Analyses Using SSR and DArT Molecular Markers Reveal that Ethiopian Accessions of White Lupin (&amp;lt;i&amp;gt;Lupinus albus&amp;lt;/i&amp;gt; L.)  Represent a Unique Genepool

Raman, Rosy; Cowley, Raymond; Raman, Harsh; Luckett, David J.

doi:10.4236/ojgen.2014.42012

Cited by 29 publications

(31 citation statements)

References 31 publications

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“…The distribution and patterns of accessions, over different clusters from these three major geographic origins, would suggest future collections of local accessions in those geographic regions with particular emphasis on Awi, followed by East Gojam, and West Gojam for future national collection mission in white lupin. Supportive results that Ethiopian accessions formed a very distinct and separate grouping/gene pool than others, was reported from Australia (Raman et al, 2014).…”

Section: Discussionsupporting

confidence: 52%

Extent and pattern of genetic diversity in Ethiopian white lupin landraces for agronomical and phenological traits

Atnaf¹,

Tesfaye²,

Dagne³

et al. 2015

Afr. Crop Sci. J.

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White lupin (Lupinus albus) is one of four economically important species of the Lupinus genus, and has been traditionally cultivated for thousands of years along the Nile valley, including in Ethiopia. An experiment comprising of 143 Ethiopian White lupin landraces and one genotype from Germany, was undertaken at Merawi in Ethiopia. The objective of the study was to cluster the Ethiopian white lupin accessions into similarity groups and assess the extent and pattern of diversity of the accessions. Data on 10 quantitative agronomic traits were recorded. Landraces significantly differed in most of the traits studied, and a significant number of local accessions performed as high as 5 metric tonnes per hectare of grain yield. Cluster analysis showed that landraces were grouped into seventeen clusters of different sizes, of which five were singletons. Some landraces were grouped together regardless of their geographic origin. On the other hand, landraces from Awi, South Gondar and West Gojam in Ethiopia were distributed over many clusters. Hence, the result did not support a definite relationship between geographic diversity and genetic diversity. Genetic distances between many pairs of clusters were significant, justifying crosses between parents from them to be desirable genetic recombinations and, hence, transgressive segregants.

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Section: Discussionsupporting

confidence: 52%

Extent and pattern of genetic diversity in Ethiopian white lupin landraces for agronomical and phenological traits

Atnaf¹,

Tesfaye²,

Dagne³

et al. 2015

Afr. Crop Sci. J.

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“…Significant intra-specific variations were reported among Ethiopian white lupin landrace accessions (Atnaf et al, 2015b) and also among accessions from other parts of the world (Raman et al, 2014;Rubio et al, 2004) for grain yield, disease resistance and other important agronomic traits. Several researchers studied the relationships among agronomic traits in white lupin (Annicchiarico et al, 2010;Gonzalez-Andres et al, 2007;Rubio et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Genotype by trait biplot analysis to study associations and profiles of Ethiopian white lupin (Lupinus albus L.) landraces

Atnaf¹,

Tesfaye²,

Dagne³

et al. 2017

Aust J Crop Sci

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Limited information is available on trait relations and profiles of white lupin landraces in tropical growing conditions including Ethiopia. The objectives of this study were to understand the relationships among traits, and to document trait profile of Ethiopian white lupin landraces. As a component of this study, two sets of experiments were conducted. The first comprehensive experiment that consisted of 143 accessions collected from major lupin growing areas of Ethiopia was evaluated at Merawi and the second experiment that consisted of 12 selected accessions was evaluated across six locations. In both experiments, significant variations were observed among the accessions for most studied traits. Higher heritability and genetic advance as percent of mean was observed for grain yield, indicating the possibility of improving this trait through selection. Genotype by trait biplots captured 55% -66% of the variations due to genotype by trait interactions. Trait association and trait profile biplots were constructed for highland, mid altitude and low land environments, and across all locations. Different patterns of associations and genotype (accession) by trait interactions were observed in different environments. However, genotype by trait biplots consistently indicated that grain yield had positive associations with most of the traits; especially, with number of pods per plant, plant height and seeds per pod. The study identified some accession with desirable performances as good for specific trait and/or trait groups that could be considered as sources of genes for the traits they have best performed. G8 consistently showed higher grain yield, G2 had higher number of branches and higher number of seeds per plant, G7 had more number of pods per plant and larger seed size and G4 produced longer pods. The accessions used in this study were found to be useful sources for genetic variability for future breeding that targets to improve grain yield and other agronomic traits of white lupin in Ethiopia.

