was observed on organic acids, amino acids and complex organic substrates. The fatty acids (.5 %) detected in strain 26III/A02/215 T were C 17 : 1 v6c, C 18 : 1 v7c and C 17 : 0 . The lipid pattern indicated the presence of phosphatidylglycerol, glucuronopyranosyldiglyceride, monoglycosyldiglyceride, an unidentified glycolipid and three unidentified phospholipids. Phosphatidylethanolamine and diphosphatidylglycerol were absent. Ubiquinone Q-10 was the only respiratory lipoquinone. The G+C content of the genomic DNA was 46.8 mol%. Comparative 16S rRNA gene sequence analysis indicated that strain 26III/A02/215 T belonged to the Hyphomonas-HirschiaRobiginitomaculum branch of the order Caulobacterales. This affiliation was consistent with the results of polar lipid analyses. Among this group, the novel isolate was most closely related to Robiginitomaculum antarcticum (93.9 % 16S rRNA gene sequence similarity to the type strain). On the basis of genotypic, chemotaxonomic and phenotypic distinctness, we propose a novel genus, Hellea gen. nov., with Hellea balneolensis sp. nov. as the type species. The type strain of Hellea balneolensis is 26III/A02/215 T (5DSM 19091 T 5CIP 109500 T 5OOB 269 T ).The phylum Proteobacteria is one of the 24 phyla of the domain Bacteria described in Bergey's Manual of Systematic Bacteriology, 2nd edn (Garrity & Holt, 2001). To date, more than 200 genera have been described, making this one of the largest bacterial phyla. Members of this group are widely distributed in nature and are physiologically and metabolically diverse. The phylum Proteobacteria is currently divided into five classes, the Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Deltaproteobacteria and Epsilonproteobacteria, all of which have been defined exclusively on the basis of 16S rRNA gene sequence analysis (Garrity & Holt, 2001). At present, the class (Yabuuchi & Kosako, 2005). Marine species make up more than half of the species of the Alphaproteobacteria described to date. Some confusion is being caused at present by the different taxonomic placement of the 'stalked' bacteria. While Lee et al. (2005) place the members of the genera Hyphomonas, Oceanicaulis, Hirschia and Maricaulis in a new family, the Hyphomonadaceae, within the order Caulobacterales (which includes members of the family Rhodobacteraceae), Garrity et al. (2005a) have placed members of these genera within the family Rhodobacteraceae, within the order Rhodobacterales, leaving the members of the family Caulobacteraceae within the order Caulobacterales. This situation is particularly unsatisfactory since use of the Abbreviation: TBSA, tuberculostearic acid. The GenBank/EMBL/DDBJ ac...

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Anaerobic Biodegradation of Hydrocarbons Including Methane

Cited by 95 publications

References 138 publications

Anaerobic utilization of toluene by marine alpha- and gammaproteobacteria reducing nitrate

Anaerobic utilization of toluene by marine alpha- and gammaproteobacteria reducing nitrate

Implications of Benzoate Induced Alterations in Cell Morphology and Physiology in Pseudomonas aeruginosa TMR2.13 for Potential Application in Bioremediation and Monitoring Approaches

Hellea balneolensis gen. nov., sp. nov., a prosthecate alphaproteobacterium from the Mediterranean Sea

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