Analgesic, anti-inflammatory and antioxidant properties of Buddleja globosa, Buddlejaceae

Backhouse, Nadine; Rosales, Laura Berenice Olvera; Apablaza, C.; Goïty, León; Erazo, Silvia; Negrete, Rosa; Theodoluz, C.; Rodrı́guez, Jaime A.; Delporte, Carla

doi:10.1016/j.jep.2007.11.025

Cited by 79 publications

(60 citation statements)

References 39 publications

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“…ED 50 values for Bc-Cc and Bc-Wp were 1.26 and 3.93 mg/ear, respectively, while for Indomethacin in both experiments, ED 50 was ≈1.47 mg/ear for a single topical administration in both experiments (Table 1). These results are better than data reported for B. globosa aerial parts MeOH extract evaluated for TPA model, the anti-inflammatory effect was 56.7 % inhibition at 3 mg/ear (Backhouse et al, 2008). Also, main metabolite Vb, has been evaluated previously in mice TPA model, being isolated from ethyl acetate extract of Castilleja tenuiflora.…”

Section: Anti-inflammatory and Anti-arthritic Evaluation 331 Tpa Modelcontrasting

confidence: 58%

“…These results indicated that a lesser quantity of Bc-Cc extract generated better anti-inflammatory activity than BcWp with an inhibition nearly to 50% over paw-edema development. Backhouse et al (2008), demonstrated that MeOH fraction obtained from MeOH extract of aerial parts from B. globosa Wp generated an inhibition of 61.40% on edema formation in third hour after its Manuscrito sometido a la Revista Mexicana de Ingeniería Química 1 Manuscrito sometido a la Revista Mexicana de Ingeniería Química 1 i.g. administration at dose of 600 mg/kg in Guinea pigs.…”

Section: Carrageenan Modelmentioning

confidence: 98%

“…The results showed that the aqueous and methanolic (MeOH) extracts obtained from whole wild plants (Wp) of B. globosa (Backhouse et al, 2008), B. crispa (Bukhari et al, 2016), and B. polystachia (Al-Ati et al, 2015) showed an important inhibitory effect. The inhibition was between 65 and 71%, on subplantar edema growth induced by Carrageenan in rats at doses of 50, 100, and 500 mg/kg administered by intraperitoneal (i.p.)…”

Section: Introductionmentioning

confidence: 99%

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In vivo ANTI-INFLAMMATORY ACTIVITY AND ACUTE TOXICITY OF METHANOLIC EXTRACTS FROM WILD PLANT LEAVES AND CELL SUSPENSION CULTURES OF Buddleja cordata Kunth (Buddlejaceae)

et al. 2017

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Buddleja cordata is a species used by Mexican folk medicine for treating illnesses related to inflammation such as skin wounds and arthritis. It bio-synthesizes metabolites such as verbascoside that contributes to its ethno-therapeutic properties as antiinflammatory remedy. HPLC analysis showed that the methanolic extract from cell suspension cultures (Bc-Cc) and wild plant leaves (Bc-Wp) contained verbascoside, but concentration was higher in Bc-Cc (87.48 mg/g) than in wild plant (47.34 mg/g).In the acute toxicity model, none of the extracts generated any lethality or adverse effects. In acute inflammation model induced with TPA, Bc-Cc extract showed a greater edema inhibition at 2 mg/ear (61.72%), as well for carrageenan model at 200 mg/kg (48.87%). Bc-Wp showed lesser anti-inflammatory effect in both acute inflammation models than Bc-Cc. For Adjuvant-induced arthritis both extracts at 250 mg/kg generated a moderate inhibition over edema (≈ 33%) at day 28, and they were statistically no different to phenilbutazone. The culture in suspension of B. cordata obtained by biotechnological process contains greater amount of verbascosido and showed better anti-inflammatory activity; thus, representing a source for obtaining this type of secondary metabolite of pharmacological interest. Keywords: cell suspension culture, Buddleja cordata, verbascoside, anti-inflammatory activity, median lethal dose. ResumenBuddleja cordata es una planta medicinal usada en México para el tratamiento de enfermedades relacionadas con heridas en la piel y artritis. Esta especie bio-sintetiza metabolitos secundarios como el verbascósido, el cual contribuye a sus propiedades etno-medicinales. El análisis por HPLC mostró que el extracto metanólico del cultivo en suspensión celular (Bc-Cc) y de las hojas de la planta silvestre (Bc-Wp) contienen verbascósido, siendo su concentración mayor en el cultivo Bc-Cc (87.48 mg/g) que en la planta silvestre (47.34 mg/g). Ninguno de los extractos generó letalidad o efectos adversos en el ensayo de toxicidad aguda. En el modelo de inflamación tópica inducido con TPA, Bc-Cc mostró mayor actividad antiinflamatoria; a 2 mg/oreja generó 61.72% de inhibición y en el modelo de carragenina a 200 mg/kg generó 48.87%, mientras que Bc-Wp mostró menor actividad anti-inflamatoria en ambos modelos. En el modelo de artritis inducida con Adyuvante de Freud completo, ambos extractos a 250 mg/kg mostraron moderada actividad anti-inflamatoria, con inhibición de aprox. 33% al día 28. El cultivo en suspensión celular de B. cordata obtenido por proceso biotecnológico contiene mayor cantidad de verbascosido y mostró mejor actividad anti-inflamatoria; por lo que representa una fuente para la obtención de este tipo de metabolito secundario de interés

