Anaerobic degradation of 2-chloro-4-nitroaniline by <i>Geobacter</i> sp. KT7 and <i>Thauera aromatica</i> KT9

Duc, Ha Danh

doi:10.1093/femsle/fnz174

Cited by 14 publications

(6 citation statements)

References 40 publications

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“…KT7 and Thauera aromatica KT9 cooperatively degraded 2-chloro-4-nitroaniline with an increased degradation rate compared to each species in isolation. 60 It was suggested that cross-feeding and nutrient sharing between the two species led to increased degradation. However, it has not been elucidated which enzymes contributed to the reduction of 2-chloro-4-nitroaniline.…”

Section: Discussionmentioning

confidence: 99%

Bacteria Make a Living Breathing the Nitroheterocyclic Insensitive Munitions Compound 3-Nitro-1,2,4-triazol-5-one (NTO)

Madeira

Menezes

Park

et al. 2021

Environ. Sci. Technol.

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The nitroheterocyclic 3-nitro-1,2,4-triazol-5-one (NTO) is an ingredient of insensitive explosives increasingly used by the military, becoming an emergent environmental pollutant. Cometabolic biotransformation of NTO occurs in mixed microbial cultures in soils and sludges with excess electron-donating substrates. Herein, we present the unusual energy-yielding metabolic process of NTO respiration, in which the NTO reduction to 3-amino-1,2,4-triazol-5-one (ATO) is linked to the anoxic acetate oxidation to CO2 by a culture enriched from municipal anaerobic digester sludge. Cell growth was observed simultaneously with NTO reduction, whereas the culture was unable to grow in the presence of acetate only. Extremely low concentrations (0.06 mg L–1) of the uncoupler carbonyl cyanide m-chlorophenyl hydrazone inhibited NTO reduction, indicating that the process was linked to respiration. The ultimate evidence of NTO respiration was adenosine triphosphate production due to simultaneous exposure to NTO and acetate. Metagenome sequencing revealed that the main microorganisms (and relative abundances) were Geobacter anodireducens (89.3%) and Thauera sp. (5.5%). This study is the first description of a nitroheterocyclic compound being reduced by anaerobic respiration, shedding light on creative microbial processes that enable bacteria to make a living reducing NTO.

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Section: Discussionmentioning

confidence: 99%

Bacteria Make a Living Breathing the Nitroheterocyclic Insensitive Munitions Compound 3-Nitro-1,2,4-triazol-5-one (NTO)

Madeira

Menezes

Park

et al. 2021

Environ. Sci. Technol.

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“…These results indicate a synergistic activity in the degradation of the parent compound and its metabolites by the microbial community in slurries. Previous studies have also indicated that the anaerobic degradation of complex organic compounds is mainly carried out by mixed cultures (Ghattas et al 2017;Duc 2019). The cooperation between microbial populations to degrade diuron and other metabolites in soil under aerobic conditions has also been described (Villaverde et al 2017).…”

Section: Identi Cation Of Metabolites Produced By Diuron Degradationmentioning

confidence: 99%

“…Native bacteria in sediment and soil bacteria had adapted to speci c conditions and exhibited synergistic interactions for herbicide degradation. The compatible bacterial strains can enhance the degradation of organic compounds (Duc 2019;Oanh and Duc 2021).…”

Section: Identi Cation Of Metabolites Produced By Diuron Degradationmentioning

confidence: 99%

Composition of bacterial community and isolation of bacteria responsible for diuron degradation in sediment and soil under anaerobic condition

Duc

Oanh

2022

Arch Microbiol

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The herbicide diuron is extensively used in the agriculture sector and is detected widely in the environment. Although several studies on the degradation of diuron by aerobic micro-organisms have been reported, the degradation of diuron by anaerobic micro-organisms has not been received much attention. Also, no pure culture that can degrade diuron under anaerobic conditions has yet been reported.The evaluation of diuron degradation in the soil and sediment slurries showed that diuron led to a decrease in the biodiversity of the bacterial communities. Two mixed bacterial cultures, one from the soil and the other from sediment slurries, were isolated from the enrichment media under anaerobic conditions. After 30 days of incubation at 30 o C, the mixed bacterial culture from the soil degraded 84.5 ± 5.5%, and that from the sediment slurry degraded 94.5 ± 3.0% of diuron in liquid mineral medium at an initial concentration of 20 mg/L. 1-(3,4-dichlorophenylurea (DCPU), 3-(3-chlorophenyl)-1,1-dimethylurea (CPDMU), and 3,4-dichloroaniline (3,4-DCA) were the major diuron metabolites produced by both the indigenous micro-organisms and the isolated bacteria.

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“…One product was formed after chlorination and long time storage. Remarkably, 4-nitroaniline was the major product formed for all the degradation processes and also the most mutagenic product (Franco et al, 2018); (Duc, 2019). That is, in a water treatment plant, the dye would probably not be detected, because sunlight and chlorination promote its degradation drastically.…”

Section: Detection Of Ab210 and Degradation Products By Lc-ms/msmentioning

confidence: 99%

Stability of Acid Black 210 dye in Tannery Industry Effluent in Aqueous Solution Is Limited and Generates Harmful Subproducts

Christovam

Franco

Zocolo

et al. 2022

Front. Environ. Sci.

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The present work investigates the occurrence of the Acid Black 210 (AB210) dye and its subproducts in the tannery industry by analytical techniques. The AB210 is an important dye characterized by three azo groups as a chromophore and is one of the most used azo dyes in the tannery industry. The stability of AB210 in front of chlorination, sunlight exposition, and ambient conditions was investigated, as well as its occurrence and degradation products in the tannery wastewater. The stability study of AB210 showed a decrease in dye concentration of up to 45% after 14 days at room temperature. The exposure of the AB 210 by a solar simulator for 3 h showed discoloration of the dye. Furthermore, the chlorination of the AB210 caused a reduction of 25% in the intensity of the absorption band at the visible region after 300 s of treatment with sodium hypochlorite (NaClO). Studies based on high-performance liquid chromatography (HPLC-DAD), liquid chromatography-mass spectrometry (LC-MS/MS), and nuclear magnetic resonance (NMR) have indicated the occurrence of several harmful compounds such as benzene, cresol, naphthalene, phenol, 2-naphthylamine, and phenylacetic acid, and three aromatic amines, 2-naphthylamine, 2,6-dimethylaniline and 4-nitroaniline from the tannery industry. The cytotoxicity assay showed toxicity for the samples stored for a long period. Thus, the immortalized human keratinocyte (HaCAT) and 3T3 cells assays for the AB210 stored for 14 days showed 70% cell death in both strains evaluated. Our results demonstrated that the AB210 degradation is a great environmental concern due to increased toxicity for the body of living beings, especially for humans, as their biotransformation produces harmful compounds such as amines, which have been widely condemned by the International Agency for Research on Cancer.

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Anaerobic degradation of 2-chloro-4-nitroaniline by Geobacter sp. KT7 and Thauera aromatica KT9

Cited by 14 publications

References 40 publications

Bacteria Make a Living Breathing the Nitroheterocyclic Insensitive Munitions Compound 3-Nitro-1,2,4-triazol-5-one (NTO)

Bacteria Make a Living Breathing the Nitroheterocyclic Insensitive Munitions Compound 3-Nitro-1,2,4-triazol-5-one (NTO)

Composition of bacterial community and isolation of bacteria responsible for diuron degradation in sediment and soil under anaerobic condition

Stability of Acid Black 210 dye in Tannery Industry Effluent in Aqueous Solution Is Limited and Generates Harmful Subproducts

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