Analogs of Firefly Luciferin

Firefly Luciferase Produces Hydrogen Peroxide as a Coproduct in Dehydroluciferyl Adenylate Formation

“…As it cannot be adenylated by Luc [31] it is not a bioluminescent substrate, [32] and since adenylation is a prerequisite for the side oxidation under discussion, no H 2 O 2 was expected to be formed with this analogue. The results obtained fully confirmed our predictions (Figure 4).…”

Proluciferin Acetals as Bioluminogenic Substrates for Cytochrome P450 Activity and Probes for CYP3A Inhibition

“…Numerous structure/activity articles (White et al, 1965;White and Woerther, 1966;Branchini et al, 1989;Woodroofe et al, 2008) have outlined the structural requirements of bioluminescent luciferase substrates. In contrast to a bioluminescent substrate, a bioluminogenic substrate is a luciferase prosubstrate that does not produce light with luciferase but can be converted to a light-generating bioluminescent substrate by an enzyme or system of interest.…”

Synthetic Reactions in PEG: PEG-Assisted Synthesis of 2-Cyano-6-methoxybenzothiazole, A Key Intermediate For The Synthesis of Firefly Luciferin

“…The residue was purified by sublimation under reduced pressure (40°C) to give colorless needles (5; 1.670 g, 83.5%), mp 52.0-53.0°C (lit. 13 ) mp 52.5-53.0°C, 30% yield). The IR and IH-NMR spectra were identical to those of an authentic sampleY) ElMS m/z: 201 (M+, 35%) and 199 (M +, 100%); Beilstein test, positive.…”