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Section: Anti-inflammatory and Anti-arthritic Evaluation 331 Tpa Modelcontrasting

confidence: 58%

Section: Carrageenan Modelmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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In vivo ANTI-INFLAMMATORY ACTIVITY AND ACUTE TOXICITY OF METHANOLIC EXTRACTS FROM WILD PLANT LEAVES AND CELL SUSPENSION CULTURES OF Buddleja cordata Kunth (Buddlejaceae)

et al. 2017

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“…Also, phytol, which is a major compound of unsaponifiable fraction from hexane extract of C. cajan seed, was reported to inhibit histamine-, serotonin-, bradykinin-, and PGE2-induced paw edema, and to decrease TNF-a and IL-1b production in carrageenan-induced peritonitis by 48 and 43%, respectively (Silva et al, 2014). In addition, a mixture of b-sitosterol, stigmasterol, stigmastenol, stigmastanol, and campesterol reduced TPA-induced inflammation (Backhouse et al, 2008). According to Bhalke and Pal (2012), b-sitosterol exhibited a marked inhibition of carrageenan-induced rat paw inflammation by inhibiting the mediators of acute inflammation; indicating its antiinflammatory activity.…”

Section: Effect Of Inflammation On Inflammatory Markers Tumor Necrosimentioning

confidence: 99%

Assessment of anti-inflammatory, antinociceptive, immunomodulatory, and antioxidant activities ofCajanus cajanL. seeds cultivated in Egypt and its phytochemical composition

Hassan

Matloub

Aboutabl

et al. 2015

Pharmaceutical Biology

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Context: Cajanus cajan L. (Fabaceae), a food crop, is widely used in traditional medicine. Objectives: The phytochemical composition of C. cajan seeds and evaluation of the antiinflammatory, immunomodulatory, antinociceptive, and antioxidant activities were studied. Materials and methods: Unsaponifiable matter and fatty acids were analyzed by GC and GC/MS. The n-butanol fraction was chromatographed on polyamide column. The anti-inflammatory activity of hexane extract (200 and 400 mg/kg, p.o.) was evaluated using the carrageenan-induced rat paw edema at 1, 2, and 3 h. The serum tumor necrosis factor-a, interleukin-6, and immunoglobulin G levels were detected by ELISA. The hexane extract antinociceptive activity was determined by adopting the writhing test in mice. DPPH radical scavenging, total reduction capability, and inhibition of lipid peroxidation of butanol fraction were evaluated. Results and conclusion: Twenty-one unsaponifiable compounds (mainly phytol, 2,6-di-(t-butyl)-4-hydroxy-4-methyl-2,5-cyclohexadiene-1-one, b-sitosterol, stigmasterol, and campesterol), as well as 12 fatty acids (primarily 9,12-octadecadienoic and palmitic acids) were identified in hexane extract of C. cajan seeds. n-BuOH fraction contains quercetin-3-O-b-D-glucopyranoside, orientin, vitexin, quercetin, luteolin, apigenin, and isorhamnetin. For the first time, quercetin-3-O-b-D-glucopyranoside is isolated from C. cajan plant. The hexane extract (200 and 400 mg/kg) inhibited carrageenaninduced inflammation by 85 and 95%, respectively, 3 h post-carrageenan challenge. This was accompanied by an 11 and 20%, 8 and 13%, respectively, decrease of TNF-a and IL-6, as well as significant decrease in IgG serum levels. Moreover, hexane extract (200 and 400 mg/kg) decreased the number of writhings by 61 and 83%, respectively. The butanol fraction showed DPPH radical scavenging (inhibitory concentration (IC 50 ) value: 9.07 mg/ml). ARTICLE HISTORY

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“…The antioxidants present in the human diet or in plant extracts may play an important role in disease prevention (Steinmetz & Potter, 1996;Aruoma, 1998). These antioxidant properties are generally associated with phenolic compounds such as protocatechuic acid, ferulic acid, quercetin, and guavin B (Thaipong et al, 2005), but can also be attributed to the presence of other compounds such as triterpenoids (Gao et al, 2006, Backhouse et al, 2008.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and vasorelaxant activities of theTurraeanthus africanusmethanol extract

Dongmo

Dzikouk

Massoma

et al. 2009

Pharmaceutical Biology

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Analgesic, anti-inflammatory and antioxidant properties of Buddleja globosa, Buddlejaceae

Cited by 79 publications

References 39 publications

In vivo ANTI-INFLAMMATORY ACTIVITY AND ACUTE TOXICITY OF METHANOLIC EXTRACTS FROM WILD PLANT LEAVES AND CELL SUSPENSION CULTURES OF Buddleja cordata Kunth (Buddlejaceae)

In vivo ANTI-INFLAMMATORY ACTIVITY AND ACUTE TOXICITY OF METHANOLIC EXTRACTS FROM WILD PLANT LEAVES AND CELL SUSPENSION CULTURES OF Buddleja cordata Kunth (Buddlejaceae)

Assessment of anti-inflammatory, antinociceptive, immunomodulatory, and antioxidant activities ofCajanus cajanL. seeds cultivated in Egypt and its phytochemical composition

Antioxidant and vasorelaxant activities of theTurraeanthus africanusmethanol extract

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