Analog Non-Coherent Spectrozonal Lens-less Microscopy of Hydra sp. (Phylum Cnidaria, Class Hydrozoa) with combined Raster Scan and Cross-Band Shifting

Abstract

This article discusses a new microscopic research technique for invertebrate zoology and hydrobiology. The proposed method is based on the principles of lensless microscopy, carried out in an analogous version (including in the presence of digitization at the peripheral link) using monochrome devices with charge coupled and wavelength tuning at the source level. Previously tested by the author options using tubes using an internal photoelectric effect such as vidicon/plumbicon, as well as tubes using an external photoeffect such as superorticon and others, were not considered as a worthy alternative to semiconductor matrix CCD detectors, due to the lack of resolvometric characteristics, according to the criteria of the cellular resolution of the majority hydrobiological objects, as well as by virtue of the "black orthicon halo" effect in the irradiated ROI ("black orthicon halo"), which displaces the frequency-contrast function of the detectors, caused by excessive emission of photoelectrons, leading to the accumulation of charge. The article presents some of the oldest data obtained personally by the author on an independently assembled experimental setup (CCD), available for assembly by a layman; therefore, the article can also be considered as a guide to DIY-making appropriate installations for zoological applications. At the moment, installations of this kind can also be used in conjunction with laser sources (for example, diode pumped lasers, DPSSL) and polarization components of the optical path. When using standard means of analog video recording, it is possible to use a time, address-time code embedded in the register, including in time-lapse mode, which is convenient for dynamic zoological research. And in the case of digital recording with a variable flow rate, a similar code will allow using highspeed modes with a time resolution down to microseconds.