Anaerobic Mineralization of Toluene by Enriched Sediments with Quinones and Humus as Terminal Electron Acceptors

Cervantes, Francisco J.; Dijksma, Wouter; Duong-Dac, Tuan; Иванова, А. Е.; Lettinga, G.; Field, Jim A.

doi:10.1128/aem.67.10.4471-4478.2001

Cited by 96 publications

(54 citation statements)

References 42 publications

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“…When the growth of the partner methanogen was inhibited by 2-BES, AQDS was able to replace methanogen as an electron acceptor and only acetate (and CO 2 ) was produced. This has been predicted by Cervantes et al (5,6), using methanogenic phenol-degrading enrichment cultures. These findings strongly indicated that AQDS could serve as an alternate electron acceptor in the group TA members.…”

Section: Fig 4 Phylogenetic Position Of Strain Uisupporting

confidence: 67%

Syntrophorhabdus aromaticivorans gen. nov., sp. nov., the First Cultured Anaerobe Capable of Degrading Phenol to Acetate in Obligate Syntrophic Associations with a Hydrogenotrophic Methanogen

Qiu

Hanada

Ohashi

et al. 2008

Appl Environ Microbiol

229

136

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Phenol degradation under methanogenic conditions has long been studied, but the anaerobes responsible for the degradation reaction are still largely unknown. An anaerobe, designated strain UI T , was isolated in a pure syntrophic culture. This isolate is the first tangible, obligately anaerobic, syntrophic substrate-degrading organism capable of oxidizing phenol in association with an H 2 -scavenging methanogen partner. Besides phenol, it could metabolize p-cresol, 4-hydroxybenzoate, isophthalate, and benzoate. During the degradation of phenol, a small amount of 4-hydroxybenzoate (a maximum of 4 M) and benzoate (a maximum of 11 M) were formed as transient intermediates. When 4-hydroxybenzoate was used as the substrate, phenol (maximum, 20 M) and benzoate (maximum, 92 M) were detected as intermediates, which were then further degraded to acetate and methane by the coculture. No substrates were found to support the fermentative growth of strain UI T in pure culture, although 88 different substrates were tested for growth. 16S rRNA gene sequence analysis indicated that strain UI T belongs to an uncultured clone cluster (group TA) at the family (or order) level in the class Deltaproteobacteria. Syntrophorhabdus aromaticivorans gen. nov., sp. nov., is proposed for strain UI T , and the novel family Syntrophorhabdaceae fam. nov. is described. Peripheral 16S rRNA gene sequences in the databases indicated that the proposed new family Syntrophorhabdaceae is largely represented by abundant bacteria within anaerobic ecosystems mainly decomposing aromatic compounds.Phenols and phthalate isomers are among the most widely used chemicals and are often found in industrial wastewaters in abundance. These chemicals are known to be inhibitors for the growth of microorganisms in biological treatment processes and are regarded as priority pollutants on the U.S. Environmental Protection Agency list (19,38). Phenol has long been known to be degraded anaerobically in the methanogenic environment (20, 43). Methanogenic degradation of phenol is significant considering the wastewater treatment processes of industrial waste chemicals and biogeochemistry of naturally occurring phenolic compounds in deep subsurface environments, such as an oil reservoir. However, despite the tremendous effort to search for phenol degraders in such environments, only a few examples are known. To date, only one species, Cryptanaerobacter phenolicus, has been isolated and characterized as an anaerobe able to metabolize phenol under methanogenic conditions (16). The organism is an anaerobic bacterium that can transform phenol into benzoate in the presence of as-yet-unidentified electron donors. However, no organisms that are genuinely capable of utilizing phenol as the sole energy source under methanogenic conditions have been isolated so far.Recently, the enrichment and identification of mesophilic phthalate isomer-degrading bacteria from methanogenic sludges treating wastewater from the manufacture of terephthalic and isophthalic acids were reported (29). Thro...

