Anaerobic digestion of saline waste activated sludge and recovering raw sulfated polysaccharides

Liu, Jie; Zhang, Zi; Xue, Weiqi; Siriweera, Withanage B.; Chen, Guanghao; Wu, Di

doi:10.1016/j.biortech.2021.125255

Cited by 13 publications

(3 citation statements)

References 39 publications

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“…In contrast to some AOPs, which nonselectively degrade the polysaccharides, , the NH treatment here only transferred (not degraded) them from TB-EPS to LB-EPS and/or S-EPS. As the most abundant component of EPS, polysaccharide-based biomaterials have been reported to be recovered as high value-added products. − With further separation and purification, it showed great potential to recover high-value polysaccharide-based resources.…”

Section: Resultsmentioning

confidence: 99%

Simultaneously Enhanced Dewaterability and Biopolymer Release of Sludge by Natural Deep Eutectic Solvents: Performance, Mechanisms, and Insights of Theoretical Calculations

Zhai

Zhu

et al. 2022

ACS Sustainable Chem. Eng.

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The dewatering and resource recovery of wasteactivated sludge (WAS) seemed to be two contradictory processes due to the high moisture content induced by the extracellular polymer substances, which mainly consist of proteins and polysaccharides. Natural deep eutectic solvents (NADESs) were proposed to synergistically enhance dewaterability and biopolymer release of WAS. It showed that NADES with low-temperature pretreatment can significantly improve the dewaterability and release of valuable resources. With the addition of 1.0 mL of NADES combined with 60 °C thermal treatment for 120 min, the capillary suction time (CST) of sludge significantly decreased from 75.9 ± 7.5 to 30.5 ± 1.6 s, and 11.83 mg/L of the total amino acids (AAs) was obtained. The protein and polysaccharide contents were increased to 876.48 and 213.42 mg/L, respectively. This study indicated that NADES combined with low-temperature pretreatment is a green, cost-effective, and environmentally friendly method to improve sludge dewaterability and resource recovery simultaneously. The theoretical calculation results suggested that the generation of NADES•••AAs aggregates is an exothermic reaction and proceeds nonspontaneously. A good correlation is also found between the theoretically calculated free-energy change and the concentrations of AAs. On the basis of this study, the biopolymers from WAS can be recovered as value-added resources, enabling a recycling concept within the circular economy vision. The theoretical calculation can be used to guide future experiments for screening highly selective and effective NADES toward resource recovery from WAS.

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Section: Resultsmentioning

confidence: 99%

Simultaneously Enhanced Dewaterability and Biopolymer Release of Sludge by Natural Deep Eutectic Solvents: Performance, Mechanisms, and Insights of Theoretical Calculations

Zhai

Zhu

et al. 2022

ACS Sustainable Chem. Eng.

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“…Thus, knowing the exact chemical structure of the sulfated glycoconjugates can aid in finding sulfated polymer production pathways. At present, the methodologies used to study the sulfated glycoconjugates in the EPS depend on dye-spectrometric methods, i.e., visualization by Alcian blue staining and heparin red staining and quantification by DMMB staining. ,,, These methods are useful for indicating the presence of sulfated GAGs or other sulfated polymers but have difficulty in distinguishing between different types of sulfated polymers. Sensitive methods such as, e.g., MS/MS or liquid chromatography (LC)-fluorescence and LC-mass spectrometry (MS), could be used to distinguish between the types of sulfated polymers .…”

Section: Discussionmentioning

confidence: 99%

Sialylation and Sulfation of Anionic Glycoconjugates Are Common in the Extracellular Polymeric Substances of Both Aerobic and Anaerobic Granular Sludges

Chen,

de Bruin,

Pronk

et al. 2023

Environ. Sci. Technol.

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Anaerobic and aerobic granular sludge processes are widely applied in wastewater treatment. In these systems, microorganisms grow in dense aggregates due to the production of extracellular polymeric substances (EPS). This study investigates the sialylation and sulfation of anionic glyconconjugates in anaerobic and aerobic granular sludges collected from full-scale wastewater treatment processes. Size exclusion chromatography revealed a wide molecular weight distribution (3.5 to >5500 kDa) of the alkaline-extracted EPS. The high-molecular weight fraction (>5500 kDa), comprising 16.9–27.4% of EPS, was dominant with glycoconjugates. Mass spectrometry analysis and quantification assays identified nonulosonic acids (NulOs, e.g., bacterial sialic acids) and sulfated groups contributing to the negative charge in all EPS fractions. NulOs were predominantly present in the high-molecular weight fraction (47.2–84.3% of all detected NulOs), while sulfated glycoconjugates were distributed across the molecular weight fractions. Microorganisms, closely related to genera found in the granular sludge communities, contained genes responsible for NulO and sulfate group synthesis or transfer. The similar distribution patterns of sialylation and sulfation of the anionic glycoconjugates in the EPS samples indicate that these two glycoconjugate modifications commonly occur in the EPS of aerobic and anaerobic granular sludges.

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“…Then the mixture was immersed in an ice water bath for cooling for 15 min. the mixture was measured the absorbance value at a wavelength of 625 nm, and calculated the polysaccharide content of the sample solution based on the standard curve of glucose content [41,42].…”

Section: Analysis Proceduresmentioning

confidence: 99%

Micro-Nano Bubbles Conditioning Treatment of Contaminated Sediment for Efficient Reduction: Dehydration Characteristic and Mechanism

Zhang

Zhao²,

Yin³

et al. 2023

Water

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The reduction and dehydration treatment of contaminated sediment from rivers and lakes is a prerequisite for ensuring the subsequent safe disposal. In this study, micro-nano bubbles (MBs) technology was creatively proposed for the conditioning treatment of contaminated sediment to improve its sedimentation and dehydration performance. Orthogonal experiment and single factor experiment were conducted to optimize factors such as bubble size, intake air volume and treatment time. The conditioning effect was analyzed through direct and indirect characterization parameters. The results showed that the range (R) values for bubble size, intake air volume and treatment time were 101.8, 94.5 and 51.6 respectively in the orthogonal analysis. The optimum bubble size, intake air volume and treatment time were 1 μm, 30 L/min and 90 s. At this time, the CST of conditioned sediment decreased to 160.6 s (the reduction rate of 89.29%) and the moisture content of the filter cake decreased to 65.2%. Through the analysis of polysaccharide and MLSS, it was found that the MBs effectively exerted the oxidation and extracellular polymer cracking properties, which released polysaccharides that easily bind to water. The SEM analysis of the filter cake showed a loose structure and rich porosity compared to the undisturbed sediment. Meanwhile, the MBs promoted the homogenization degree of conditioners to achieve efficient dehydration. Therefore, MBs conditioning was verified as a novel and promising technique for improving the dehydration performance of river and lake contaminated sediment.

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Anaerobic digestion of saline waste activated sludge and recovering raw sulfated polysaccharides

Cited by 13 publications

References 39 publications

Simultaneously Enhanced Dewaterability and Biopolymer Release of Sludge by Natural Deep Eutectic Solvents: Performance, Mechanisms, and Insights of Theoretical Calculations

Simultaneously Enhanced Dewaterability and Biopolymer Release of Sludge by Natural Deep Eutectic Solvents: Performance, Mechanisms, and Insights of Theoretical Calculations

Sialylation and Sulfation of Anionic Glycoconjugates Are Common in the Extracellular Polymeric Substances of Both Aerobic and Anaerobic Granular Sludges

Micro-Nano Bubbles Conditioning Treatment of Contaminated Sediment for Efficient Reduction: Dehydration Characteristic and Mechanism

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