Analysis and characterization of the complete genomic sequence of the Chinese strain of hirame rhabdovirus

YingJie, Sun; Zhou, Min; Li, Hong; Yue, Zhiqin; Zeng, Xiaocong; Wang, Zhe

doi:10.1111/j.1365-2761.2010.01218.x

Cited by 18 publications

(15 citation statements)

References 3 publications

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“…In China, the first HIRRV isolation was reported in cultured stone flounder in 2010 [3]. Although there were some cases discovered with similar symptoms in other marine fish, no formal reports on the virus identification have been reported.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China

Zhang

Tang

Sheng

et al. 2017

Virol J

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BackgroundHirame rhabdovirus virus (HIRRV) is a rhabdovirus that causes acute hemorrhage disease in fish culture, resulting in a great economic loss in parts of Asia and Europe.MethodsIn this study, we isolated a virus strain named as CNPo2015 from cultured Japanese flounder in Shandong province, China. Cell isolation, electron microscopic observation, RT-PCR detection and phylogenetic analysis were used for virus identification. Further, artificial infection experiment was conducted for virulence testing.ResultsThe gross signs included abdominal distension, fin reddening and yellow ascitic fluid in the abdominal cavity. Histopathological examination revealed marked cell degeneration and necrosis in the kidney. The tissue homogenates induced obvious cytopathic effects in EPC, FHM and FG cell lines. Electron microscopic observation showed the virus had a bullet-like shape with a capsule membrane. RT-PCR and sequencing analysis revealed that CNPo2015 belonged to the HIRRV with high sequence identity to HIRRV isolates. Infection experiment confirmed that the HIRRV CNPo2015 strain was virulent to flounder juveniles with a LD50 value of 1.0 × 105.9 TCID50/fish.ConclusionIn conclusion, we described the first isolation and characterization of a HIRRV from Japanese flounder in China. This will provide a candidate material for further research on the infection mechanism and preventive strategies of HIRRV.

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Section: Discussionmentioning

confidence: 99%

“…The virus was first described in cultured Japanese flounder in Japan in the early 1980s [1], from where it gradually spread to South Korea and China [2, 3]. However, due to increasing travels and rapid globalization, the outbreak of HIRRV has also been reported in part of Europe [4].…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China

Zhang

Tang

Sheng

et al. 2017

Virol J

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“…; Yingjie et al . ). In Korea, an HIRRV‐like virus was isolated from cultured olive flounder in 1997 (Oh & Choi ) and HIRRV infection was reported in cultured black seabream in 2015 (Kim & Oh ).…”

Section: Introductionmentioning

confidence: 97%

“…; Yingjie et al . ). Thus, partial sequencing of the P gene is useful for studying the molecular epidemiology of HIRRV outbreak in new hosts and locations (Borzym et al .…”

Section: Introductionmentioning

confidence: 97%

Outbreak of hirame rhabdovirus infection in cultured spotted sea bass Lateolabrax maculatus on the western coast of Korea

et al. 2016

Journal of Fish Diseases

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In this study, we determined the cause of a disease outbreak in spotted sea bass, Lateolabrax maculatus reared in culture cages on the western coast of Korea in 2013. The major signs in the diseased fish exhibited were haemorrhaging on the membranes of the abdomen, gastrointestinal organs and opercular gills, as well as an enlarged spleen. No external morphological signs of infection were visible, except for a darkening in colour. No parasites or pathological bacteria were isolated from the diseased fish; however, epithelioma papulosum cyprini (EPC) cells inoculated with tissue homogenates from the diseased fish showed cytopathic effects (CPEs). Virus particles in the EPC cells were bullet-shaped, 185-225 nm long and 70-80 nm wide, characteristic of Rhabdoviridae. Polymerase chain reaction analyses of homogenized tissues from the diseased fish and supernatants of cell cultures with CPEs indicated specific, 553-bp-long fragments corresponding to the matrix protein gene of the hirame rhabdovirus (HIRRV). Phylogenetically, the HIRRV phosphoprotein gene of spotted sea bass was more closely related to phosphoproteins from Chinese and Polish HIRRV strains than from other Korean strains. To our knowledge, this is the first report of HIRRV infection in cultured spotted sea bass.

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“…; Yingjie et al . ). The two full‐length genomes exhibited a very high identity level (99%), the P gene being the most variable (96.9%).…”

Section: Introductionmentioning

confidence: 97%

First isolation of hirame rhabdovirus from freshwater fish in Europe

Borzym

Matras

Maj‐Paluch

et al. 2013

Journal of Fish Diseases

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A rhabdovirus was isolated in cell culture inoculated with tissue material from diseased grayling, Thymallus thymallus (L.), originating from a fish farm affected by a mortality episode in Poland. Diagnostics tests showed that the virus was not related to novirhabdoviruses known in Europe, nor to vesiculovirus-like species, except perch rhabdovirus (PRhV) with which it shared moderate serological relations. However, RT-PCR with PRhV probes gave negative results. To identify the virus, a random-priming sequence-independent single primer amplification was adopted. Surprisingly, two of the obtained sequences exhibited a high identity (>99%) with hirame rhabdovirus (HIRRV), a novirhabdovirus usually found in fish in marine Asiatic countries, for instance Japan, China and Korea. The full-length sequence of the phosphoprotein gene (P) demonstrated a higher identity of the present isolate with HIRRV from China compared with the Korean isolate. An identical viral sequence was also found in brown trout, Salmo trutta trutta L., affected by mortalities in a second farm in the same region, after a likely contamination from the grayling farm. To our knowledge, this is the first report of HIRRV in Europe, and in two hosts from fresh water that have not been described before as susceptible species.

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Analysis and characterization of the complete genomic sequence of the Chinese strain of hirame rhabdovirus

Cited by 18 publications

References 3 publications

Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China

Isolation and identification of a new strain of hirame rhabdovirus (HIRRV) from Japanese flounder Paralichthys olivaceus in China

Outbreak of hirame rhabdovirus infection in cultured spotted sea bass Lateolabrax maculatus on the western coast of Korea

First isolation of hirame rhabdovirus from freshwater fish in Europe

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