A comparative study is presented, focusing on three different bioinspired design methodologies applied to a large-scale aeronautical tooling use case. The study aims to optimize the structure in terms of the first vibration mode, minimizing mass, and supporting operational loads. The development of lightweight metallic components is of great importance for industries such as aerospace, automotive, and energy harvesting, where weight reduction can lead to significant improvements in performance, efficiency, and sustainability. Bioinspired design offers a promising approach to achieving these goals. The study begins with an introduction to natural selection and various bioinspired concepts. It proceeds with a thorough review of the selected bioinspired design methodologies and tools, which are then applied to the chosen use case. The outcomes for each methodology were explored with respect to the design requirements. Subsequently, the most suitable design was selected according to the success criteria defined and its validation is explained. The manufacturing of this design was carried out using an advanced and novel approach specifically tailored to accommodate the large dimensions and complexity of the structure. Finally, modal testing was performed to validate the entire process, and the results obtained demonstrate the potential effectiveness of bioinspired design methodologies in achieving lightweighting and optimizing vibration modes for large-scale aeronautical tooling.