Análisis de estabilidad de un sistema de fermentación acetona-butanol-etanol (ABE) a partir de glucosa empleando <i>Clostridium acetobutylicum</i> ATCC 824

Abstract

In the present study we analyzed the dynamics and stability in production of butanol by fermentation on a continuous bioreactor. The dynamic response of the system is studied as an evaluation criterion selecting the concentration of butanol obtained and using a kinetic model statistically validated, which describe the continuous cultures oscillatory behavior taking into account the influence of pH, inhibition by products and influencing parameters in the system. The bifurcation parameters chosen were the dilution rate (D) and feed substrate concentration (Sf). We used a calculation routine developed in Matlab ® and MATCONT ® to generate diagrams for dynamic behavior, steady state values and bifurcation diagrams. The results show that it is convenient to operate at smaller dilution rates (D <0.1 [h ^ (-1)]) and feed substrate concentrations (Sf) in the range of 40 to 90 [g / L] for higher concentrations of butanol. The practical implications of the phenomena discovered are analyzed in order to apply an appropriate methodology for this type of study and the implementation of it in bioprocesses.Keywords: Bifurcation, Butanol, Dynamic simulation, Nonlinear dynamics, Stability Analysis. ResumenSe analiza la dinámica y estabilidad en la producción de butanol por fermentación en un biorreactor continuo. La respuesta dinámica del sistema se estudió eligiendo como criterio de evaluación la concentración de butanol, en un modelo cinético validado estadísti-camente, que describe el comportamiento oscilatorio de cultivos continuos teniendo en cuenta la influencia del pH, inhibición por productos y los parámetros influyentes en el sistema. Se tomaron como paráme-

Unstructured kinetic model of batch fermentation of USP glycerol for lactic acid production

“…Therefore, the development of microbial kinetic models with the capacity of describing and predicting the concentration of products, biomass and substrate during the process is required (Doran, 1995). Kinetic models can also be applied in the stability analysis of fermentation (Castellanos, Matallana & López, 2014).…”