Anaerobic degradation of phenol by pure cultures of newly isolated denitrifying pseudomonads

Tschech, Andreas; Fuchs, Georg

doi:10.1007/bf00414814

Cited by 273 publications

(210 citation statements)

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“…The medium was supplemented with 1 mL/L trace element solution (27) and adjusted to pH 7.2 before sterilization by autoclaving. Afterward, 0.1 mL/L sterile vitamin solution (28) was added. The cultures were kept in 250-mL glass bottles, which contained 185 mL of medium and were closed with Mininert-Valves (Vici Precision Sampling, Baton Rouge, LA).…”

Section: Methodsmentioning

confidence: 99%

Hydrogen and Carbon Isotope Fractionation during Aerobic Biodegradation of Benzene

Hunkeler

Andersen

Aravena

et al. 2001

Environ. Sci. Technol.

165

112

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The main aim of the study was to evaluate hydrogen and carbon isotope fractionation during biodegradation of benzene as a possible tool to trace the process in contaminated environments. Aerobic biodegradation of benzene by two bacterial isolates, Acinetobacter sp. and Burkholderia sp., was accompanied by significant hydrogen and carbon isotope fractionation with hydrogen isotope enrichment factors of -12.8 ( 0.7‰ and -11.2 ( 1.8‰, respectively, and average carbon isotope enrichment factors of -1.46 ( 0.06‰ and -3.53 ( 0.26‰, respectively. Inorganic carbon produced by Acinetobacter sp. was depleted in 13 C by 3.6-6.2‰ as compared to the initial δ 13 C of benzene, while the produced biomass was enriched in 13 C by 3.8‰. The secondary aim was to determine isotope ratios of benzenes from different manufacturers with regard to the use of isotopes for source differentiation. While two of the four analyzed benzenes had similar δ 13 C values, each of them had a distinct δ 2 H-δ 13 C pair and δ 2 H values spread over a range of 66.5‰. Thus, combined analyses of hydrogen and carbon isotopes may be a more promising approach to trace sources and/or biodegradation of benzene than measuring carbon isotopes only.

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Section: Methodsmentioning

confidence: 99%

Hydrogen and Carbon Isotope Fractionation during Aerobic Biodegradation of Benzene

Hunkeler

Andersen

Aravena

et al. 2001

Environ. Sci. Technol.

165

112

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“…Bacterial cells were harvested by centrifugation at 16000 g for 10 min, washed twice in 0-1 5 M saline/O-lO M EDTA pH 8-0 and, if necessary, stored at -20 "C. DNA was prepared and DNA-DNA hybridization was done by the thermal renaturation rate method of De Ley et al (1 970) using a Gilford model 2600 spectrophotometer as described previously (Wiese et al, 1996). ?Data from Anders et al (1995), Tschech &Fuchs (1987) andSong et al (1998). f Data from Macy (1992) andMacy et al (1993).…”

Section: Methodsmentioning

confidence: 99%

Thauera mechernichensis sp. nov., an aerobic denitrifier from a leachate treatment plant

Scholten

Lukow

Auling

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

140

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A heterotrophic bacterial strain TLIT capable of aerobic denitrification was previously enriched in continuous culture from a landfill leachate treatment plant and isolated as a pure culture. The taxonomic position of this isolate within the B-subclass of the Proteobacteria was determined by 16s rDNA sequence analysis and by conventional taxonomy including substrate spectrum, quinone type (ubiquinone 4-8) and cellular fatty acid composition.Detection of the specific polyamine 2-hydroxyputrescine supports the membership of strain TLIT in the B-subclass of the Proteobacteria. The results of 165 rDNA sequencing showed that the strain clustered with, but was separate from, Thauera aromatica and Thauera selenatis. DNA-DNA hybridization experiments indicated that the new isolate represents a new species of the genus, for which the name Thauera mechernichensis is proposed; the type strain is DSM 12266T.

