Anaerococcus urinimassiliensis sp. nov., a new bacterium isolated from human urine

Abstract

To date there are thirteen species validly assigned to the genus Anaerococcus. Most of the species in this genus are anaerobic and of human origin. Anaerococcus urinimassiliensis sp. nov., strain Marseille-P2143T is member of family Peptoniphilaceae, which was isolated from the urine of a 17-year-old boy affected by autoimmune hepatitis and membranoproliferative glomerulonephritis using the culturomic approach. In the current study, a taxono-genomics method was employed to describe this new species. The strain Marseille-P2143T was gram positive cocci with translucent colonies on blood agar. Its genome was 2,189,509 bp long with a 33.5 mol% G + C content and exhibited 98.48% 16S rRNA similarity with Anaerococcus provencensis strain 9,402,080. When Anaerococcus urinomassiliensis strain Marseill-P2143T is compared with closely related species, the values ranged from 71.23% with A. hydrogenalis strain DSM 7454T (NZ_ABXA01000052.1) to 90.64% with A. provencensis strain 9402080T (NZ_HG003688.1). This strain has implemented the repertoire of known bacteria of the human urinary tract.

Adapted Protocol for Saccharibacteria Cocultivation: Two New Members Join the Club of Candidate Phyla Radiation

“…tRNA genes were predicted by tRNA SCAN SE, using the default option and all available sequence sources ( 40 ). Proteomes were predicted with BLASTp (E value of 0.001, minimum coverage and identity of 70% and 30%, respectively) against the Cluster of Orthologous Groups database ( 41 ). Antibiotic resistance genes were then predicted using the adapted strategy for CPR that was recently described in Maatouk et al ( 15 ).…”

Human nasal microbiota shifts in healthy and chronic respiratory disease conditions

“…Despite the differences in the healthy group, several bacteria, which could be considered as candidates for beneficial microorganisms, were more commonly identified in healthy and less common in BE patients' nasal microbiomes, for instance, Corynebacterium accolens, Anaerococcus octavius, Anaerococcus urinomassiliensis, Peptoniphilus lacydonensis, Peptoniphilus grossensis and Snodgrassella alvi. Some of these bacteria are already known players of healthy microbiota and their protective properties have been previously described [47][48][49]. However, additional research into each of them should be carried out, since some of them also have been observed to exhibit pathogenic properties in certain situations [50,51].…”