Analgesic and anti-inflammatory activity of a crude root extract of Pfaffia glomerata (Spreng) Pedersen

Neto, Amato; Costa, Jean; Belati, C. C.; Vinholis, Adriana Helena Chicharo; Possebom, L. S.; Filho, Ademar A. Da Silva; Cunha, Wilson Roberto; Carvalho, J.C.T.; Bastos, Jairo Kenupp; Silva, M. L. A.

doi:10.1016/j.jep.2004.08.035

Cited by 101 publications

(57 citation statements)

References 21 publications

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“…Crude extract were obtained from five methods of extraction: maceration (Neto et al, 2005) percolation (Farmacopéia Brasileira, 1988) turbolyse (De-Paris et al, 2000) reflux (Gosmann et al, 2003) and Soxhlet method (Flores et al, 2009) using three different mixture of solvents, ethanol:water (90:10 v/v), (80:20 v/v) and (50:50 v/v). For the preparation of extracts, used 10 g of P. glomerata triturated and the extract was filtered, the organic solvent was removed under "vacuum" and lyophilized.…”

Section: Crude Extractsmentioning

confidence: 99%

“…Pedersen roots extracts possess activity gastro-protector (Otofuji, 2005), anti-inflamatory, analgesic (Neto et al, 2005), antioxidant (Souza et al, 2005), anti-hiperglicemic (Sanches et al, 2001), tonic, aphrodisiac , antireumathics (Nicoloso et al, 1999), antitumoral and antidiabetics properties, and also as food supplement, among other indications (Montanari Junior, 2005). It also aids the protection and disturbances in the gastric mucous membrane , purification of the blood and the hormonal and sexual functions regulation (Guerreiro, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC

Serra¹,

Felipe²,

Cortez³

2012

Rev. bras. farmacogn.

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Pfaffi a glomerata (Spreng.) Pedersen, Amaranthaceae, is widely distributed in Brazil. Roots are considered as the world's greatest supplier and β-ecdysone is the most important compound extracted from roots of Pfaffi a glomerata. So, the aim this study was analyze the presence of β-ecdysone in the infl orescences and stems and compared with the content from roots of Pfaffi a glomerata and determine the best extractive method of β-ecdysone this plant. The crude extracts were obtained by Soxhlet method, refl ux, maceration, percolation and turbolyse. Compound extracts were quantifi ed by High Performance Liquid Chromatography (HPLC). The analysis were carried out a Phenomenex Column C18, 5 µm, 250x4,6mm, maintened at 30 o C, gradient system using as mobile phase a mixture of methanol and water, fl ow rate 1,0 mL and detection at 245 nm. Results showed Soxhlet method with ethanol:water (90:10 v/v) presented the higher concentration of β-ecdysone in P. glomerata and infl orescences showed higher amount of this active substance (3,06%), compared with stems (2,37%) and roots (1,63%), showing that the infl orescences and plant stems may also be used as a rich source of β-ecdysone.

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Section: Crude Extractsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC

Serra¹,

Felipe²,

Cortez³

2012

Rev. bras. farmacogn.

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“…Neto AG, et al 8 studied the effects of the extract of Pfaffia glomerata in different animals showing anti-inflammatory and analgesic effects similar to those they could observe with nonsteroidal drugs, like indomethacin. These authors also stated that this suggests a mechanism of action associated with the inhibition of prostaglandin synthesis, as observed in many non-steroidal drugs, because they also have among its active sitosterol, stigmasterol and allantoin.…”

Section: Introductionmentioning

confidence: 81%

A utilização da Pfaffia glomerata no processo de cicatrização de feridas da pele

Silva

Ribas-Filho

Malafaia

et al. 2010

ABCD, arq. bras. cir. dig.