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“…Pseudomonas K 172 was grown anaerobically on mineral salt medium at 28 "C, with phenol plus C 0 2 or with 4-hydroxybenzoate as carbon sources and with nitrate as electron acceptor [3]. Growth was followed by determining the apparent increase in absorbance at 578 nm, corrected for the absorbance of the medium.…”

Section: Bacterial Growthmentioning

confidence: 99%

“…In addition phenol is one of the most prominent ground water contaminants. The aerobic metabolism of phenol has been studied extensively; as in all pathways of aerobic aromatic metabolism, oxygenases initiate the degradation of phenol [2].Evidence has recently been presented that phenol is metabolized to C 0 2 by pure bacterial cultures in the absence of molecular oxygen [3] (Fig. …”

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Catalytic properties of phenol carboxylase In vitro study of CO₂: 4‐hydroxybenzoate isotope exchange reaction

Lack

Tommasi

Aresta

et al. 1991

European Journal of Biochemistry

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Phenol is metabolized in a denitrifying bacterium in the absence of molecular oxygen via para-carboxylation to 4-hydroxybenzoate (biological Kolbe-Schmitt synthesis). The enzyme system catalyzing the presumptive carboxylation of phenol, tentatively named 'phenol carboxylase', catalyzes an isotope exchange between 14C02 and the carboxyl group of 4-hydroxybenzoate (specific activity 0.1 pmol 14C02 incorporated into 4-hydroxybenzoate x min-x mg-cell protein) which is considered a partial reaction of the overall enzyme catalysis; 14C from [14C]phenol was not exchanged into 4-hydroxybenzoate ring positions to a significant extent. The 14C02 isotope exchange reaction was studied in vitro. The reaction was dependent on the substrates C 0 2 and 4-hydroxybenzoate and required K + and Mn2+. The actual substrate was C 0 2 rather than HCO,. The apparent K,,, values were 1 mM dissolved COz, 0.2 mM 4-hydroxybenzoate, 2 mM K', and 0.1 mM MnZ+. The cationic cocatalysts could be substituted by ions of similar ionic radius: K' could be replaced to some extent by Rb', but not by Li+, Na', Cs+, or NH:; Mn2+ could be replaced to some extent by Fe2+ >Mg2+, Co2', but not by Ni2+, Zn2+, CaZ+, or Cu2+. The exchange reaction was not strictly specific for 4-hydroxybenzoate, however it required a p-hydroxyl group : derivatives of 4-hydroxybenzoate with OH, CH3 or C1 substituents in m-position did react, whereas those with substitutions in the o-position were inactive or were inhibitory. The enzyme was induced when cells were grown on phenol, but not on 4-hydroxybenzoate. Comparison of SDSjPAGE protein patterns of cells grown on phenol or 4-hydroxybenzoate revealed several additional protein bands in phenolgrown cells. The possible role of similar enzymes in the anaerobic metabolism of phenolic compounds is discussed.Phenolic compounds are important plant constituents and phenol is formed by the activity of microorganisms from a variety of natural and synthetic substrates [l]. In addition phenol is one of the most prominent ground water contaminants. The aerobic metabolism of phenol has been studied extensively; as in all pathways of aerobic aromatic metabolism, oxygenases initiate the degradation of phenol [2].Evidence has recently been presented that phenol is metabolized to C 0 2 by pure bacterial cultures in the absence of molecular oxygen [3] (Fig.

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Anaerobic degradation of phenol by pure cultures of newly isolated denitrifying pseudomonads

Cited by 273 publications

References 21 publications

Hydrogen and Carbon Isotope Fractionation during Aerobic Biodegradation of Benzene

Hydrogen and Carbon Isotope Fractionation during Aerobic Biodegradation of Benzene

Thauera mechernichensis sp. nov., an aerobic denitrifier from a leachate treatment plant

Catalytic properties of phenol carboxylase In vitro study of CO₂: 4‐hydroxybenzoate isotope exchange reaction

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Anaerobic degradation of phenol by pure cultures of newly isolated denitrifying pseudomonads

Cited by 273 publications

References 21 publications

Hydrogen and Carbon Isotope Fractionation during Aerobic Biodegradation of Benzene

Hydrogen and Carbon Isotope Fractionation during Aerobic Biodegradation of Benzene

Thauera mechernichensis sp. nov., an aerobic denitrifier from a leachate treatment plant

Catalytic properties of phenol carboxylase In vitro study of CO2: 4‐hydroxybenzoate isotope exchange reaction

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Catalytic properties of phenol carboxylase In vitro study of CO₂: 4‐hydroxybenzoate isotope exchange reaction