Análisis de la expresión nuclear de pSer727-STAT3 como factor pronóstico en pacientes con carcinoma renal de células claras

Lorenté, David; Arévalo, J.; Salcedo, María Teresa; Trilla, E.; Torres, Inés de; Meseguer, Anna; Moróte, Juan

doi:10.1016/j.acuro.2019.11.006

Actas Urológicas Españolas

2020

DOI: 10.1016/j.acuro.2019.11.006

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Análisis de la expresión nuclear de pSer727-STAT3 como factor pronóstico en pacientes con carcinoma renal de células claras

David Lorenté

J. Arévalo

María Teresa Salcedo

et al.

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2023

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Solamargine induces apoptosis of human renal carcinoma cells via downregulating phosphorylated STAT3 expression

Huang,

Sun,

Ren

et al. 2023

Oncol Lett

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Solamargine (SM), an active compound derived from Solanum nigrum , triggers apoptosis and inhibits the metastatic and oxidative activities of various types of tumor cells. However, the effect of SM on human renal carcinoma cells remains unknown. In the present study, the molecular mechanisms underlying the antitumor effects of SM on ACHN and 786-O cells were elucidated. Specifically, MTT and colony formation assays were conducted to evaluate the impact of SM treatment on the proliferation of ACHN and 786-O cells, and flow cytometry was conducted to determine the influence of SM on the apoptosis rates of these cells. In addition, the expression of target proteins was determined by western blotting. The results revealed that SM not only inhibited cell viability but also promoted the apoptosis of ACHN and 786-O cells in a time- and dose-dependent manner. Moreover, treatment of ACHN and 786-O cells with SM significantly enhanced the caspase-3, caspase-8 and caspase-9 activities. Furthermore, SM downregulated the expression of phosphorylated signal transducer and activator of transcription-3 (p-STAT3) and Bcl-2 but increased the expression of cleaved caspase-3, −8, −9 and Bax. BAY2353, a p-STAT3 inhibitor, inhibited the viability of ACHN and 786-O cells, increased the expression of cleaved caspase-9 and Bax and decreased the expression of p-STAT3 and Bcl-2. Further experiments demonstrated that SM inhibited tumor growth in xenograft nude mice without causing specific toxicity to the major organs. Collectively, these findings indicated that SM not only inhibited the viability but also promoted the apoptosis of ACHN and 786-O cells, through a mechanism involving downregulation of p-STAT3 expression.

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Solamargine induces apoptosis of human renal carcinoma cells via downregulating phosphorylated STAT3 expression

Huang,

Sun,

Ren

et al. 2023

Oncol Lett

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Análisis de la expresión nuclear de pSer727-STAT3 como factor pronóstico en pacientes con carcinoma renal de células claras

Cited by 1 publication

References 14 publications

Solamargine induces apoptosis of human renal carcinoma cells via downregulating phosphorylated STAT3 expression

Solamargine induces apoptosis of human renal carcinoma cells via downregulating phosphorylated STAT3 expression

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