About 70 to 80% of the coffee berries husk end up as agricultural waste after processing. Its neglect or improper utility can cause environmental pollution. The present study investigated the efficiency of Streptomyces, Trichoderma harzianum and the combination of both as bio-degradable agents of coffee husk which is regarded as agricultural waste. Identification of their efficiency is necessary to stimulate further research on their probable utility in converting huge mass of coffee husk to organic manure for soil amendment and higher crop productivity. Coffee husk from the coffee processing unit of the Cocoa Research Institute of Nigeria (CRIN), Ibadan was the substrate for the isolates of Streptomyces, Trichoderma and Streptomyces/Trichoderma. Culturing was observed for 0, 15 and 30 days. The factorial in completely randomized design experiment had ten treatment combinations (control inclusive) of three replications. Significant (P < 0.001) variation existed among the three periodic days for all the generated biochemical products. The three organisms and the interaction between organisms and days differed significantly (P ≤ 0.05) for moisture content, dry matter content, phosphorus content and the pH. The production trend of most of the biochemical as aided by Streptomyces was both linear and quadratic for the three days. Caffeine, tannin, phosphorus, potassium and dry matter content were significantly highest in the control. Wricke ecovalence was least for the production of moisture content, phosphorus, dry matter, tannin and pH in the treatment which combines the two organisms. For the production of biochemical from the incubated substrate, the nine treatments significantly differed from the control denoting that microorganisms are needed in organic matter decomposition process.