Analisis Kualitatif Fitikomia Kandungan Flavonoid Ekstrak Kayu Sepang (Caesalpinia Sappan L.) dari Ekstraksi Metode Ultrasonic Assisted Solvent Extraction

Abstract

ABSTRAK
 Penelitian ini bertujuan untuk mengidentifikasi isolat dari ekstrak kayu sepang dengan menguji keberadaan kandungan Flavonoid pada ekstrak. Isolasi dilakukan dengan melakukan ekstraksi menggunakan sonikator yaitu Ultrasonic-Assisted Solvent Extraction dengan menggunakan pelarut organik yaitu Etanol. Proses etanolik ini digunakan untuk mengisolasi senyawa flavonoid pada kayu sepang dengan menggunakan konsentrasi pelarut yang optimum dengan variasi konsentrasi pelarut 85%, 90%, 95%, dan 99% serta variasi lama waktu ekstraksi sonikasi yaitu 15 menit, 20 menit, dan 25 menit. Hasil ekstraksi akan dikarakterisasi menggunakan Gas Chromatography Mass Spectrometry (GCMS) dengan terlebih dahulu dikarakterisasi awal melalui kadar air, kadar abu, serta uji kualitataif dari ekstrak. Hasil yang diperoleh adalah kadar air dan kadar abu masing-masing adalah 6% dan 98%. Hasil uji kualitatif menunjukkan bahwa terjadi perubahan warna ekstrak yang mencirikan bahwa terdapatnya senyawa Flavonoid pada ekstrak kayu sepang. Ekstrak yang mendekati larutan kontrol adalah pada konsentrasi 90% dimana perubahan warna terjadi menjadi merah keunguan sama dengan larutan kontrol (Kuersetin).
 
 Kata kunci: Kayu Sepang, UASE, Brazilin
 
 ABSTRACT
 This study aims to identify isolates from sappan wood extracts by examining the presence of Flavonoid content in the extract. Isolation is done by extraction using sonicator that is Ultrasonic-Assisted Solvent Extraction by using organic solvent that is Ethanol. This ethanolic process is used to isolate flavonoid compounds in sappan wood by using optimum solvent concentration with 85%, 90%, 95%, and 99% solvent concentration variations and variation of time of sonication extraction ie 15 minutes, 20 minutes, and 25 minutes. The extraction results will be characterized using Gas Chromatography Mass Spectrometry (GCMS) by first characterized by water content, ash content, and qualitative test of the extract. The results obtained are water content and ash content are 6% and 98%. The result of qualitative test indicate that there is a change of color of extract which characterizes that the presence of Flavonoid compound in the extract of sappan wood. The extracts close to the control solution were at a 90% concentration where the color change occurred to purplish red as well as the control solution (Kuersetin).
 
 Keywords: Kayu Sepang, UASE, Brazilin

Potential of Secang Wood (Caesalpinia sappan L.) Ethanol Extractas Anti-Oxidant and Sun-Protection

“…Pertamawati et al, (2017) in her research on secondary metabolite content in sappan wood also found the presence of phenolics, triterpenoids, flavonoids, and alkaloids. Flavonoids were discovered in secang wood extracted using an ultrasonic wave assisted extraction method (Asfar & Yasser, 2019). Terpenoids, flavonoids, and phenolics are secondary metabolites discovered in wood ethanol extract cups.…”

A Comprehensive Review on Bioactive Compounds Found in Caesalpinia sappan

“…The results show that the polyphenol content of sappan wood at three pH values was 34.33% (pH 6), 13.70% (pH 7), and 12.66% (pH 8). The analysis findings show that pH 6 has the highest polyphenol level and that as pH increases, polyphenol content decreases [23].…”

Avicennia Marina: A Natural Resource for Male Anti-Fertility in Family Planning

“…As an angiosperm plant, A. marina has a wide variety of flavonoids. The content of flavonoids in A. marina is much higher than several species of plants which are indicated to have potential as male anti-fertility agents, including Caesalpinia sappan which contains only one type of flavonoid [53]; Piper ornatum has three types of flavonoids [52]; and durian rind (Durio zibethinus) has nine types of flavonoids [48].…”