Anaerococcus jeddahensis sp. nov., a New Bacterial Species Isolated From Healthy Nomadic Bedouin Woman From Saudi Arabia

Abstract

An understanding of the microbial diversity of the human body has generated significant interest in recent years. With the advent of MALDI-TOF mass spectrometry, high-speed sequencing, and the rebirth of microbial culture, knowledge of human microbiota is growing. Using culturomics, a strategy to explore the microbial diversity of samples, coupled with a taxono-genomic strategy, we isolated a new bacterium named Anaerococcus jeddahensis sp. nov. strain SB3. This strain was isolated from the stool sample of a healthy nomadic Bedouin woman from Saudi Arabia. Here, we describe the characteristics of this organism, and the complete genome sequence and annotation. Strain SB3 is a Gram-positive obligate anaerobic coccus which is non-motile and non-spore forming. Fatty acid analysis shows that the major fatty acid is by far hexadecanoic acid (C16:0; 52%). Its genome is 1,903,534 bp long and has 29.70 mol% of G+C content. It contains 1756 protein-coding genes and 53 RNA genes. These results show that strategy provides a better understanding of the microorganism and that is a good methodology for microbial identification and characterization.

Anaerococcus ihuae sp. nov. and Mediannikoviicoccus vaginalis gen. nov., sp. nov., two new bacteria isolated from human vaginal samples

“… Strains: 1 , Anaerococcus ihuae Marseille-Q5893; 2 , Anaerococcus vaginalis PH9 (Hugon et al 2012 ); 3 , Anaerococcus rubiinfantis mt16 (Alou et al 2016 ); 4 , Anaerococcus jeddahensis SB3 (Dione et al 2018 ) ; 5 , Mediannikoviicoccus vaginalis Marseille-Q5883; 6 , Peptoniphilus obesi ph1 (Mishra et al 2013 ); 7 , Peptoniphilus duerdenii WAL 18,896 (Ulger-Toprak et al 2012 ); 8 , Parvimonas micra ATCC 33270 (Murdoch and Shah 1999 ); 9 , Finegoldia magna ATCC 15794 (Murdoch and Shah 1999 ); + , positive; − negative; w, weakly positive; d, different reactions …”

Human milk microbial species are associated with infant head-circumference during early and late lactation in Guatemalan mother-infant dyads

“…However, some have described Staphylococcus_epidermidis as an opportunistic species ( Coates et al, 2014 ), given that it has been isolated from maternal milk of mothers diagnosed with mastitis ( Delgado et al, 2009 ). Similarly, Anaerococcus_2 , is an uncharacterized species with several members found in the human vagina, on skin, and in nasal cavities, but it can be involved in human infections ( Song et al, 2007 ; Pépin et al, 2011 ; Dione et al, 2018 ). However, human milk is suggested to have the non-virulent strains of these species in early lactation ( Soeorg et al, 2017 ).…”

Peptoniphilus Colimassiliensis sp. nov. and Peptoniphilus Urinimassiliensis sp. nov., Two New Species Isolated From Humans

“…The morphology of the bacterial cells of each strain was highlighted using a TM4000 microscope (Hitachi Group, Krefeld, Germany). For each strain, 50 mg of bacterial biomass was collected from several culture plates in order to prepare the fatty acid methyl ester analysis, as previously described (Dione et al 2018).…”