Análise Morfoagronômica E Molecular Das Cultivares De Café Do Ifes Campus De Alegre

Abstract

Coffee is one of the most cultivated species and marketed the world. The morfoagronômico and molecular study is very useful for analysis of genetic diversity is important for the knowledge and use of culture. The objectives of this study were: i) perform morphoagronomic and through characterization of this estimate the genetic divergence of coffee cultivars, and ii) select the most efficient DNA extraction method and primers suitable for the molecular characterization of coffee cultivars. The survey was conducted in coffee plantations of the Coffee Park IFES Campus Alegre with eight cultivars, five Arabica (Coffea arabica L.) and three conilon (C. canephora P.). The cultivars were characterized by specific morphological descriptors for the genus Coffea. For the molecular analysis of DNA were tested five extraction protocols and after selecting the appropriate protocol, screening was performed with ten ISSR in order to ascertain the generated polymorphism. The statistical-genetic analysis of quantitative morphological characteristics were performed using the program Genes and the group obtained by the UPGMA method. For molecular analysis, ANOVA was performed of DNA concentrations followed by F test for the quantitative traits a good genetic divergence was observed. Qualitative analysis was found a low number of classes, which shows a high uniformity of the cultivars for the traits. Three DNA extraction protocols showed good DNA concentrations, and we selected five primers best electrophoretic profiles and sharper DNA bands.