Analgesic activity of Ugni molinae (murtilla) in mice models of acute pain

Aim: Acetazolamide (AZA), a carbonic anhydrase (CA) inhibitor, has been found to alleviate inflammatory and neuropathic pain in rats. In the present study, we investigated the effects of AZA on thermal-and chemical-stimulated acute pain in mice and the possible mechanisms underlying the effects. Methods: Five acute pain models based on thermal and chemical stimuli were established to investigate the effects of AZA on different types of nociception in mice. The antinociceptive effects of methazolamide (another CA inhibitor) and diazepam (a positive allosteric modulator of GABA A receptor) were also examined. The drugs were administered either intraperitoneally (ip) or intrathecally. Results: AZA (50-200 mg/kg, ip) did not produce analgesia in two thermal-stimulated acute pain models, ie, mouse tail-flick and hotplate tests. In contrast, AZA (50-200 mg/kg, ip) dose-dependently reduced paw licking time in both capsaicin and formalin tests in mice. A similar result was observed in a mouse acetic acid-induced writhing test. However, AZA (10 nmol/mouse, intrathecally) did not produce significant analgesia in the 3 chemical-stimulated acute pain models. In addition, methazolamide (50-200 mg/kg, ip) and diazepam (0.25-1.0 mg/kg, ip) did not produce significant analgesia in either thermal-or chemical-stimulated acute pain. Conclusion: AZA produces analgesia in chemical-stimulated, but not thermal-stimulated acute pain in mice. The attenuation of chemical-stimulated acute pain by AZA may not be due to enhancement of GABA A receptor-mediated inhibition via inhibiting CA activity but rather a peripheral ion channel-related mechanism.

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“…The method is an adaptation of that described by Delporte et al [12] . Lower and higher light intensities of 8 and 12, respectively, were used.…”

Section: Tail-flick Testmentioning

confidence: 99%

Acetazolamide attenuates chemical-stimulated but not thermal-stimulated acute pain in mice

Sun

Chen²,

Peng³

et al. 2013

Acta Pharmacol Sin

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“…In agreement, many species of Tephrosia genus present flavonoids (Arriaga et al, 2009a;Arroio et al, 2004;Jang et al, 2003). There is evidence that isolated flavonoids reduce mechanical and thermal hyperalgesia (e.g., increased response to nociceptive stimuli; pain) by activating the endogenous opioid system (Anjaneyulu & Chopra, 2003;Delporte et al, 2007;Loscalzo et al, 2008Loscalzo et al, , 2011 and inhibition of hyperalgesic cytokines production (Chou et al, 2003;Kandhare et al, 2012;Valério et al, 2009b;Valsecchi et al, 2008).…”

Section: Introductionmentioning

confidence: 71%

“…Flavonoids are phenolic compounds with analgesic activity (Anjaneyulu & Chopra, 2003;Chou et al, 2003;Delporte et al, 2007;Kandhare et al, 2012;Loscalzo et al, 2008Loscalzo et al, , 2011Valério et al, 2009b;Valsecchi et al, 2008). Preliminary results detected the presence of flavonoids including novel compounds in the Tephrosia sinapou ethyl acetate extract and others have also shown the presence of flavonoids in Tephrosia genus plants (Arriaga et al, 2009a;Arroio et al, 2004;Jang et al, 2003).…”

Section: Discussionmentioning

confidence: 89%

Tephrosia sinapouethyl acetate extract inhibits inflammatory pain in mice: Opioid receptor dependent inhibition of TNFαand IL-1βproduction

Martínez¹,

Zarpelon²,

Cardoso³

et al. 2013

Pharmaceutical Biology

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Context: Tephrosia toxicaria is currently known as Tephrosia sinapou (Buc'hoz) A. Chev. (Fabaceae) and is a source of compounds such as flavonoids that inhibit inflammatory pain. Objective: To investigate the analgesic effect and mechanisms of the ethyl acetate extract of T. sinapou in inflammatory pain in mice. Materials and methods: Behavioral responses were evaluated using mechanical (1-24 h) and thermal hyperalgesia (0.5-5 h), writhing response (20 min) and rota-rod (1-5 h) tests. Neutrophil recruitment (myeloperoxidase activity), cytokines (tumor necrosis factor [TNF]a and interleukin [IL]-1b), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) serum levels were determined by colorimetric assays. Pharmacological treatments were opioid receptor antagonist (naloxone, 0.1-1 mg/kg) and control opioid (morphine, 5 mg/kg). Inflammatory stimuli were carrageenin (100 mg/paw), complete Freund's adjuvant (CFA, 10 ml/paw), prostaglandin E 2 (PGE 2 , 100 ng/paw) and acetic acid (0.8%). Results: The intraperitoneal pre-treatment with extract inhibited in a dose-dependent (30-300 mg/kg) dependent manner the mechanical hyperalgesia induced by carrageenin (up to 93% inhibition). The post-treatment (100 mg/kg) inhibited CFA-induced hyperalgesia (up to 63% inhibition). Naloxone (1 mg/kg) prevented the inhibitory effect of the extract over carrageenin-induced mechanical (100%) and thermal (100%) hyperalgesia, neutrophil recruitment (52%) and TNFa (63%) and IL-1b (98%) production, thermal threshold in naïve mice (99%), PGE 2 -induced mechanical hyperalgesia (88%) and acetic acid-induced writhing response (49%). There was no significant alteration in the rota-rod test, and AST and ALT serum levels by extract treatment. Discussion and conclusion: Tephrosia sinapou ethyl acetate extract reduces inflammatory pain by activating an opioid receptor-dependent mechanism.

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“…Por otra parte, la rizogénesis in vitro y el cultivo de células a partir de callos podría ser utilizada para la producción de metabolitos secundarios en bioreactores, lo cual resulta interesante para una planta a la cual se le atribuyen numerosas característi-cas medicinales, como efectos analgésicos, anti-inflamatorios, astringentes, actividad antimicrobiana y antioxidante de sus hojas (Delporte et al 2007). Por otra parte, los callos pueden ser utilizados para hacer suspensiones celulares para la producción de embriones somáticos.…”

Section: Introductionunclassified

Inducción in vitro de callogénesis y organogénesis indirecta a partir de explantes de cotiledón, hipocótilo y hoja en Ugni molinae

et al. 2014

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Analgesic activity of Ugni molinae (murtilla) in mice models of acute pain

Cited by 47 publications

References 16 publications

Acetazolamide attenuates chemical-stimulated but not thermal-stimulated acute pain in mice

Acetazolamide attenuates chemical-stimulated but not thermal-stimulated acute pain in mice

Tephrosia sinapouethyl acetate extract inhibits inflammatory pain in mice: Opioid receptor dependent inhibition of TNFαand IL-1βproduction

Inducción in vitro de callogénesis y organogénesis indirecta a partir de explantes de cotiledón, hipocótilo y hoja en Ugni molinae

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