Analog and simulated microgravity platforms for life sciences research: Their individual capacities, benefits and limitations

Oluwafemi, Funmilola Adebisi; Neduncheran, Adhithiyan

doi:10.1016/j.asr.2022.01.007

Cited by 15 publications

(9 citation statements)

References 19 publications

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“…Due to the fact that studying the effects of real microgravity (in space) is cost intensive and limited in terms of manual human handling and sample numbers, approaches which simulate microgravity on Earth are established. Earth-based methods to study microgravity effects are becoming more widely used and include drop towers, parabolic flights, and simulated microgravity (sμG) platforms, like rotating wall vessels (RWVs) and the random positioning machine (RPM) [7][8][9]. Using these methods, there have been advances in understanding how microgravity and sμG affect physiological and pathological processes [10,11].…”

Section: Introductionmentioning

confidence: 99%

3D microenvironment attenuates simulated microgravity-mediated changes in T cell transcriptome

ElGindi

Sapudom

Laws

et al. 2022

Cell. Mol. Life Sci.

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Human space travel and exploration are of interest to both the industrial and scientific community.However, there are many adverse effects of spaceflight on human physiology. In particular, there is a lack of understanding to the extent by which microgravity affects the immune system. T cells, key players of the adaptive immune system and long term immunity, are present not only in blood circulation, but also reside within tissue. As of yet, studies investigating the effects of microgravity on T cells are limited to peripheral blood or traditional 2D cell culture that recapitulates circulating blood. To better mimic interstitial tissue, 3D cell culture has been well established for physiologically and pathologically relevant models. In this work, we utilize 2D and 3D cell culture to gain an understanding of how simulated microgravity affects both circulating and tissue resident T cells. T cells were studied in both resting and activated stages. We found that 3D cell culture attenuates the effects of simulated microgravity on the T cells transcriptome and nuclear irregularities compared to 2D cell culture. Interestingly, simulated microgravity appears to have less effect on activated T cells compared to those in the resting stage. Overall, our work provides novel insights into the effects of simulated microgravity on circulating and tissue resident T cells which could provide benefits for the health of space travelers.

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Section: Introductionmentioning

confidence: 99%

3D microenvironment attenuates simulated microgravity-mediated changes in T cell transcriptome

ElGindi

Sapudom

Laws

et al. 2022

Cell. Mol. Life Sci.

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“…Once it adapts to this environment, reducing the gravity value to normal earth gravity and investigating the response generated by the two different acceleration levels [113]. The result could be the same as the microgravity response [114]. However, to implement this paradigm, the cell culture system needs to be sturdy at the hypergravity level.…”

Section: Plausible Laboratory-based Microgravity Simulatorsmentioning

confidence: 99%

Lab-On-a-Chip Technologies for Microgravity Simulation and Space Applications

Vashi¹,

Sreejith²,

Nguyen³

2022

Preprint

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Gravity played an important role in the development of life on earth. The effect of gravity on a living organisms can be investigated by controlling the magnitude of gravity. Most reduced gravity experiments are conducted on the lower earth orbit (LEO) in the International Space Station (ISS). However, running experiments in ISS face challenges such as high cost, extreme condition, lack of direct accessibility, and long waiting period. Therefore, researchers have developed various ground-based devices and methods to perform reduced gravity experiments. However, the search for life outside the earth requires more intensive research. Advancements in conventional methods and the development of new tools are required for this purpose. The advantages of Lab-on-a-Chip (LOC) devices make them an attractive option for simulating microgravity. This paper briefly reviews the advancement of LOC technologies for simulating microgravity in a earth-based laboratory.

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“…Micromachines 2022, 13, x FOR PEER REVIEW 12 of 20 the gravity value to normal earth gravity and investigating the response generated by the two different acceleration levels [116]. The result could be the same as the microgravity response [117]. However, to implement this paradigm, the cell culture system needs to be sturdy at the hypergravity level.…”

Section: Plausible Laboratory-based Microgravity Simulatorsmentioning

confidence: 99%

Lab-on-a-Chip Technologies for Microgravity Simulation and Space Applications

2022

View full text Add to dashboard Cite

Gravity plays an important role in the development of life on earth. The effect of gravity on living organisms can be investigated by controlling the magnitude of gravity. Most reduced gravity experiments are conducted on the Lower Earth Orbit (LEO) in the International Space Station (ISS). However, running experiments in ISS face challenges such as high cost, extreme condition, lack of direct accessibility, and long waiting period. Therefore, researchers have developed various ground-based devices and methods to perform reduced gravity experiments. However, the advantage of space conditions for developing new drugs, vaccines, and chemical applications requires more attention and new research. Advancements in conventional methods and the development of new methods are necessary to fulfil these demands. The advantages of Lab-on-a-Chip (LOC) devices make them an attractive option for simulating microgravity. This paper briefly reviews the advancement of LOC technologies for simulating microgravity in an earth-based laboratory.

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Analog and simulated microgravity platforms for life sciences research: Their individual capacities, benefits and limitations

Cited by 15 publications

References 19 publications

3D microenvironment attenuates simulated microgravity-mediated changes in T cell transcriptome

3D microenvironment attenuates simulated microgravity-mediated changes in T cell transcriptome

Lab-On-a-Chip Technologies for Microgravity Simulation and Space Applications

Lab-on-a-Chip Technologies for Microgravity Simulation and Space Applications

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