Análise de ácidos graxos não-esterificados de plasma humano por cromatografia gasosa capilar com injeção sem divisão de fluxo

Ney, Jacqueline G.; Torres, Alexandre Guedes; Trugo, N. M. F.

doi:10.1590/s0100-40422004000400009

Cited by 7 publications

(3 citation statements)

References 14 publications

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“…Samples (1•5 ml) were injected in the gas-chromatograph, operated as follows: He gas was used as a carrier (70 kPa); the detector temperature was set at 2708C; the injector temperature was 2408C; the split ratio was 1:15; column temperature gradient was set at 2008C (held for 10 min) þ1•58C/min to 2108C (held for 20 min). NEFA were analysed by the method described in Ney et al (2004), adapted from Polette et al (1992) for splitless injections.…”

Section: Analyses Of Milk Plasma and Erythrocyte Samplesmentioning

confidence: 99%

Polyunsaturated fatty acids and conjugated linoleic acid isomers in breast milk are associated with plasma non-esterified and erythrocyte membrane fatty acid composition in lactating women

et al. 2006

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Maternal adipose tissue is a major contributor to breast milk long-chain fatty acids, probably through the pool of plasma NEFA. The fatty acid composition of the erythrocyte membrane (EM) is a biochemical index of the intake of fatty acids not synthesized endogenously and of PUFA and long-chain PUFA fatty acid status. The present study investigated the associations between breast milk fatty acid composition and the composition of plasma NEFA and of EM fatty acids with special reference to PUFA, long-chain PUFA and conjugated linoleic acid (CLA). The detailed fatty acid composition of mature breast milk was also reported. Thirty-three healthy, lactating Brazilian women donated milk samples; of these, twenty-four also donated blood samples in an observational cross-sectional study. Breast milk fatty acid composition presented several associations with NEFA and EM composition, which explained most ($50 %) of the variability of selected milk PUFA, long-chain PUFA and CLA. Milk CLA was associated with fatty acids that are markers of dairy fat intake in the diet, NEFA and EM. In general, breast milk n-3 fatty acids and CLA, but not n-6 fatty acids, were associated with EM composition, whereas both the n-6 and n-3 fatty acids and CLA in milk were associated with NEFA composition, possibly owing to its role as a direct source of fatty acids for breast milk. These findings emphasize the contribution of the NEFA pool derived from the adipose tissue to the long-chain fatty acid composition of breast milk.Fatty acid metabolism: Breast milk fatty acids: Non-esterified fatty acids: Erythrocyte fatty acids: Lactation

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Section: Analyses Of Milk Plasma and Erythrocyte Samplesmentioning

confidence: 99%

Polyunsaturated fatty acids and conjugated linoleic acid isomers in breast milk are associated with plasma non-esterified and erythrocyte membrane fatty acid composition in lactating women

et al. 2006

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“…O ácido palmítico é um ácido graxo saturado de cadeia longa formado por 16 carbonos cuja formula química é CH 3 (CH 2 ) 14 COOH. O mesmo foi escolhido como padrão no presente estudo visto que é o ácido graxo em maior concentração no plasma sanguíneo e também em muitos outros meios biológicos ou alimentos (CURI et al, 2002;NEY;TORRES;TRUGO, 2004).…”

Section: Resultsunclassified

Desenvolvimento de um método para determinação de ácidos graxos em meios biológicos utilizando a espectroscopia no infravermelho com transformada de Fourier

et al. 2011

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In the present paper a new methodology has been developed for determination of fatty acids in biological systems using FT-IR spectroscopy. For this method is not necessary chromophore reagent or pre sample preparation. Palmitic acid was chosen as standard, because it is found in several biological systems. The FT-IR spectrum of palmitic acid showed two absorption bands in the region of 2852 and 2920 cm -1 attributed to CH stretching. The results for these bands showed that the Beer-Lambert Law was followed in wide range of concentration of palmitic acid (14 to 257 mmol L -1 ). Potassium ferricyanide (K 3 [Fe(CN) 6 ]) was used as internal standard. Several interferents were tested and only cholesterol, ferric chloride (higher concentration), mixture of amino acids for the band at 2919 cm -1 (higher concentration) and triglyceride could be interferent if they appear in high concentration. Thus, this new methodology has advantage to be not expensive and simple.

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“…Some authors analyzed nonesterified fatty acids and compared two methods to determine fatty acid concentrations; one using solvent extraction followed by esterification and the other by direct esterification. (7,8) However, none of these studies compared the different methods of lipid extraction. This is the most critical step in the analysis of lipid content; it can present problems such as low recovery and fatty acid oxidation, which affect the results and consequently, the analysis of fatty acid composition, especially of polyunsaturated fatty acids (PUFA), such as the omega-3 (n-3) and omega-6 (n-6) series.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of lipid extraction and fatty acid composition of human plasma

Morais¹,

Visentainer²,

Santos³

et al. 2010

Rev. Bras. Hematol. Hemoter.

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Avaliação da extração lipídica e composição em ácidos graxos do plasma humano Aim: The objective of this study was to compare the efficiency of lipid extraction and to evaluate the fatty-acid composition in total lipids from human plasma, using a new technique and the established Folch, Lees and Stanley (FLS), Bligh and Dyer (BD), Rose-Gottlieb (RG), and Gerber (GM) methods. Method: A new technique, the alternative method, to extract total lipids using a microwave was proposed and evaluated. Results: The total lipids extracted from human plasma varied between 0.19% and 0.41%; the highest total lipid extracted were obtained by the Folch, Lees and Stanley (0.41%), alternative method (0.37%) and Rose-Gottlieb (0.36%) methods. The Gerber method was ineffective to extract total lipids from human plasma. A total of 24 fatty-acid species were quantified by gas chromatography. Of the methods studied, the highest concentrations were found using the Folch, Lees and Stanley method (p ≤ 0.05). Conclusion: The alternative method is a fast lipid-extraction technique that can be used for the identification of human plasma fatty acids, but it is not suitable for their measurement.

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Análise de ácidos graxos não-esterificados de plasma humano por cromatografia gasosa capilar com injeção sem divisão de fluxo

Cited by 7 publications

References 14 publications

Polyunsaturated fatty acids and conjugated linoleic acid isomers in breast milk are associated with plasma non-esterified and erythrocyte membrane fatty acid composition in lactating women

Polyunsaturated fatty acids and conjugated linoleic acid isomers in breast milk are associated with plasma non-esterified and erythrocyte membrane fatty acid composition in lactating women

Desenvolvimento de um método para determinação de ácidos graxos em meios biológicos utilizando a espectroscopia no infravermelho com transformada de Fourier

Evaluation of lipid extraction and fatty acid composition of human plasma

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