Anaerobic biodegradation of polycyclic aromatic hydrocarbon in soil

Chang, Bea‐Ven; Shiung, Lau-Lun; Yuan, S.Y.

doi:10.1016/s0045-6535(02)00151-0

Cited by 180 publications

(78 citation statements)

References 18 publications

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“…Consequently, persistence to in situ biodegradation in absence of O 2 was proposed. In contrast this conclusion based on field experiments but in agreement with the clear increase in 13 C-CO 2 in our microcosm experiments, enrichment cultures served to demonstrate biodegradation of small concentrations of acenaphthene under denitrifying, sulfate-reducing, and methanogenic conditions, after incubation times longer than 80 days, (Chang et al, 2002;Mihelcic and Luthy, 1988).…”

Section: Intrinsic Biodegradation Potential Under Oxic and Anoxic Consupporting

confidence: 89%

Evidence for in situ degradation of mono-and polyaromatic hydrocarbons in alluvial sediments based on microcosm experiments with 13C-labeled contaminants

Morasch

Höhener

Hunkeler

2007

Environmental Pollution

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A method based on 13 C-labeled substrates was developed to determine the intrinsic biodegradation potential of aromatic pollutants under oxic and under anoxic conditions. AbstractA microcosm study was conducted to investigate the degradation of mono-and polyaromatic hydrocarbons under in situ-like conditions using alluvial sediments from the site of a former cokery. Benzene, naphthalene, or acenaphthene were added to the sediments as 13 C-labeled substrates. Based on the evolution of 13 C-CO 2 determined by gas chromatography isotope-ratio mass spectrometry (GC-IRMS) it was possible to prove mineralization of the compound of interest in the presence of other unknown organic substances of the sediment material. This new approach was suitable to give evidence for the intrinsic biodegradation of benzene, naphthalene, and acenaphthene under oxic and also under anoxic conditions, due to the high sensitivity and reproducibility of 13 C/ 12 C stable isotope analysis. This semi-quantitative method can be used to screen for biodegradation of any slowly degrading, strongly sorbing compound in long-term experiments.

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Section: Intrinsic Biodegradation Potential Under Oxic and Anoxic Consupporting

confidence: 89%

Evidence for in situ degradation of mono-and polyaromatic hydrocarbons in alluvial sediments based on microcosm experiments with 13C-labeled contaminants

Morasch

Höhener

Hunkeler

2007

Environmental Pollution

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“…The occurrence of PAHs in anaerobic conditions depends on certain factors which include substrate interaction, pH and redox conditions (Chang, Shiung, & Yuan, 2002). Stimulation of PAHs degradation rate under sulphate reducing conditions have been examined (Bach, Kim, Choi, & Oh, 2005).…”

Section: Chemical Degradationmentioning

confidence: 99%

Polycyclic Aromatic Hydrocarbons Degradation Techniques: A Review

Ukiwe¹,

Egereonu²,

Njoku³

et al. 2013

IJC

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The demand for processed petroleum products and agricultural produce has exposed our environment to polycyclic aromatic hydrocarbons (PAHs) contamination. PAHs stick to solid sediments and are ubiquitous including soil, water and air. Their presence in these media creates problems because consuming products obtained from these sources could be deleterious to human health since several of these compounds (benz(a)anthracene, benzo(a)pyrene, chrysene etc) have been implicated in causing tumors in animals and cancer in humans. The present review describes several remediation techniques which are efficient and cost effective in removing PAHs from the environment. Some of these conventional clean-up methods are not only environmental friendly; they also present a novel approach in reducing the ability of PAHs to cause prospective risk to humans and the ecosystem.

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“…Previous studies have demonstrated that the addition of carbon sources (such as acetate, pyruvate, and lactate) could enhance PAHs (acenaphthene, fluorine, and phenanthtene) degradation by two to three times in contaminated soils (Chang et al 2002;Chang et al 2008). The proposed explanation was that (n=3) these carbon sources promoted the growth of particular consortium and the production of more electrons to accelerate the process (Chang et al 2002;Chang et al 2008). For pure cultures, Ye et al (1995) found that benzo (a)pyrene mineralization by Sphingomonas paucimobilis strain EPA 505 (an aerobic strain) was increased from 5% to 28% when glucose was amended in the liquid culture.…”

Section: Anaerobic Degradation Of Phenanthrenementioning

confidence: 99%

Anaerobic degradation of phenanthrene by a newly isolated humus-reducing bacterium, Pseudomonas aeruginosa strain PAH-1

Wang

et al. 2011

J Soils Sediments

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Purpose The anaerobic degradation of polycyclic aromatic hydrocarbons (PAHs) has great significance to PAHs' natural attenuation in contaminated sites. Previous studies mainly focused on anaerobic PAH degradation by mixed cultures with nitrate, sulfate, or Fe(III) oxides as electron acceptors, and the roles of pure cultures in the process was rarely reported. The aim of this paper is to isolate a pure culture that is capable of degrading phenanthrene anaerobically with anthraquinone-2,6-disulphonate (AQDS) as the sole electron acceptor and evaluate its environmental functions. Materials and methods A strain of pure culture was isolated from anodic solution of a microbial fuel cell via enrichment procedure with phenanthrene and AQDS under anaerobic conditions. Using the single colony isolation technique, a distinct colony was obtained and identified by the phenotypic and phylogenetic analysis. Its ability to reduce AQDS and degrade phenanthrene was conducted in serum bottles by standard anaerobic techniques (purged with 80% N 2 −20% CO 2 ). The concentration of AH 2 QDS and fructose was quantified by UV-vis spectrophotometer at 450 and 210 nm, respectively. Cells number was determined by direct plate counting on aerobic LB agar medium. The concentration of phenanthrene was determined using HPLC. Results and discussion Strain PAH-1 was identified as Pseudomonas aeruginosa. It could oxidize fructose or glucose to reduce AQDS and can support microbial growth by conserving energy from fructose to AQDS. It has the ability to degrade phenanthrene directly with AQDS as the sole electron acceptor (46.5% removal), and the microbial process may be AQDS dependent. The addition of small organic substances (fructose) could enhance the anaerobic biodegradation of phenanthrene from 46.5% to 56.7%. The anaerobic degradation of phenanthrene fits the pseudo-firstorder kinetics, giving the rate constants of 0.0233/day (R 2 =0.934) and 0.0328/day (R 2 =0.933) for non-fructose set and fructose set, respectively. Conclusions We successfully isolated a facultative anaerobe, P. aeruginosa strain PAH-1. This study was the first paper reporting that a pure culture (strain PAH-1) has the ability to anaerobically degrade phenanthrene with AQDS as the sole electron acceptor. The finding also explores the environmental significance of the Pseudomonas genus.

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Anaerobic biodegradation of polycyclic aromatic hydrocarbon in soil

Cited by 180 publications

References 18 publications

Evidence for in situ degradation of mono-and polyaromatic hydrocarbons in alluvial sediments based on microcosm experiments with 13C-labeled contaminants

Evidence for in situ degradation of mono-and polyaromatic hydrocarbons in alluvial sediments based on microcosm experiments with 13C-labeled contaminants

Polycyclic Aromatic Hydrocarbons Degradation Techniques: A Review

Anaerobic degradation of phenanthrene by a newly isolated humus-reducing bacterium, Pseudomonas aeruginosa strain PAH-1

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