Análisis preliminar de la amplificación de microsatélites en abejorros para estudios de biodiversidad y conservación

Cejas, Diego; Gallego, Carlos; Gabaldón, Irene Muñoz; Tarín, P. de la Rúa

doi:10.21071/az.v68i263.4204

Cited by 2 publications

(1 citation statement)

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“…Ten microsatellite loci designed for B. terrestris 51,62 were amplified in all individuals with two multiplex sets following Cejas et al 63 : RB1 (B10, B11, B100, B96, B124 and B126), and RB2 (B118, B119, B121 and B132). PCR products were sent to the Servei Central de Suport a la Investigació Experimental (University of Valencia, Spain) for capillary electrophoresis on an ABI Prism 3700 (Applied Biosystems).…”

Section: Microsatellite Amplification and Sample Genotypingmentioning

confidence: 99%

Spatial and temporal patterns of genetic diversity in Bombus terrestris populations of the Iberian Peninsula and their conservation implications

Cejas

Rúa

Gallego

et al. 2021

Sci Rep

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The bumblebee Bombus terrestris is used worldwide for crop pollination. Despite its positive impact on crop yield, it has become a widespread threat to biodiversity due to its interactions with local bumblebee populations. Commercial subspecies introduced to the Iberian Peninsula since the 1990s without any regulation have colonized the environment, with evidence of naturalization and introgression with the endemic subspecies Bombus terrestris lusitanicus. We have used mitochondrial and nuclear genetic data to describe the current genetic diversity of the Iberian population and to estimate the expansion of commercial bumblebees. Samples from the natural distribution range of the commercial subspecies, the natural intergradation area between the two subspecies and from a period prior to the use of commercial colonies (i.e., before the 1990s) have been used for comparison. Our results show that the mitochondrial haplotype of the commercial breeds has spread throughout the territory, which, together with subtle changes observed in the nuclear genetic diversity of the populations, indicates that hybridization and consequent introgression are occurring in most of the peninsula. It is, therefore, necessary to improve the existing legislation concerning the management and exportation of commercial bumblebees to conserve locally adapted populations.

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Section: Microsatellite Amplification and Sample Genotypingmentioning

confidence: 99%