Análise da participação da oligopeptidase B e triparedoxina peroxidase citoplasmática na virulência de &lt;i&gt;Leishmania&lt;/i&gt; (&lt;i&gt;Leishmania&lt;/i&gt;) &lt;i&gt;amazonensis&lt;/i&gt;.

Leite, Karoline Mathias

doi:10.11606/d.42.2016.tde-14042016-100555

Cited by 1 publication

(2 citation statements)

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“…Other studies have shown that genetic and phenotypic characteristics among different L. (V.) braziliensis strains are associated with different clinical manifestations and drug resistance [46,58]. Additionally, the variation among serine proteases inhibition indicate speci c traits among the conformation of their serine protease network since the speci city of PMSF, AEBSF and TLCK is different [27,54,59]. Therefore, L. (V.) braziliensis isolates can be composed of distinct enzymatic pro les that in uence host-parasite interactions and, consequently, the success or failure of speci c drugs.…”

Section: Discussionmentioning

confidence: 99%

“…Experimental evidence has demonstrated that these enzymes can act as signal peptidases; maturases of other proteins; and have a metacaspase-like activity [21,26]. Among them, oligopeptidases have important roles during infection of host macrophages [27], and subtilisins are established virulence factors in Leishmania spp. as well as in other intracellular parasites [28,29].…”

Section: Introductionmentioning

confidence: 99%

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Serine Proteases Profiles of Leishmania (Viannia) Braziliensis Clinical Isolates With Distinct Susceptibilities to Antimony

Zabala-Peñafiel¹,

Dias-Lopes²,

Cysne-Finkelstein

et al. 2021

Preprint

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Glucantime® (SbV) is the first-line treatment against leishmaniasis in South America. Its effectiveness has been associated with modulation of the parasite detoxification system that, in turn, is related to serine proteases such as subtilisins. In this study, 12 Leishmania (Viannia) braziliensis isolates from patients that presented clinical cure (Responders - R) and relapse or therapeutic failure (Non-responders - NR) were used. The parasites were assessed by in vitro susceptibility to SbIII and SbV, serine proteases activity – measured with z-FR-AMC as substrate and specific inhibitors – and expression of subtilisins and tryparedoxin-peroxidase (TXNPx). In vitro susceptibility of axenic amastigotes to SbIII showed a significant difference between R and NR groups. TLCK inhibited almost 100 % of activity in both axenic amastigotes and promastigotes while AEBSF inhibited around 70 %, and PMSF showed lower inhibition of specific isolates. Principal component and clustering analysis yielded one homogeneous cluster with only NR isolates and three heterogeneous clusters with R and NR isolates. Additionally, transcripts of subtilisins (LbrM.13.0860 and LbrM.28.2570) and TXNPx (LbrM.15.1080) were detected in promastigotes and axenic amastigotes from both groups. The data presented here show a phenotypic heterogeneity among the parasites, suggesting that exploration of in vitro phenotypes based on SbIII and serine proteases profiles can aid in the characterization of L. (V.) braziliensis clinical isolates.

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