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Section: Fig 4 Phylogenetic Position Of Strain Uisupporting

confidence: 67%

Syntrophorhabdus aromaticivorans gen. nov., sp. nov., the First Cultured Anaerobe Capable of Degrading Phenol to Acetate in Obligate Syntrophic Associations with a Hydrogenotrophic Methanogen

Qiu

Hanada

Ohashi

et al. 2008

Appl Environ Microbiol

229

136

View full text Add to dashboard Cite

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“…These reactions may take place under Fe(III)-reducing, denitrifying, and sulfate-reducing conditions, by anoxygenic photosynthetic bacteria, or in syntrophic consortia of proton-reducing and methanogenic bacteria. Other terminal electron acceptors shown to be used during anaerobic hydrocarbon metabolism include manganese oxides (357,358), soil humic acids and the humic acid model compound anthraquinone-2,6-disulfonate (105), and fumarate in a fermentative oxidation process (420). Mixed-culture work continues as enhanced bioremediation strategies are tested (17,530) and new metabolites are described (23,172,421,687).…”

Section: Anaerobic Hydrocarbon Metabolismmentioning

confidence: 99%

Recent Advances in Petroleum Microbiology

Hamme¹,

Singh

Ward

2003

Microbiol Mol Biol Rev

1,201

640

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Recent advances in molecular biology have extended our understanding of the metabolic processes related to microbial transformation of petroleum hydrocarbons. The physiological responses of microorganisms to the presence of hydrocarbons, including cell surface alterations and adaptive mechanisms for uptake and efflux of these substrates, have been characterized. New molecular techniques have enhanced our ability to investigate the dynamics of microbial communities in petroleum-impacted ecosystems. By establishing conditions which maximize rates and extents of microbial growth, hydrocarbon access, and transformation, highly accelerated and bioreactor-based petroleum waste degradation processes have been implemented. Biofilters capable of removing and biodegrading volatile petroleum contaminants in air streams with short substrate-microbe contact times (<60 s) are being used effectively. Microbes are being injected into partially spent petroleum reservoirs to enhance oil recovery. However, these microbial processes have not exhibited consistent and effective performance, primarily because of our inability to control conditions in the subsurface environment. Microbes may be exploited to break stable oilfield emulsions to produce pipeline quality oil. There is interest in replacing physical oil desulfurization processes with biodesulfurization methods through promotion of selective sulfur removal without degradation of associated carbon moieties. However, since microbes require an environment containing some water, a two-phase oil-water system must be established to optimize contact between the microbes and the hydrocarbon, and such an emulsion is not easily created with viscous crude oil. This challenge may be circumvented by application of the technology to more refined gasoline and diesel substrates, where aqueous-hydrocarbon emulsions are more easily generated. Molecular approaches are being used to broaden the substrate specificity and increase the rates and extents of desulfurization. Bacterial processes are being commercialized for removal of H2S and sulfoxides from petrochemical waste streams. Microbes also have potential for use in removal of nitrogen from crude oil leading to reduced nitric oxide emissions provided that technical problems similar to those experienced in biodesulfurization can be solved. Enzymes are being exploited to produce added-value products from petroleum substrates, and bacterial biosensors are being used to analyze petroleum-contaminated environments

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“…On the other hand, microbial-mediated humus reduction may highly be related to the anaerobic biodegradation of organic or inorganic pollutants (Gu et al 2005;Wang et al 2009). Previous literatures have reported that AQDS (and humic substances) can be utilized by microbes as effective electron acceptors or shuttles for the oxidative degradation of organic pollutants, such as 1,2-dichloroethylene, vinyl chloride, toluene, and phenolic compounds (Bradley et al 1998;Cervantes and Dijksma 2001;Cervantes et al 2000). Therefore, the newly isolated humus-reducing strain of P. aeruginosa holds great potentials for microbial remediation of organic pollutants, especially in the contaminated soil enriched with HS.…”

