Sedimentary rock samples from Jebel Chaker in the eastern part of the Ypresian basin in central-northern Tunisia were analyzed using various geochemical methods in order to decipher their organic signature. Examination of the distribution of total organic carbon (1.04 -1.82%) suggests that the petroleum potential of Ypresian facies is not ignored in such area. The Ypresian episode permitted the accumulation of organic matter, which is typically marine plankton as indicated by the unimodal distribution of N -alkane at n C 18 and by the predominance of the aliphatic hydrocarbons compared to the aromatics. It is concluded that the Ypresian organic matter is relatively immature, as indicated by the high content of polar compounds (3 -67%) in bitumen. This conclusion is supported by the relative low T max values (433 -438°C), suggesting that the organic matter is located towards the end of diagenesis and beginning of catagenesis. These new results testify to the establishment of suboxic conditions that led to the accumulation and preservation of good quantities of organic matter in central-northern Tunisia during the Ypresian. Due to their geochemical characteristics, the Chaker facies represent new potential source rocks in central-northern Tunisia.