Analisis Pola Pertumbuhan dan Pendekatan DNA Barcoding untuk Identifikasi Turbo stenogyrus P. Fischer, 1873 (Mollusca: Gastropoda)

Saleky, Dandi; Leatemia, Simon P.O; Pattiasina, Thomas Frans; Isma, Isma; Pangaribuan, Rosa D.; Welliken, Marius Agustinus; Melmambessy, Edy H.P.; Dailami, Muhammad

doi:10.21776/ub.biotropika.2020.008.02.03

Cited by 10 publications

(11 citation statements)

References 7 publications

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“…A high bootstrap value determines the stability of the formed tree formation. The higher the bootstrap value, the better the grouping and branching formed [32]. The results of the phylogenetic tree analysis showed that high bootstrap values were found in group three with a bootstrap value category of 99%.…”

Section: Nucleotide Compositionmentioning

confidence: 95%

Relationship analysis of Scalloped Hammerhead (Sphyrna lewini) from West Aceh Waters using molecular genetics approach

Bahri

Hikmah²,

Fadli³

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Hammerhead sharks are particularly vulnerable to fishing because of their slow growth rate and reproductive constraints. This type of sharks needs special attention due to its conservation status. This research was aimed to describe the relationships of the hammerhead sharks from West Aceh waters with the hammerhead sharks from other places. Samples of the shark were collected from Ujong Baroh Fish Port, West Aceh. The samples were analysed at the Laboratory of Department of Marine Science and Fisheries, Syiah Kuala University. The laboratory activity was started with extraction of DNA enhanced by Polymerase Chain Reaction method and visualized by an electrolytical. Analysis using BLAST showed that the first sample obtained 94%-95% cover query value and produced a 683 base pair length while the second sample obtained 98% per sample for a cover query value and produced a 658 base pair length. The average nucleotide values of the hammerhead shark were T=32.8, C=26.6, A=25.7 and G=14.9. The phylogenetic tree shows that the hammerhead sharks from the study area were more similar to the hammerhead sharks from Australia and Malaysia. Overall, based on the nucleotide composition, phylogenetic tree and genetic distance, the hammerhead sharks from the study area are unique compared to the populations from the other places.

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Section: Nucleotide Compositionmentioning

confidence: 95%

Relationship analysis of Scalloped Hammerhead (Sphyrna lewini) from West Aceh Waters using molecular genetics approach

Bahri

Hikmah²,

Fadli³

2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…Analisis kekerabatan, sejarah evolusi maupun dinamika evolusi dapat dipelajari melalui rekonstruksi filogenetik (Sohpal, 2013;Rumanta et al, 2020). Pendekatan filogenetik molekuler telah banyak dilakukan dalam mengidentifikasi spesies ikan (Jefri et al, 2015;Saleky et al, 2020;Dailami et al, 2021). Filogenetik molekuler dengan DNA mitokondria dapat membedakan dan mengidentifikasi spesies yang secara morfologi sulit dilakukan .…”

Section: Pendahuluanunclassified

“…Melimpahnya sumberdaya ikan Oreochromis spp. di alam membuat identifikasi molekuler menjadi sangat penting untuk dilakukan (Layton et al, 2014) karena identifikasi dengan pendekatan DNA barcoding dapat membedakan spesies secara cepat dan akurat (Saleky et al, 2020). Belum adanya studi tentang DNA barcoding dan keragaman genetik ikan Oreochromis spp.…”

Section: Pendahuluanunclassified

Kajian Molekuler Ikan Oreochromis spp. dari Perairan Daratan Merauke-Papua, Berdasarkan DNA Mitokondria Fragmen Gen Sitokrom Oksidase Subunit I

Saleky

Sianturi

Dailami

et al. 2021

JFS

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Pemanfaatan perikanan secara lestari sangat diperlukan agar sumberdaya perikanan yang ada saat ini bisa terus dimanfaatkan dan juga dalam rangka pemulihan stok perikanan yang telah rusak. Ikan nila (Oreochromis niloticus) dan ikan mujair (Oreochromis mossambicus) secara luas telah dikenal sebagai salah satu spesies ikan air tawar yang penting dalam perikanan budidaya. Penelitian ini dilakukan dengan tujuan identifikasi spesies ikan Oreochromis spp. dari perairan daratan Merauke dengan menggunakan marka gen COI. Amplifikasi DNA dengan gen COI menghasilkan panjang sekuen DNA 656 bp dengan hasil identifikasi adalah O. niloticus dan O. mussambicus dengan tingkat kemiripan 100 % pada masing-masing spesies. Berdasarkan analisis filogenetik dan jarak genetik, sekuen DNA yang dianalisis membentuk clade sesuai dengn hasil indentfikasi dengan jarak genetik 0,000 - 0,002 pada ikan nila dan 0,000 pada ikan mujair. Analisis keragaman genetik ditemukan hanya 1 haplotype dari kedua jenis ikan tersebut. DNA barcode dan filogenetik dapat digunakan dalam mengidentifikasi spesies dalam pengelolaan dan konservasi.

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“…Nilai bootstrap yang tinggi menentukan kestabilan pembentukan pohon yang terbentuk. Semakin tinggi nilai bootstrap, maka semakin baik pengelompokkan dan percabangan yang terbentuk (Saleky et al, 2020).…”

Section: Pohon Filogenetikunclassified

Identifikasi dan Analisis Filogenetik Ikan Ekonomis Penting Oreochromis sp. dengan Pendekatan DNA Barcoding

Sianturi

Dailami²,

Saleky³

2021

B : JIB

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Species identification is one of the most basic and important factors in fisheries management. Oreochromis sp., such as tilapia (Oreochromis mosambicus) and tilapia (Oreochromis niloticus) are introduced species of fish originating from outside Merauke which are now the dominant fish and are commonly found in inland waters of Merauke. The purpose of this study was to identify the economically important fish species Oreochromis sp. which landed at Merauke Traditional Market with DNA Barcoding approach. DNA amplification with the COI gene resulted in a DNA sequence length of 655 bp with the identification result being Tilapia (Oreochromis niloticus). Based on the phylogenetic tree data and genetic distance, the DNA sequences of the samples formed the same clade as the Oreochromis niloticus sequences from GeneBank with 0% genetic distance and it was estimated that these species came from the same parents. Identification with DNA Barcoding makes it easy to identify species quickly and accurately.

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Analisis Pola Pertumbuhan dan Pendekatan DNA Barcoding untuk Identifikasi Turbo stenogyrus P. Fischer, 1873 (Mollusca: Gastropoda)

Cited by 10 publications

References 7 publications

Relationship analysis of Scalloped Hammerhead (Sphyrna lewini) from West Aceh Waters using molecular genetics approach

Relationship analysis of Scalloped Hammerhead (Sphyrna lewini) from West Aceh Waters using molecular genetics approach

Kajian Molekuler Ikan Oreochromis spp. dari Perairan Daratan Merauke-Papua, Berdasarkan DNA Mitokondria Fragmen Gen Sitokrom Oksidase Subunit I

Identifikasi dan Analisis Filogenetik Ikan Ekonomis Penting Oreochromis sp. dengan Pendekatan DNA Barcoding

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