Anaerobic Infections

Sl, Gorbach; Jg, Bartlett

doi:10.1056/nejm197406062902305

Cited by 134 publications

(47 citation statements)

References 17 publications

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“…B. gingivalis has been repeatedly isolated in periodontitis (Slots 1979) and was the dominant organism, comprising 32% of the cultivable flora in one study of advanced periodontitis. B. gingivalis was an essential component of experimental mixed anaerobic infections (MacDonald 1962), readily produced absesses in animal models (Takazoe et al 1971), and was a predominant member of anaerobic pleuropulmonary infections (Bartlett & Finegold 1972) and anaerobic infections of soft tissues (Gorbach & Bartlett 1974). Both subspecies of B. melaninogenicus are frequent isolates from subgingival sites exhibiting gingivitis (Socransky 1977).…”

Section: Discussionmentioning

confidence: 99%

Collagenolytic activity associated with Bacteroides species and Actinobacillus actinomycetemcomitans

Robertson

Lantz

Marucha

et al. 1982

J of Periodontal Research

178

120

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Collagenolytic activity was assessed in a variety of microorganisms with particular emphasis on members of the indigenous oral flora. Organisms were grown in complete and peptide depleted basal anaerobic broth. Cell sonicates and media preparations were assayed for collagenolytic activity using 14C‐labelled collagen in solution and as fibrils. Assay reaction products were evaluated by acrylamide gel electrophoresis. All tested species of Bacteroides, including B. gingivalis, B, melaninogenicus ss. melaninogenicus and intermedius, B. capillus, B. oris, B. thetaiotaomicron, and B. fragilis produced collagenase which was primarily associated with the cell fraction. Collagenolytic activity was also observed in both media and cell sonicates of Actinobacillus actinomycetemcomitans, Strain 511. The Bacteroides and Actinobacillus enzymes were heat labile, inhibited by EDTA and human serum. Enzyme activity appeared to be enhanced when these organisms were grown in peptide depleted medium. Collagenase production by tested species of Bacteroides and A. actinomycetemcomitans (511) was unique among other members of the oral microflora including species of Fusobacterium, Actinomyces, Capnocylophaga, and Selenomonas, which did not demonstrate collagenolytic activity under the same cultural conditions.

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Section: Discussionmentioning

confidence: 99%

Collagenolytic activity associated with Bacteroides species and Actinobacillus actinomycetemcomitans

Robertson

Lantz

Marucha

et al. 1982

J of Periodontal Research

178

120

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“…Bacteroides fragilis, a Gram-negative obligatory anaerobic bacillus, is the most frequently isolated anaerobic species from intraperitoneal and intra-abdominal infections in humans (Gorbach and Bartlett, 1974;Salyers, 1984;Finegold, 1989). B. fragilis is an opportunistic pathogen and reaches its sites of infection as a result of trauma, surgery or in¯ammation and the concomitant spillage of colonic contents.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the BatI (Bacteroides aerotolerance) operon in Bacteroides fragilis: isolation of a B. fragilis mutant with reduced aerotolerance and impaired growth in in vivo model systems

Tang

Dallas

Malamy

1999

Molecular Microbiology

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YT135.2.8, a Tn4400′ insertion mutant of Bacteroides fragilis strain TM4000, grows poorly when used to infect Monika or Chinese hamster ovary (CHO) cell monolayers and is outcompeted by wild‐type strains in mixed infections. YT135.2.8 also shows defects in the rat granuloma pouch model system in monoculture and is completely outcompeted by the wild‐type strain in a mixed infection. In addition, this mutant shows defects in a new model system consisting of CHO suspension cell columns. All of these defects may be explained by the finding that YT135.2.8 shows decreased tolerance to exposure to atmospheric oxygen (less aerotolerant). The monolayer growth defect (MGD) of YT135.2.8 can be influenced significantly by the presence of sulphur‐containing reducing agents (cysteine, dithiothreitol, thiodiglycol) or the non‐sulphur reducing agent Tris‐(2‐carboxylethyl)phosphine (TCEP). The defects in YT135.2.8 can be complemented by a 6.6 kb fragment of the B. fragilis chromosome. DNA sequencing of this fragment and of the regions flanking the Tn4400′ insertion in the B. fragilis chromosome revealed the presence of five open reading frames, corresponding to genes bat (Bacteroides aerotolerance) A, B, C, D, E, which form the BatI operon; Tn4400′ inserted within batD. All of the hypothetical proteins possess one or more membrane‐spanning domains. BatA and BatB show high similarity to each other but, like BatD, they show no match to sequences of known function in the databases. BatC and BatE contain 2–4 repeated sequences similar to the tetratricopeptide repeats (TPRs) seen in many eukaryotic proteins. The function of TPR sequences in protein interactions in other systems leads to the suggestion that the Bat proteins form a complex. The BatI complex may be involved in the generation or export of reducing power equivalents to the periplasm of the B. fragilis cell.

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“…In 1974, Gorbach and Bartlett [39–41] reviewed the available literature on pyogenic processes associated with anaerobic organisms of the normal flora and provided your expertise. These authors stated that anaerobic bacteria are rarely encountered in pyogenic arthritis.…”

Section: Discussionmentioning

confidence: 99%

Pyogenic arthritis of native joints due to Bacteroides fragilis

et al. 2016

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Pyogenic arthritis of native joints due to Bacteroides fragilis seems to be an infrequent disease. We analyzed the cases diagnosed in a tertiary hospital during a 22-year period and reviewed the literature to summarize the experience with this infectious entity.In our institution, of 308 patients with pyogenic arthritis of native joints, B fragilis was the causative organism in 2 (0.6%) cases. A MEDLINE search (1981–2015) identified 19 additional cases.Of the 21 patients available for review (13 men and 8 women, with a mean age, of 54.4 ± 17 years), 19 (90%) presented a systemic predisposing factor for infection; the most common associated illness was rheumatoid arthritis (8 patients). Bacteremia was documented in 65% (13/20) of cases. In 5 patients (24%), 1 or more concomitant infectious process was found. Metronidazole was the most frequently used antibiotic. Surgical drainage was performed in 11 cases (52%). The overall mortality rate was 5%.Pyogenic arthritis of native joints due to B fragilis is an infrequent disease that mainly affects elderly patients with underlying medical illnesses and in whom bacteremia and the presence of a concomitant infectious process are frequent conditions.

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Anaerobic Infections

Cited by 134 publications

References 17 publications

Collagenolytic activity associated with Bacteroides species and Actinobacillus actinomycetemcomitans

Collagenolytic activity associated with Bacteroides species and Actinobacillus actinomycetemcomitans

Characterization of the BatI (Bacteroides aerotolerance) operon in Bacteroides fragilis: isolation of a B. fragilis mutant with reduced aerotolerance and impaired growth in in vivo model systems

Pyogenic arthritis of native joints due to Bacteroides fragilis

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