Análise de citocinas pela RT-PCR em pacientes com rinite alérgica

Abstract

Al lergic rhinitis is an inflammatory reaction of the nasal mucosa, in consequence of an IgE mediated hypersensitive reaction to inhaling allergens, involving different mediators and cytokine cells. Aim: The purpose of this study was to evaluate the transcriptions for IL-4, IL-5, IL-8 and IFNgama, particularly important in the nasal allergy process, especially IL-4 and IL-5. For this study we decided to evaluate atopic patients who were free from allergic crises, with the purpose of knowing the cytokine expressions during this period. Materials and Methods: Another prospective and transversal study was carried out, selecting 30 patients, 13 of these patients were pauci-symptomatic and 17 were non atopic. The groups were selected by means of a medical interview, an otolaryngologic clinical exam and allergy skin tests -Prick Test. The cytokines were investigated in fragments of the nasal mucosa, using RT-PCR -chosen because it has good reproducibility and specificity. Results: IL-5, IL-8, IFNgama cytokine values were kept homogeneous in relation to the control group. Only IL-4 presented significant statistic differences. Conclusion: Asymptomatic patients with allergic rhinitis presented with normalization of cytokine expression in the nasal mucosa, with exception of IL-4.

T cell responses to der f2 mite allergens in Thai allergic patients

“…We did not find significantly difference in IL-5 or IFN-γ gene expression from PBMC of allergic patients and healthy control subjects. This data is consistent with other investigators [21]. However, we found that IL-5 production induced by Df extract was slightly positively correlated in both allergic patients and healthy subjects.…”

“…House dust mites (HDMs) as a source of allergens and their contribution to allergic diseases, particularly asthma and rhinitis, have been recognized for many years [20]. Allergic rhinitis is an inflammatory reaction of the nasal mucosa, in consequence of an IgE mediated hypersensitive reaction to inhaling allergens, involving different mediators and cytokine cells [21]. Positive skin tests and/or serum IgE antibodies to HDM have been widely demonstrated in genetically predisposed individuals.…”