Section: Effect Of Electron Donors On Aqds Reduction By Strain Pah-1mentioning

confidence: 99%

“…Among the above reported electron acceptors, HS that are more abundant and active components in the natural environments have great potentials to serve as terminal electron acceptors in microbial respiration (Van Trump et al 2006;Perminova et al 2005;Straub et al 2005). Furthermore, due to its important role in the anaerobic biodegradation of organic pollutants, the microbial-mediated HS reduction has been attracted significant research concerns (Bradley et al 1998;Cervantes et al 2000;Cervantes and Dijksma 2001). Though HS are highly expected to play an important role in the PAHs natural degradation, there has no research studying PAHs anaerobic degradation coupling with HS reduction by pure culture.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic degradation of phenanthrene by a newly isolated humus-reducing bacterium, Pseudomonas aeruginosa strain PAH-1

Wang

et al. 2011

J Soils Sediments

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Purpose The anaerobic degradation of polycyclic aromatic hydrocarbons (PAHs) has great significance to PAHs' natural attenuation in contaminated sites. Previous studies mainly focused on anaerobic PAH degradation by mixed cultures with nitrate, sulfate, or Fe(III) oxides as electron acceptors, and the roles of pure cultures in the process was rarely reported. The aim of this paper is to isolate a pure culture that is capable of degrading phenanthrene anaerobically with anthraquinone-2,6-disulphonate (AQDS) as the sole electron acceptor and evaluate its environmental functions. Materials and methods A strain of pure culture was isolated from anodic solution of a microbial fuel cell via enrichment procedure with phenanthrene and AQDS under anaerobic conditions. Using the single colony isolation technique, a distinct colony was obtained and identified by the phenotypic and phylogenetic analysis. Its ability to reduce AQDS and degrade phenanthrene was conducted in serum bottles by standard anaerobic techniques (purged with 80% N 2 −20% CO 2 ). The concentration of AH 2 QDS and fructose was quantified by UV-vis spectrophotometer at 450 and 210 nm, respectively. Cells number was determined by direct plate counting on aerobic LB agar medium. The concentration of phenanthrene was determined using HPLC. Results and discussion Strain PAH-1 was identified as Pseudomonas aeruginosa. It could oxidize fructose or glucose to reduce AQDS and can support microbial growth by conserving energy from fructose to AQDS. It has the ability to degrade phenanthrene directly with AQDS as the sole electron acceptor (46.5% removal), and the microbial process may be AQDS dependent. The addition of small organic substances (fructose) could enhance the anaerobic biodegradation of phenanthrene from 46.5% to 56.7%. The anaerobic degradation of phenanthrene fits the pseudo-firstorder kinetics, giving the rate constants of 0.0233/day (R 2 =0.934) and 0.0328/day (R 2 =0.933) for non-fructose set and fructose set, respectively. Conclusions We successfully isolated a facultative anaerobe, P. aeruginosa strain PAH-1. This study was the first paper reporting that a pure culture (strain PAH-1) has the ability to anaerobically degrade phenanthrene with AQDS as the sole electron acceptor. The finding also explores the environmental significance of the Pseudomonas genus.

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Anaerobic Mineralization of Toluene by Enriched Sediments with Quinones and Humus as Terminal Electron Acceptors

Cited by 96 publications

References 42 publications

Syntrophorhabdus aromaticivorans gen. nov., sp. nov., the First Cultured Anaerobe Capable of Degrading Phenol to Acetate in Obligate Syntrophic Associations with a Hydrogenotrophic Methanogen

Syntrophorhabdus aromaticivorans gen. nov., sp. nov., the First Cultured Anaerobe Capable of Degrading Phenol to Acetate in Obligate Syntrophic Associations with a Hydrogenotrophic Methanogen

Recent Advances in Petroleum Microbiology

Anaerobic degradation of phenanthrene by a newly isolated humus-reducing bacterium, Pseudomonas aeruginosa strain PAH-1

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