Analyse de la CDT par électrophorèse capillaire: comparaison de deux analyseurs

Grandjean, F.; Rodenbach, Marie-Pierre; Legros, F.; Nuyens, Vincent; Cirriez, J.-M.; l’Escaille, François de; Vankerkhoven, P.

doi:10.1016/j.immbio.2007.08.003

Cited by 3 publications

(2 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cutoff used for the Helena V8 assay is 1.60% [86] whereas the cutoff values for the CEofix assays and the Verona assay are 1.8 or 1.7% [4,81,82,85]. Overall, there is high correlation between data obtained by the different CZE assays and for the comparison of CZE data to those obtained by HPLC [30, 42,87,107,[109][110][111][113][114][115][116][117][118][119][120][121][122][123][124][125]. A detailed comparison of most of these data is given in [4].…”

Section: Monitoring Of Cdtmentioning

confidence: 99%

Monitoring of transferrin isoforms in biological samples by capillary electrophoresis

Caslavska

Thormann

2017

J of Separation Science

View full text Add to dashboard Cite

Work dealing with the monitoring of transferrin isoforms in human serum and other body fluids by capillary electrophoresis is reviewed. It comprises capillary zone electrophoresis and capillary isoelectric focusing efforts that led to the exploration and use of assays for the determination of carbohydrate‐deficient transferrin as a marker for excessive alcohol intake, genetic variants of transferrin, congenital disorders of glycosylation and β‐2‐transferrin, which is a marker for cerebrospinal fluid leakage. This paper provides insight into the development, specifications, strengths, weaknesses, and routine use of the currently known capillary electrophoresis based assays suitable to detect transferrin isoforms in body fluids. The achievements reached so far indicate that capillary zone electrophoresis is an attractive technology to monitor the molecular forms of transferrin in biological specimens as the assays do not require an elaborate sample pretreatment and thus can be fully automated for high‐throughput analyses on multicapillary instruments. Assays based on capillary isoelectric focusing are less attractive. They require immunoextraction of transferrin from the biological matrix and mobilization after focusing if instrumentation with a whole‐column imaging detector is not available. Interactions of the carrier ampholytes with the iron of transferrin may prevent iron saturation and thus provide more complicated isoform patterns.

show abstract

Section: Monitoring Of Cdtmentioning

confidence: 99%

Monitoring of transferrin isoforms in biological samples by capillary electrophoresis

Caslavska

Thormann

2017

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…dilution of the serum with an Fe (III) containing reagent, CZE‐based CDT assays do not require any sample preparation. Routine methods with their cut‐off values are listed in Table , whereas comparative CDT data determined with CZE and other assays are summarized in Table . CDT routine analyses by CZE are performed on (i) the well distributed automated multicapillary platforms Capillarys 1, Capillarys 2, and MINICAP (that feature eight, eight, and two capillaries, respectively) of Sebia (Evry, France), and (ii) single capillary P/ACE instruments of Beckman Coulter (models 2200, 5500, MDQ, PA‐800; Fullerton, CA, USA) using the CEofix CDT reagents of Analis (Suarlée, Belgium) or homemade reagents (Verona assay).…”

Section: Introductionmentioning

confidence: 99%

Monitoring of alcohol markers by capillary electrophoresis

Caslavska

Thormann

2012

J of Separation Science

View full text Add to dashboard Cite

Work dealing with the monitoring of alcohol markers by CE performed during the past two decades led to the development of assays for carbohydrate-deficient transferrin (CDT), ethyl sulfate, ethyl glucuronide, and phosphatidylethanol in body fluids and first attempts for the detection of the urinary 5-hydroxytryptophol/5-hydroxyindoleacetic acid ratio and stable hemoglobin acetaldehyde adducts. Most notably are assays for CDT that have been commercialized and are being used in many laboratories under routine conditions. This paper provides insight into the development, specifications, and use of the currently known CE-based assays suitable to detect alcohol markers. The achievements reached so far indicate that CE is an attractive technology for monitoring alcohol markers. This is particularly seen with the CDT assays that do not require an elaborate sample pretreatment and thus could be fully automated for high-throughput analyses on multicapillary instruments.

show abstract