In the Mediterranean basin human activity has modified landscapes for millennia, nevertheless there are few remote forest areas relatively untouched long enough from direct anthropogenic disturbance to develop old-growth attributes. The aim of this note is to assess the potential of QuickBird (QB) satellite multispectral imagery for detecting old-growth forest stands, considering as case study a Mediterranean beech forest in central Italy. The segmentation-based analysis of QB image proved to be a promising tool to detect scaledependent pattern of forest structural heterogeneity. Values of remotely sensed attributes are compared in old-growth and not-old-growth stands: the statistical analysis showed that oldgrowthness is associated to the variability of multispectral reflectance from the image objects (polygons). Green band variability, notably, expressed by Ratio_band_2 has proven to be helpful for predicting old-growthness.