Analog Computation of Electrophoresis Patterns

Abstract

Instrumentation is described for the computation of stained paper electrophoresis patterns of serum proteins. The unit not only plots the dyed protein fractions, but with its electronic units, stores the individual integration voltages, scales the voltages to a percentage of the total, and plots the data on a bar graph. By including the total protein value obtained by chemical analysis, a second survey of the integrator voltages provides the concentrations of the protein fractions in grams per 100 ml. of serum.

Direct-Reading, Response-Compensating Integrator for Gas Chromatography.

“…Applications of this instrument for densitometry in paper and thin layer chromatography and electrophoresis may also be found. The work of Seligson, et al (6) illustrates the application of a similar system to electrophoresis. The system described in that work requires a separate amplifier for each chromatographic peak being integrated.…”

Biochemical Analysis

“…A theory of zone electrophoresis (78) and a mechanism of electrophoretic migrations in paper (173) were contributions to the theory of electrophoresis. New instrumentation included a means of analog computation of electrophoresis patterns (296), improvements in the resolving power of free flow electrophoresis (264), and a means of separating subcellular particles by density-gradient electrophoresis (116). The theoretical basis for this last analytical procedure was also discussed.…”