Analysis and prediction of ATP concentration in the animal brain under hypoxic conditions

Moshkova, Albina; Khvatova, E. M.; Rusakova, I. A.

doi:10.1134/s1819712409010061

Cited by 3 publications

(2 citation statements)

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“…ATP has also been used as an internal reference because its concentration is relatively stable (~8.2 mM cell water) (76). However, baseline ATP concentration may change in chronic diseases (77)(78)(79), limiting the use of this approach in diseased tissues. Alternatively, many studies have used metabolite concentration ratios such as PCr-to-P i and PCrto-ATP ratios to evaluate metabolic changes in a variety of diseased conditions (4,(80)(81)(82)(83)(84)(85)(86)(87)(88).…”

Section: P-mrsmentioning

confidence: 99%

Assessing tissue metabolism by phosphorous-31 magnetic resonance spectroscopy and imaging: a methodology review

Liu

2017

Quant. Imaging Med. Surg.

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Many human diseases are caused by an imbalance between energy production and demand.Magnetic resonance spectroscopy (MRS) and magnetic resonance imaging (MRI) provide the unique opportunity for in vivo assessment of several fundamental events in tissue metabolism without the use of ionizing radiation. Of particular interest, phosphate metabolites that are involved in ATP generation and utilization can be quantified noninvasively by phosphorous-31 ( 31 P) MRS/MRI. Furthermore, 31 P magnetization transfer (MT) techniques allow in vivo measurement of metabolic fluxes via creatine kinase (CK) and ATP synthase. However, a major impediment for the clinical applications of 31 P-MRS/MRI is the prohibitively long acquisition time and/or the low spatial resolution that are necessary to achieve adequate signal-to-noise ratio. P-MRS/MRI techniques. Applications of these techniques to the investigation of a specific physiology/pathology will be discussed without detailed description. Interested readers are referred to recent review articles on the applications of 31 P-MRS/MRI in metabolic characterization of skeletal muscle (10-12), heart (13), brain (14), and liver (11), as well as in diseases such as cancer (15), heart failure (16), and obesity and diabetes (17,18). Historical perspectiveThe use of 31 P-MRS for metabolic investigations dates back to 1960. Cohn and Hughes were the first to obtain a high-resolution 31 P spectrum of a solution of ATP (19). In addition, they also observed a dependence of the chemical shifts of the phosphorus nuclei of ATP on the pH of the solution. In parallel to the early development of MRI methods by Lauterbur (20), the entire 1970s also witnessed the rapid advance of 31 P-MRS in metabolic investigation of a broad range of biological systems. In 1973, Moon and Richards performed the first 31 P-MRS study that measured intracellular pH in human red blood cells (21). In the following year, Henderson and colleagues obtained the first 31 P spectrum of ATP from human red blood cells (22). At the same time, the Oxford team led by Radda performed the first experiment to acquire 31 P spectra from an intact organ, i.e., the excised and superfused muscle of rat hindlimb (23). The first in vivo 31 P-MRS study was performed on mouse brain by Chance et al. (24). Within the short span of one decade, organelles including mitochondria (25,26) and chromaffin granules (27), cells including Escherichia coli (28), yeast (29), and hepatocytes (30), and organs including skeletal muscle (31,32), heart (33-35), brain (36), kidney (37), and liver (38,39) have all been studied using 31 P-MRS.It is worth noting that most of these organ studies were performed on excised organs to avoid the need for spatial localization. Although Lauterbur has demonstrated the feasibility of imaging water protons (20), it was considered impractical for 31 P imaging of living systems because of the low sensitivity and difficulties with spectral resolution.The 1980s began with the publication of two important studies that aimed a...

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Section: P-mrsmentioning

confidence: 99%

Assessing tissue metabolism by phosphorous-31 magnetic resonance spectroscopy and imaging: a methodology review

Liu

2017

Quant. Imaging Med. Surg.

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“…The method of approximation of empirical dependence was previously tested by us for other parameters of energy metabolism in the brain: for analysis and prognosis of ¿ nal concentration of ATP from activity of mitochondrial enzyme complexes [14]; for evaluation of kinetic characteristics of oxidative phosphorylation in the brain of experimental animals [13]; for prediction of the limits of organism's resistance to hypoxia [8], etc. The use of the method of approximation of empirical dependencies in this study makes it possible to predict mCK activity by calculation methods and to determine ATP content in the brain under extreme conditions.…”

Section: Resultsmentioning

confidence: 99%

Approaches to Prediction of the Adaptive State of the Brain Energetic System under Conditions of Hypoxia

Moshkova¹,

Ерлыкина²,

Sergeeva³

et al. 2010

Bull Exp Biol Med

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Evaluating tissue bioenergetics by phosphorous-31 magnetic resonance spectroscopy

2021

Clinical Bioenergetics

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Analysis and prediction of ATP concentration in the animal brain under hypoxic conditions

Cited by 3 publications

References 4 publications

Assessing tissue metabolism by phosphorous-31 magnetic resonance spectroscopy and imaging: a methodology review

Assessing tissue metabolism by phosphorous-31 magnetic resonance spectroscopy and imaging: a methodology review

Approaches to Prediction of the Adaptive State of the Brain Energetic System under Conditions of Hypoxia

Evaluating tissue bioenergetics by phosphorous-31 magnetic resonance spectroscopy

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