Anaerobic digestion of food waste – Effect of recirculation and temperature on performance and microbiology

Zamanzadeh, Mirzaman; Hagen, Live Heldal; Svensson, Kine; Linjordet, Roar; Horn, Svein Jarle

doi:10.1016/j.watres.2016.03.058

Cited by 238 publications

(85 citation statements)

References 44 publications

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“…In addition, functional insight was generated for two phylotypes affiliated with deeply branching phyla, Planctomycetes and the candidate phylum Atribacteria. Representatives from Planctomycetes are only sporadically observed in anaerobic digesters (33,34), while candidate phylum Atribacteria (formerly termed OP9) are widespread within these systems (7,15,35,36). Recent studies have demonstrated carbohydrate-degrading characteristics of uncultivated OP9 members (Atribacteria) (37,38), and the metabolic insight gained in this study also suggests proteolytic activity.…”

Section: Discussionmentioning

confidence: 59%

“…This illustrated that despite its low 16S rRNA abundance, M. thermophila is metabolically active. According to the wide range of NH 3 inhibition coefficients (K I,NH3 ) reported in the literature (14,15,48), the degree of process inhibition is highly dependent on the operational history of a digester and whether the microbial community within the digester has acclimatized to high ammonia exposure. Previous studies have shown that communities can adapt to stress and remain tolerant over several generation times (49).…”

Section: Discussionmentioning

confidence: 99%

“…This plant has been operating stably for a decade at 60°C, mainly using food waste as the substrate. The composition of this food waste has been published previously (15), but briefly it has a TCOD and ammonia content of around 270 g/liter and 500 mg/liter, respectively. Total solids (TS), volatile solids (VS), total chemical oxygen demand (TCOD), and soluble COD (SCOD) were analyzed according to standard methods (56).…”

Section: Methodsmentioning

confidence: 99%

“…Anaerobic oxidation of acetate and longer-chain fatty acids (FA) such as butyrate and propionate is thermodynamically unfavorable (ΔG Ͼ 0) under high partial pressure of H 2 (13) and requires a syntrophic interplay with hydrogen-consuming microorganisms such as hydrogenotrophic methanogens. Syntrophic networks become particularly significant in thermophilic anaerobic digestion, where a combination of high levels of free ammonia and high temperature inhibits acetoclastic methanogens (14)(15)(16).…”

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confidence: 99%

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Quantitative Metaproteomics Highlight the Metabolic Contributions of Uncultured Phylotypes in a Thermophilic Anaerobic Digester

Hagen

Frank

Zamanzadeh

et al. 2017

Appl Environ Microbiol

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In this study, we used multiple meta-omic approaches to characterize the microbial community and the active metabolic pathways of a stable industrial biogas reactor with food waste as the dominant feedstock, operating at thermophilic temperatures (60°C) and elevated levels of free ammonia (367 mg/liter NH 3 -N). The microbial community was strongly dominated (76% of all 16S rRNA amplicon sequences) by populations closely related to the proteolytic bacterium Coprothermobacter proteolyticus. Multiple Coprothermobacter-affiliated strains were detected, introducing an additional level of complexity seldom explored in biogas studies. Genome reconstructions provided metabolic insight into the microbes that performed biomass deconstruction and fermentation, including the deeply branching phyla Dictyoglomi and Planctomycetes and the candidate phylum "Atribacteria." These biomass degraders were complemented by a synergistic network of microorganisms that convert key fermentation intermediates (fatty acids) via syntrophic interactions with hydrogenotrophic methanogens to ultimately produce methane. Interpretation of the proteomics data also suggested activity of a Methanosaeta phylotype acclimatized to high ammonia levels. In particular, we report multiple novel phylotypes proposed as syntrophic acetate oxidizers, which also exert expression of enzymes needed for both the Wood-Ljungdahl pathway and ␤-oxidation of fatty acids to acetyl coenzyme A. Such an arrangement differs from known syntrophic oxidizing bacteria and presents an interesting hypothesis for future studies. Collectively, these findings provide increased insight into active metabolic roles of uncultured phylotypes and presents new synergistic relationships, both of which may contribute to the stability of the biogas reactor.IMPORTANCE Biogas production through anaerobic digestion of organic waste provides an attractive source of renewable energy and a sustainable waste management strategy. A comprehensive understanding of the microbial community that drives anaerobic digesters is essential to ensure stable and efficient energy production. Here, we characterize the intricate microbial networks and metabolic pathways in a thermophilic biogas reactor. We discuss the impact of frequently encountered microbial populations as well as the metabolism of newly discovered novel phylotypes that seem to play distinct roles within key microbial stages of anaerobic digestion in this stable high-temperature system. In particular, we draft a metabolic scenario whereby multiple uncultured syntrophic acetate-oxidizing bacteria are capable of syntrophically oxidizing acetate as well as longer-chain fatty acids (via the ␤-oxidation and Wood-Ljundahl pathways) to hydrogen and carbon dioxide, which methanogens subsequently convert to methane.

