Analyses of Indole Compounds in Sugar Cane (Saccharum officinarum L.) Juice by High Performance Liquid Chromatography and Liquid Chromatography-Mass Spectrometry after Solid-Phase Extraction

Abstract

Simultaneous quantitative analysis of 10 indole compounds, including indole-3-acetic acid (IAA, one of the most important naturally occurring auxins) and some of its metabolites, by high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) after solid-phase extraction (SPE) was reported for the first time. The analysis was carried out using a reverse phase HPLC gradient elution, with an aqueous mobile phase (containing 0.1% formic acid) modified by methanol. Furthermore, a novel SPE procedure was developed for the pre-concentration and purification of indole compounds using C 18 SPE cartridges. The combination of SPE, HPLC, and LC-MS was applied to screen for the indole compounds present in sugar cane (Saccharum officinarum L.) juice, a refreshing beverage with various health benefits. Finally, four indole compounds were successfully detected and quantified in sugar cane juice by HPLC, which were further unequivocally confirmed by LC-MS/MS experiments operating in the multiple reaction monitoring (MRM) mode.

Production of Indole Acetic Acid by a Wood Degrading Fungus Phanerochaete chrysosporium

“…A peak comparable to standard IAA confirmed the presence of IAA in the methanolic extract (Figure 4). However, an adjacent peak was also observed, which might belong to some related indole compound (indole-3-carboxylic acid) or amino acid conjugate (IAA-Ala) as described earlier during the HPLC determination of IAA [22,23]. Similarly, HPLC analysis of IAA produced by fungus Fusarium delphinoides showed the comparable peaks to present study [24].…”

Assessing anticancer, antidiabetic, and antioxidant capacities in green-synthesized zinc oxide nanoparticles and solvent-based plant extracts

“…A good understanding of these phytochemicals contributes positively to a better understanding of plants' therapeutic potential [ 29 ,[ [26] , [27] , [28] , [30] ]. Biochemical compounds can be obtained from different of the plant parts, such as roots, leaves, stem, bark, flowers, fruits and seeds [ [26] , [27] , [28] , [30] , [31] , [32] ,].…”