Population growth drives food demand, which requires increased production to offset up. Furthermore, the principle of sustainability must be implemented through organic farming practices to ensure that future food needs are met. However, many farmers still tend to practice conventional farming. This study aims to determine the profitability and technical sustainability level in organic, non-organic, and semi-organic rice cultivation systems in the Daerah Istimewa Yogyakarta Province. The sample was 90 farmers representing the three cultivation systems. Profitability analysis and Rice Check measurement were employed to answer the research aims. The results showed that the R/C ratio in the organic cultivation system had a better performance of 1.50 compared to the other systems of 1.36 (semi-organic) and 1.20 (non-organic). Furthermore, the three cultivation systems had higher profitability than the deposit interest rate (2.5%), which was 91.99% (organic), 74.46% (non-organic), and 70.66% (semi-organic). Rice check analysis showed that organic rice had the highest average score of 73.59% (sustainable), non-organic rice with a score of 59.27% (intermediate sustainable), and semi-organic rice with 56.39% (intermediate sustainable). This research was expected to be a reference for farmers in developing organic farming and how to conduct more efficient cultivation practices with Rice Check.