This paper aimed to analyze the influence of land use on the quality of water in Uberabinha river basin in the city of Uberlândia (MG). Using geoprocessing techniques and remote sensing data, land use, soil type, slope and hypsometric maps were drawn. Images were taken from the LANDSAT 8 satellite with 30 m space resolution, dated August 18, 2018, and from the Digital Elevation Model TOPODATA/SRTM together with the historical series data about water quality of the Uberabinha river, retrieved from Instituto Mineiro de Gestão das Águas (IGAM) and the Brazilian Ministry of Health. The data about water quality were organized according to the limits established by Conselho Nacional do Meio Ambiente (CONAMA) and international regulation. Uberabinha river basin is mostly used for agriculture. Concerning the water quality sampled from point PB 023, which was sixteen kilometers away from Uberlândia's sewage treatment plant (STP), the concentration of the variables totalscoliform index, Escherichia coli, sulfate, and phosphorus exceeded the limits set by laws between 2012 and 2018. Water quality data were collected on an annually basis, always in August. The land use influenced on the contamination of the basin water, especially, in the course that includes Uberlândia's urban area, due to a greater concentration of the parameters that indicate contamination of domestic and industrial water