Analyses of several seed viability markers in individual recalcitrant seeds of <i>Eugenia stipitata</i> McVaugh with totipotent germination

Calvi, Geângelo Petene; Aud, F. F.; Ferraz, Isolde Dorothea Kossmann; Pritchard, Hugh W.; Kranner, Ilse

doi:10.1111/plb.12466

Cited by 26 publications

(21 citation statements)

References 35 publications

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“…Another important characteristic is that, in addition to the single embryonic axis, which is microscopic (Justo et al, 2007), Journal of Seed Science, v.40, n.3, p.221-236, 2018 these seeds are able of producing new roots and even entire seedlings from cotyledon cells (Anjos and Ferraz, 1999;Calvi et al, 2016), a characteristic rarely found in other species (Malik et al, 2005;Joshi et al, 2006;Asomaning et al, 2011). However, it is rare the formation of more than one root or seedling from a single seed at the same time.…”

Section: Strategies To Ensure the Perpetuation Of The Species With Rementioning

confidence: 99%

A new approach towards the so-called recalcitrant seeds

Barbedo

2018

J. Seed Sci.

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Water is essential, irreplaceable, and indispensable for any kind of carbon-based-life metabolic activity. Water-dependent living beings are the expected pattern in nature. However, some organisms can survive for some time at a minimum water content, such as seeds of some species (orthodox seeds). Nevertheless, the expected standard life behavior is found in seeds of another group of species, the so-called recalcitrant seeds, which are sensitive to desiccation. A huge range of different behaviors can be found between these two groups, leading authors to consider that orthodoxy and recalcitrance is not an all-or-nothing situation. Notwithstanding, we are still too far from understanding the differences and similarities between all these kinds of seeds and this has been a serious barrier to the development of plant conservation technologies. A new approach to understanding the differences between these seeds is presented here based on seed maturation, environmental influences, and evolution. From this point of view, all kinds of seed behavior are contemplated and, consequently, some new perspectives are considered for the recalcitrant seed conservation technology, the most intensely desired technology nowadays in this area.

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Section: Strategies To Ensure the Perpetuation Of The Species With Rementioning

confidence: 99%

A new approach towards the so-called recalcitrant seeds

Barbedo

2018

J. Seed Sci.

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“…The seeds from both lots were submitted to a pre-germination treatment, where the fibrous seed coat was removed from the radicle protrusion region and at the opposite end of the seed (Calvi et al, 2017).…”

Section: Germination Testsmentioning

confidence: 99%

“…Glutathione analysis, viability staining and determination of seed moisture content After flooding, seeds of Lot 2 were decoated, and seed quarters were used for viability staining, seed MC determination, and analysis of GSH and GSSG in each individual seed (Calvi et al, 2017). Two quarters were immediately frozen in liquid nitrogen and freezedried for 3 days to prevent redox reactions prior to analysis.…”

Section: Germination Testsmentioning

confidence: 99%

Exceptional flooding tolerance in the totipotent recalcitrant seeds ofEugenia stipitata

et al. 2017

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Eugenia stipitata occurs along rivers in Western Amazonia and produces berry-type fruits with economic potential. Its large recalcitrant (i.e. desiccation-intolerant) seeds have been proposed as a model to study seed stress response, as no apparent differentiation between the embryonic axis and the fused cotyledons are visible. Here, the longevity of submerged seeds was analysed with a view to understanding adaptive mechanisms to seasonal flooding. Submerged seeds began germinating after 2 months. After 1 year, 87 and 96% total germination was reached when seeds were submerged under a water column of 6 cm (where seedlings could emerge from under the water) and 26 cm (where seedlings could not reach the water surface), respectively. Seedling morphology was altered underwater, with short internodes and rudimentary leaf blades, and when submersion was terminated, seedlings transplanted to nursery conditions recovered a normal phenotype. Furthermore, when seedlings were detached from the seeds, the 'resown' seeds produced a second, normal seedling within 9 months. Concentrations of the antioxidant glutathione, which was measured as a stress marker, increased with submersion time in water. Seeds that had developed roots and shoots underwater had higher concentrations of glutathione disulphide than non-germinated seeds, suggesting that the flooding stress was more intense for seedlings than seeds, although more oxidizing cellular redox environments are also consistent with the conditions required for differentiation. Submergence underwater is recommended for storage of the recalcitrant seeds of E. stipitata for up to 1 year.

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“…Studies performed with cuttings of Eugenia spp. seeds showed that more than one plant can be obtained from one seed (Silva et al, 2005;Amador and Barbedo, 2011;Teixeira and Barbedo, 2012;Prataviera et al, 2015;Calvi et al, 2016). It was also found that dependence on cuttings for development of these tissues indicates that injuries in seeds may initiate some process of inducing formation of new roots and seedlings, or they may block the autoinhibition of these formations in seeds (Delgado and Barbedo, 2011;Amador and Barbedo, 2015).…”

Section: Introductionmentioning

confidence: 99%

Cutting and regeneration of roots and seedlings from seeds of Eugenia candolleana DC. at different maturity stages

et al. 2019

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The potential to generate new roots and even whole plants even after the removal of most of their reserves is a typical characteristic of monoembryonic seeds of species of the genus Eugenia. Previous studies have shown that seeds of Eugenia can produce seedlings even with only ¼ of their mass, including seeds at the initial stages of maturity or seeds already in germination. This could be an important ecologically strategy for ensuring propagation of Eugenia species since they would be able to develop serial seedlings from the same seed, depending on environmental conditions, but this has not yet been evaluated. In this study, seeds of E. candolleana at four maturity stages were cut longitudinally or transversally, and they maintained high germination percentages and production of normal seedlings. In a second experiment, the first and second seedlings that developed were removed and regeneration capacity was also evaluated. The results showed that it was possible to obtain more than one normal seedling from each seed. They also showed that even after root and shoot removal, regeneration of new roots and seedlings occurred. These characteristics could be understood as evolutionary strategies to ensure natural propagation of this species in an unsuitable environment.

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Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination

Cited by 26 publications

References 35 publications

A new approach towards the so-called recalcitrant seeds

A new approach towards the so-called recalcitrant seeds

Exceptional flooding tolerance in the totipotent recalcitrant seeds ofEugenia stipitata

Cutting and regeneration of roots and seedlings from seeds of Eugenia candolleana DC. at different maturity stages

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