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-Background -When tissue loss occurs in the skin comes the need for reconstruction and many devices and medication are proposed to increase the healing process. The use of herbal medicine, such as Pfaffia glomerata, aims to play an active role in the scar formation. Aim -To evaluate the results of the use of Pfaffia glomerata on healing process of surgical wounds in rats. Methods -A total of 40 rats, which were held surgical wounds with a punch of 3 mm in diameter at the upper right back where no substance was applied and another in the lower region, where the herbal extract was used. They were divided into four subgroups of 48 hours, one, two and three weeks in relation to sacrifice. Measures were taken on the circumference to analyze the macroscopic contraction of the wound. Microscopic data were analyzed using the hematoxylin-eosin staining to verify the inflammatory process; immunohistochemistry, factor VIII, to observe the vascular density, and Masson trichrome to study fibrosis. Results -Macroscopic plant group achieved better results than the control group. The analysis of factor VIII showed statistical significance in a week of herbal medicine. In fibrosis, it was found that during the 48 hours the control group showed 70% of cases with minimal fibrosis, whereas the plant, 90%. In one week, the control group showed 10% of cases with no fibrosis, 60% with minimal fibrosis and 30% with moderate fibrosis, while the group plant showed 70% of cases with minimal fibrosis and 30% with moderate fibrosis. In the period of two weeks, the control group maintained 60% of cases with minimal fibrosis and increased to 40% of those with moderate fibrosis, while the plant maintained its group average presented in the previous group. In three weeks both groups, control and plan, kept the percentages presented in the previous period. Conclusion -In relation to wound contraction, plant group showed better results than the control group. In regard to histological analysis, HE staining showed a greater presence of inflammation in the animals of control groups. The immunohistochemical technique showed a vascular density, higher in the plant during the period of one week and Masson's trichrome staining showed no significant changes RESUMO -Racional -Quando ocorre perda tecidual na pele surge a necessidade de reconstituir-se o tecido lesionado e atualmente cada vez mais surgem artifícios que se propõem neoformá-lo. O uso de fitoterápicos, como a Pfaffia glomerata, tem a finalidade de buscar nestes produtos princípios ativos que desempenhem efetivo papel no processo de cicatrização. Objetivo -Avaliar os resultados da utilização da Pfaffia glomerata na cicatrização de feridas cirúrgicas em ratos. Métodos -Foram utilizados 40 ratos, nos quais realizaram-se feridas cirúrgicas com punch de 3 mm de diâmetro no dorso da parte superior direita onde nenhuma substância foi aplicada e nesses mesmos animais foram também realizadas feridas cirúrgicas na região inferior, onde foi aplicado o extrato do fitoterápico. Foram divididos em...

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“…The first phase is mediated through the release of histamine, serotonin and kinins, whereas the second phase is related to the release of prostaglandin. 31 Carrageenan induced paw edema is one of the most commonly employed standard experimental model for the evaluation of anti-inflammatory drugs. It is useful in the evaluation of acute inflammation.…”

Section: Effect Of Aegle Marmelos On Carrageenan Induced Paw Edemamentioning

confidence: 99%

Analgesic and anti-inflammatory activity of ethanolic extracts of leaf and fruit pulp of Aegle marmelos in albino rats

Krishnakanth

Abhilash

Neeraja

et al. 2017

Int J Basic Clin Pharmacol

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Background: To study the analgesic and anti-inflammatory activity of the ethanolic extracts of leaf (LE) and fruit pulp (FE) of Aegle marmelos in comparison with the standard drugs pentazocine and diclofenac in albino rats. 56 healthy wistar albino rats of either sex were randomly divided into 14 groups of 4 each (n=4), weighing about 150-200grams were selected for the study. All drugs i.e. 1ml of 1% Carboxy methyl cellulose (CMC), pentazocine 10mg/kg (intraperitoneal), diclofenac 10 mg/kg and LE and FE at doses of 100mg/kg and 200mg/kg in 1% CMC (vehicle) were administered per oral one hour before the experiment.Methods: Analgesic activity was assessed by Eddy’s hot plate method (latency time) and acetic acid induced writhing (number of writhings) method. Anti-inflammatory activity was assessed by carrageenan induced hind paw edema method using digital plethysmometer.Results: Test drugs at doses LE 200mg/kg and FE 200mg/kg have shown significant increase in mean latency time in Eddy’s hot plate (P <0.001) and decrease in the average number of writhing movements (P <0.01). LE 200 and FE 200 doses did not show significant anti-inflammatory activity in Carrageenan induced paw edema model (P >0.05).Conclusions: Leaf extract (LE) and fruit extract (FE) at doses 200mg/kg have shown significant analgesic action and no anti-inflammatory action.

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Analgesic and anti-inflammatory activity of a crude root extract of Pfaffia glomerata (Spreng) Pedersen

Cited by 101 publications

References 21 publications

Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC

Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC

A utilização da Pfaffia glomerata no processo de cicatrização de feridas da pele

Analgesic and anti-inflammatory activity of ethanolic extracts of leaf and fruit pulp of Aegle marmelos in albino rats

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Analgesic and anti-inflammatory activity of a crude root extract of Pfaffia glomerata (Spreng) Pedersen

Cited by 101 publications

References 21 publications

Quantification of &#946;-ecdysone in differents parts of Pfaffia glomerata by HPLC

Quantification of &#946;-ecdysone in differents parts of Pfaffia glomerata by HPLC

A utilização da Pfaffia glomerata no processo de cicatrização de feridas da pele

Analgesic and anti-inflammatory activity of ethanolic extracts of leaf and fruit pulp of Aegle marmelos in albino rats

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Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC

Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC