Analgesic and Anti-Inflammatory Activities of 80% Methanol Extract and Solvent Fractions of Ehretia cymosa Thonn (Boraginaceae) Leaves in Rodents

Abstract

Background Ethnobotanical studies in various districts of Ethiopia reported that Ehretia cymosa ( E. cymosa ) is used for the management of headache, abdominal pain, arthritis and rheumatism. However, there is no scientific investigation done so far to confirm these traditional claims. Thus, the aim of this study was to assess the analgesic and anti-inflammatory effects of the 80% methanol extract and fractions of E. cymosa leaves. Methods The dried and pulverized leaves of E. cymosa were soaked with 80% methanol to obtain a crude extract. Fractionation was done using chloroform, ethyl acetate and water by a soxhlet apparatus. The analgesic effects of the crude extract and solvent fractions were assessed using acetic acid-induced writhing and hot plate tests whereas anti-inflammatory activities were investigated using carrageenan-induced paw edema and cotton-pellet-induced granuloma models. Results In all the tested doses, the 80% methanol extract and solvent fractions revealed substantial (p < 0.001) analgesic activities in acetic acid induced writhing test. In the hot plate method, all the tested doses of E. cymosa crude extract and the solvent fractions produced significant analgesic activities (p < 0.05). In the carrageenan-induced acute inflammation model, all tested doses of the crude extract and solvent fractions resulted in a significant decline in paw edema. The 80% methanol extract and solvent fractions of E. cymosa at all the tested doses significantly reduced inflammatory exudates and granuloma mass formations (p < 0.001). Conclusion From the results of this investigation, it can be stated that 80% methanol extract, aqueous, ethyl acetate and chloroform fractions of E. cymosa exhibited considerable analgesic and anti-inflammatory activities, supporting the plant’s traditional use as a remedy for a variety of painful and inflammatory conditions.

Phytochemical and Trace elements Variation in different traditional medicinal plant species of Peren District, Nagaland

“…solvent mediums(Patel et al 2011;Truong et al 2019,). These observations propose that organic solvent extraction can serve as a suitable approach to validate the therapeutic properties of medicinal plants, also supported by other studies(Galvez et al 2005;Kumar et al 1997;Ashagrie et al 2023). The choice of solvent signi cantly affects predicting bioactive compounds from plant material.…”

Investigation of the Inflammatory, Antipyretic, and Analgesic Potential of Ethanol Extract from Hedyotis capitellata Wall. ex G. Don Leaves in Mice

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