“…The rate-limiting step of lysis-cryptic growth is the lysis stage, and an increase of the lysis efficiency can therefore lead to an overall reduction of sludge production. Several methods have been applied for sludge disintegration so far: (i) thermal treatment in the temperature range from 40 C to 180 C [10,11], (ii) chemical treatment using acids or alkali [12], (iii) mechanical disintegration using ultrasounds, mills, and homogenizers [13][14][15][16][17][18][19][20], (iv) freezing and thawing [21], (v) biological hydrolysis with enzyme addition [22], (vi) advanced oxidation processes such as wet air oxidation, using H 2 O 2 and ozone [23][24][25][26], and (vii) combination ways such as thermo-chemical treatment [27][28][29], combination of alkaline and ultrasonic treatment [30]. But the comparison and evaluation of these processes for sludge lysis is not considered in this paper.…”