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“…A detailed description of these accessions was given in our previous study [28]. Total genomic DNA was isolated as described in [28] and approximately 100 µg/µL was used for DArT genotyping.…”

Section: Plant Materials and Dna Extractionmentioning

confidence: 99%

“…Details of this array design, platform and genotyping, and scoring of dominant DArT markers have been previously described [29]. Binary data derived from the newer L. albus array was combined with PCR-based markers and DArT data from the multispecies array generated in our previous study [28]. Monomorphic markers were excluded from subsequent analysis.…”

Section: Genotyping and Data Analysismentioning

confidence: 99%

“…The combined dataset of marker scores: PCR-based, meta-genomic Lupinus array [28], and the newer tri-genomic L. albus DArT array, were imported into the SVS suite version 7 software as per the manufacturer's instructions (Golden Helix). Genotype predicted means of PB and PRR resistance scores (phenotypes) were also imported and used in association mapping analysis.…”

Section: Genotype Association Analysismentioning

confidence: 99%

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Localisation of Loci Involved in Resistance to Diaporthe toxica and Pleiochaeta setosa in White Lupin (Lupinus albus L.)

Raman¹,

Vipin²,

Luckett³

et al. 2014

OJGen

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L. albus is an annual grain-legume crop mainly grown for high-protein fodder worldwide but also to produce large seeds for human consumption as a snack-food. In order to make genetic gains in grain yield, assessment of the genetic variation in the germplasm and identification of loci associated with agronomic traits are essential. Phomopsis blight (PB) and Pleiochaeta root rot (PRR), caused by the fungal pathogens Diaporthe toxica and, Pleiochaeta setosa respectively, are two major yield-limiting diseases of the L. albus crop. The extent of genetic diversity in 94 accessions of L. albus comprising: Australian and exotic cultivars, advanced breeding lines, and landraces originating from 26 different countries was determined utilizing PCR-based genic, and microarraybased Diversity Arrays Technology (DArT™), markers. All accessions were evaluated for resistance to PB in two plant tissues (leaves and stems) using either sprayed or injected spore inoculum. A subset of 58 accessions was further evaluated for resistance to PRR by growing seedlings in spore-infested potting mix. The combined data of 724 (50 genic-and 674 DArT) markers were used for cluster analysis. A subset of 324 markers with call rate ≥95% and predicted disease scores of different genotypes were used to identify marker loci accounting for phenotypic variation in PB and PRR resistance using linear regression analysis. Several markers showed significant association with PB or PRR resistance at P < 0.05. Our results showed that favourable alleles for PB and PRR resistance are present in the diverse accessions investigated and they will provide

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Analyses Using SSR and DArT Molecular Markers Reveal that Ethiopian Accessions of White Lupin (<i>Lupinus albus</i> L.) Represent a Unique Genepool

Cited by 29 publications

References 31 publications

Extent and pattern of genetic diversity in Ethiopian white lupin landraces for agronomical and phenological traits

Extent and pattern of genetic diversity in Ethiopian white lupin landraces for agronomical and phenological traits

Genotype by trait biplot analysis to study associations and profiles of Ethiopian white lupin (Lupinus albus L.) landraces

Localisation of Loci Involved in Resistance to <i>Diaporthe toxica</i> and <i>Pleiochaeta setosa</i> in White Lupin (<i>Lupinus albus</i> L.)

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Analyses Using SSR and DArT Molecular Markers Reveal that Ethiopian Accessions of White Lupin (&lt;i&gt;Lupinus albus&lt;/i&gt; L.) Represent a Unique Genepool

Cited by 29 publications

References 31 publications

Extent and pattern of genetic diversity in Ethiopian white lupin landraces for agronomical and phenological traits

Extent and pattern of genetic diversity in Ethiopian white lupin landraces for agronomical and phenological traits

Genotype by trait biplot analysis to study associations and profiles of Ethiopian white lupin (Lupinus albus L.) landraces

Localisation of Loci Involved in Resistance to &lt;i&gt;Diaporthe toxica&lt;/i&gt; and &lt;i&gt;Pleiochaeta setosa&lt;/i&gt; in White Lupin (&lt;i&gt;Lupinus albus&lt;/i&gt; L.)

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Analyses Using SSR and DArT Molecular Markers Reveal that Ethiopian Accessions of White Lupin (<i>Lupinus albus</i> L.) Represent a Unique Genepool

Localisation of Loci Involved in Resistance to <i>Diaporthe toxica</i> and <i>Pleiochaeta setosa</i> in White Lupin (<i>Lupinus albus</i> L.)