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Quantitative Metaproteomics Highlight the Metabolic Contributions of Uncultured Phylotypes in a Thermophilic Anaerobic Digester

Hagen

Frank

Zamanzadeh

et al. 2017

Appl Environ Microbiol

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“…has been related before to high ammonia levels (≥5 g NH 4 + -N L -1 ) in batch anaerobic reactors (Poirier et al, 2016). It seems that Caldicoprobacter spp., a sugar fermenter (Zamanzadeh et al, 2016), becomes substrate competitive to the other sugar fermenting bacteria, due to its increased tolerance to ammonia toxicity.…”

Section: Nitrososphaeramentioning

confidence: 99%

Effects of added enzymes on sorted, unsorted and sorted-out barley: A model study on realtime viscosity and process potentials using rapid visco analyser

Shetty

Zhuang

Olsen

et al. 2017

Journal of Cereal Science

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Many ammonia-rich biomass sources, such as manures and protein-rich substrates, are potential inhibitors of the anaerobic digestion (AD) process. It was previously demonstrated that bioaugmentation of Methanoculleus bourgensis MS2T in an ammonia inhibited process in a continuous stirred tank reactor (CSTR), resulted in up to 90 % recovery of the methane production compared to the uninhibited production. However, cultivation of pure strains has practical difficulties due to the need of special growth media and sterile conditions. In contrast, acclimatized enriched cultures have minor sterility requirements. In the current study, an enriched ammonia-tolerant methanogenic culture was bioaugmented in a CSTR reactor operating under ammonia-induced, inhibited-steady-state. The results demonstrated that bioaugmentation, completely counteracted the ammonia toxicity effect. This indicates that a commercial application of bioaugmentation could improve up to 36 % the methane production, the greenhouse gas reduction efficiency and the gross revenue of ammonia inhibited full scale biogas reactors. 16S rRNA gene sequencing showed that bioaugmentation changed the microbial composition of the reactors resulting in higher bacterial and lower archaeal community diversity. The bioaugmented reactor showed a fourfold increase of the abundance of the bioaugmented methanogens compared to the control reactor. This indicates that ammonia-tolerant methanogens established well in the ammonia-inhibited reactor and dominated over the domestic methanogenic population. Finally, this study showed that the enriched culture alleviated ammonia toxicity 25 % more efficiently than the previously used pure culture.

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Methane production and microbial community characteristics of anaerobic codigestion of straws and chicken manure

2022

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Previous studies showed that chicken manure and straws can be used as a feedstock in the production of methane during anaerobic digestions. The objective was to determine the best mixture of chicken manure and straw in the production of methane during anaerobic digestion. In this study the effect of three volatile solid (VS) rations on methane output, pH, hydrolase activity, and microbial community structure was determined. The optimal manure-straw VS ratio was M2 (9:1), where cumulative methane output reached a maximum of 227,660 ml kg -1 in 50 d. The cellulose activity was higher in the chicken manure to straw ratio of 9 than 4 or 1. The research showed that Megasphaera and Bacteroides were important cellulose-secreting bacteria. The results show: (a) during anaerobic digestion the cellulose and lignin contents were completely degraded; (b) protease, amylase, and lipase activities were higher in M2 (9:1) than M1 (1:0) or M3 (4:1); and (c) increasing amount of straw decreased bacteria and archaea diversity. Due to the addition of straw, the uniden-tified_Prevotellaceae had good physiological activity in the acidic environment between 5 and 30 d, Candidatus_Cloacimonas, unidentified_Lentimicrobiaceae, and Ruminiclostridium were the main archaea between 40 and 50 d.

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Anaerobic digestion of food waste – Effect of recirculation and temperature on performance and microbiology

Cited by 238 publications

References 44 publications

Quantitative Metaproteomics Highlight the Metabolic Contributions of Uncultured Phylotypes in a Thermophilic Anaerobic Digester

Quantitative Metaproteomics Highlight the Metabolic Contributions of Uncultured Phylotypes in a Thermophilic Anaerobic Digester

Effects of added enzymes on sorted, unsorted and sorted-out barley: A model study on realtime viscosity and process potentials using rapid visco analyser

Methane production and microbial community characteristics of anaerobic codigestion of straws and chicken manure

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