Vibrations in the aircraft assembly building will affect the precision of the robotic drilling system. A variable stiffness and damping semiactive vibration control mechanism with quasi-zero stiffness characteristics is developed. The quasi-zero stiffness of the mechanism is realized by the parallel connection of four vertically arranged bearing springs and two symmetrical horizontally arranged negative stiffness elements. Firstly, the quasi-zero stiffness parameters of the mechanism at the static equilibrium position are obtained through analysis. Secondly, the harmonic balance method is used to deal with the differential equations of motion. The effects of every parameter on the displacement transmissibility are analyzed, and the variable parameter control strategies are proposed. Finally, the system responses of the passive and semiactive vibration isolation mechanisms to the segmental variable frequency excitations are compared through virtual prototype experiments. The results show that the frequency range of vibration isolation is widened, and the stability of the vibration control system is effectively improved without resonance through the semiactive vibration control method. It is of innovative significance for ambient vibration control in robotic drilling systems.